Regulatory role of ca^<2+> -binging protein requcalcin in signal transduction system and its mechanism

ca^2结合蛋白requcalcin对信号转导系统的调节作用及其机制

• 批准号: 04671362
• 负责人: YAMAGUCHI Masayoshi
• 金额: $ 1.41万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671362/
• 关键词: Regucalcin; Ca^<2+>-binding protein; Signal transduction system; liver plasma menbrane (Ca^<2+>-Mg^<2+>)-ATPase; Nuclear Ca^<2+> transport system; Nuclear Ca2+-ATPase; 核機能; Ca^<2+>-ATPase; アラチドン酸; NAD^+; 肝細胞機能; (Ca^<2+>-Mg^<2+>)-ATPアーゼ; カルシウムト

The requlatory role of Ca^<2+> -binding protein requcalcin in signal trasnduction system of liver cells was investigated, and the following results were obtained.1) Requcalcin activates directly (Ca^<2+>-Mg^<2+>)-ATPase in liver plasma membranes, an the activation is not involved in the Ca^<2+>-dependent phosphorylation of the enzyme.2) The activating mechanism of requcalcin on (Ca^<2+>-Mg^<2+>)-ATPase is not involved on GTP-binding protein which modulates the receptor-mediated hormonal effect in rat liver plasma menbranes.3) Regucalcin stimulates Ca^<2+> release from liver nuclei, and the effect is not influenced by calmodulin antagonist, suggesting that regucalcin can regulate the Ca^2 transport system in rat liver nuclei.4) Arachidonic acid inhibited Ca^<2+> uptake and stimulated Ca^<2+> release in rat liver nuclei.5) NAD^+ has an inhibitory effect on nuclear Ca^<2+> uptake.6) Liver nuclei were Ca^<2+> -ATPase which can taken up Ca^<2+>.Rgucalcin had no effect on the enzyme activity, although arachidonic acid and NAD^+ inhibited the enzyme activity.

研究Ca^<2+> -结合蛋白requcalcin在肝细胞信号转导系统中的调节作用，得到以下结果：1) Requcalcin直接激活(Ca^<2+>-Mg^<2+>)- atp酶，该激活不参与Ca^<2+>依赖性酶磷酸化。2)常钙素对(Ca^<2+>-Mg^<2+>)- atp酶的激活机制不参与调节受体介导的大鼠肝质膜激素效应的gtp结合蛋白。3) Regucalcin刺激Ca^<2+>从肝核释放，且不受钙调素拮抗剂的影响，提示Regucalcin可调节大鼠肝核Ca^2转运系统。4)花生四烯酸抑制大鼠肝核Ca^<2+>摄取，刺激Ca^<2+>释放。5) NAD^+对细胞核Ca^<2+>摄取有抑制作用。6)肝核为Ca^<2+> - atp酶，可吸收Ca^<2+>。花生四烯酸和NAD^+对酶活性有抑制作用，而芦黄素对酶活性无影响。

项目成果

Kimiko,Oishi: "Regulatory effect of arachidonic acid on the calcium transport system in rat liver nuclei" Biochemical Pharmacology. 45. 1471-1475 (1993)

Kimiko，Oishi：“花生四烯酸对大鼠肝核钙转运系统的调节作用”生化药理学。

Kimiko Oishi: "Regulatory effect of arachidonic acid on the calcium transport system in rat liver nuclei." Biochemical Pharmacology. 45. 1471-1475 (1993)

Kimiko Oishi：“花生四烯酸对大鼠肝细胞核钙转运系统的调节作用。”

Hiroko Takahashi: "Regucalcin modulates hormonal effect on (Ca^<2+>-Mg^<2+>)-ATPase activity in rat liver plasma menbranes." Molecular and Cellular Biochemistry. 125. 171-177 (1992)

Hiroko Takahashi：“Reguccalcin 调节大鼠肝血浆膜中 (Ca^2>-Mg^<2>)-ATPase 活性的激素效应。”

Hiroko Takahashi: "Effect of calcium-binding protein reducalcin on Ca^<2+> transport system in rat livver nuclei : stimulation of Ca^<2+> release." Molecular and Cellular Biochemistry. 113. 63-70 (1992)

Hiroko Takahashi：“钙结合蛋白 reducalcin 对大鼠肝核 Ca^2 转运系统的影响：刺激 Ca^2 释放。”

Hiroko,Takahashi Masayoshi,Yamaguchi: "Regulatory effect of regucalcin on (Ca^<2+>-Mg^<2+>)-ATPase in rat liver plasma membranes:Conparison with the activation by Mn^<2+> and Co^<2+>" Moleculan and Cellulan Biochemistry. (1993)

Hiroko、Takahashi Masayoshi、Yamaguchi：“雷古钙素对大鼠肝质膜 (Ca^<2>-Mg^<2>)-ATPase 的调节作用：与 Mn^<2> 和 Co^<2> 激活的比较