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Expression of a novel Ca^<2+>-binding protein regucalcin and rlation to its physiologic role

新型Ca^2结合蛋白regcalcin的表达及其生理作用的关系

基本信息

批准号：
06672193
负责人：
YAMAGUCHI Masayoshi
金额：
$ 1.54万
依托单位：
University of Shizuoka
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672193/
关键词：
Regucalcin Calcium-binding protein Gene Calcium Rat liver カルシウム結合蛋白質遺伝子発現

项目摘要

The expression of a novel calcium-binding protein regucalcin and relation to its physiologic role was investigated. The follwing results were obtained.1. Rat regucalcin gene consisted in 7 exons. The gene was determined by direct R-banding fluorescence in situ hybridization method with the 19.9 kb clone containing four exons. The regucalcin gene was localized on rat chromosome Xq11.1-12 proximal end.2. Regucalcin gene was widely conserved among higher animals including human, monkey, rat, mouse, dog, bovine, rabbit and chicken. The gene was not found in yeast. Regucalcin mRNA was predominantly expressed in rat and mouse, although the expression was also seen in human, bovine and chicken.3. The expression of regucalcin mRNA in the liver was elevated by refeeding of fasted rats. This expression was involved in the action of insulin and/or calcium as stimulating factors.4. The expression of hepatic regucalcin mRNA was decreased by phenobarbital administration in rats.5. The concentraition of regucalcin in the liver was clearly increased by calcium administration in rats.6.The expression of hepatic regucalcin mRNA was enhanced in regenerating rat liver, suggesting that regucalcin plays a role in proliferation of liver cells.7.1n the transplantable Morris hepatoma cells, the regucalcin mRNA was markedly expressed, while the albumin mRNA was expressed only slightly.

研究了一种新型钙结合蛋白调节钙蛋白的表达及其与其生理作用的关系。得到以下结果： 1．大鼠regcalcin基因由7个外显子组成。该基因通过直接R显带荧光原位杂交方法与含有四个外显子的19.9 kb克隆进行测定。 regucalcin基因定位于大鼠染色体Xq11.1-12近端。2． Regcalcin基因在人、猴、大鼠、小鼠、狗、牛、兔和鸡等高等动物中广泛保守。在酵母中未发现该基因。 Regcalcin mRNA主要在大鼠和小鼠中表达，但在人、牛和鸡中也有表达。3．禁食大鼠重新进食后，肝脏中调节钙素 mRNA 的表达升高。该表达参与了胰岛素和/或钙作为刺激因子的作用。 4．苯巴比妥可降低大鼠肝脏regcalcin mRNA的表达。 5．给予大鼠钙剂后，肝脏中Reguccalcin浓度明显升高。6.再生大鼠肝脏中Reguccalcin mRNA表达增强，提示regcalcin在肝细胞增殖中发挥作用。7.1在可移植的Morris肝癌细胞中，regcalcin mRNA明显表达，而白蛋白mRNA仅轻微表达。