Studies on Functional Regulation in Endogenous Regucalcin, An Intracellular Calcium Signaling Regulatory Protein, in Regucalcin Transgenic Rats

细胞内钙信号调节蛋白 Regcalcin 转基因大鼠内源性 Regcalcin 功能调节的研究

• 批准号: 13672292
• 负责人: YAMAGUCHI Masayoshi
• 金额: $ 2.3万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672292/
• 关键词: regucalcin; transgenic rats; osteoporosis; hyperlipidemia; 高アルブミン血症; 高脂血症; 血清脂質; 血清アルブミン; プロテインホスファターゼ; 遺伝子発現; 生体調節; カルシウム; 肝臓; 骨; 大脳

Regucalcin, which was discovered by Yamaguchi as a calcium-binding protein in 1978 years, has endogenous regucalcin was investigated by using regucalcin transgenic (RC-Tg) rats. The body weight of RC-Tg rats was significantly suppressed with increasing age. Endogenous regucalcin was markedly expressed in the organ tissues of the Tg rats. Especially, its expression was remarkable in the female rats rather than male rats, because the regucalcin gene is localized on chromosome X. The role of regucalcin in the regulation of cellular functions, which were found in normal rats, was also demonstrated in the organ tissues of RC-Tg rats. Bone loss was found to induce in the femoral tissues of RC-Tg rats with increasing age. This was based on stimulation of osteoclastic bone resorption. Regucalchin had a stimulatory effect on osteoclastic cell formation from bone marrow cells. Futhermore, hyperlipidemia was found to induce in RC-Tg rats with increasing. Also, a significant increase in serum albumin was induced in RC-Tg rats. RC-Tg rats were usfull as rat model for osteoporosis and hyperlipidemia with increasing age.

利用转Regucalin基因(RC-TG)大鼠研究了内源性Regucalin，它是山口在1978年发现的一种钙结合蛋白。RC-TG大鼠体重随增龄而明显抑制。内源性调节素在TG大鼠的脏器组织中有明显表达。特别是由于Regucalcin基因定位在X染色体上，所以在雌性大鼠中的表达显著高于雄性大鼠。在RC-TG大鼠的器官组织中也证实了Regucalcin在细胞功能调节中的作用，这在正常大鼠中也有发现。随着年龄的增长，RC-TG大鼠的股骨组织出现骨丢失。这是基于对破骨细胞性骨吸收的刺激。Regucalin对骨髓细胞形成破骨细胞有促进作用。此外，高脂血症对RC-TG大鼠有诱导作用，且呈递增趋势。同时，RC-TG大鼠血清白蛋白也显著升高。RC-TG大鼠作为增龄骨质疏松和高脂血症大鼠模型。

项目成果

Ma ZJ, Yamaguchi M: "Regulatory effect of regucalcin on nitric oxide synthase activity in rat kidney cortex cytosol : Role of..."International Journal of Molecular Medicine. 12. 201-206 (2003)

Ma ZJ，Yamaguchi M：“调节钙素对大鼠肾皮质细胞质中一氧化氮合酶活性的调节作用：......的作用”国际分子医学杂志。

Yamaguchi M, Takahashi H, Tsurusaki Y: "Suppressive role of endogenous regucalcin in the enhancement of nitric oxide synthase activity in liver cytosol of normal and regucalcin transgenic rats."Journal of Cellular Biochemistry. 88. 1226-1234 (2003)

Yamaguchi M、Takahashi H、Tsurusaki Y：“内源性调节钙素在正常和调节钙素转基因大鼠肝细胞质中一氧化氮合酶活性增强中的抑制作用。”细胞生物化学杂志。

Tsurusaki Y, Yamaguchi M: "Role of endogenous regucalcin in transgenic rats : Suppression of protein tyrosine phosphatase and ribonucleic acid synthesis activities in liver nucleus."International Journal of Molecular Medicine. 12. 207-211 (2003)

Tsurusaki Y，Yamaguchi M：“内源性调节钙蛋白在转基因大鼠中的作用：抑制肝核中的蛋白酪氨酸磷酸酶和核糖核酸合成活性。”国际分子医学杂志。

Ichikawa E, Tsurusaki Y, Yamaguchi M: "Suppressive effect of regucalcin on protein phosphatase activity in the heart cytosol of normal and regucalcin transgenic rats."International Journal of Molecular Medicine. 13. 289-293 (2004)

Ichikawa E、Tsurusaki Y、Yamaguchi M：“调节钙素对正常和调节钙素转基因大鼠心脏细胞质中蛋白磷酸酶活性的抑制作用。”国际分子医学杂志。

Tsurusaki Y., Yamaguchi, M: "Role of endogenous regucalcin in transgenic rats : Suppression of protein tyrosine phosphatase and..."International Journal of Molecular Medicine. 12. 207-211 (2003)

Tsurusaki Y.，Yamaguchi，M：“内源性调节钙蛋白在转基因大鼠中的作用：蛋白酪氨酸磷酸酶的抑制和......”国际分子医学杂志。