Studies on Liver Cellular Functions by a Novel Ca^<2+>-Binding Protein Regucalcin

新型Ca^<2>-结合蛋白Reguccalcin对肝细胞功能的研究

• 批准号: 02671006
• 负责人: YAMAGUCHI Masayoshi
• 金额: $ 0.45万
• 依托单位: University of Shizuoka, Graduate School of Nutritional Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02671006/
• 关键词: regucalcin; Ca^<2+>-binding protein; Liver cell function regulation; calcium ion

1. Tissue distribution of regucalcinRabbit-anti-regucalcin antiserum, which was raised against regucalcin conjugated by glutaraldehyde to bovine serum albumin, was applied to glutaraldehyde-fixed whole mounts and subsequently visualized using the peroxidase-antiperoxidase methods. Rat hepatic regucalcin immunoreactivity was most pronounced in the liver of rats, while it. was not seen in the duodenum, testicle, spleen, lung and smooth muscle. This was confirmed by the methods of enzyme-immunoassay and Northern bloting for MRNA of regucalcin gene.2. Regulation of liver cell function by regucalcinIn rat liver cytosol, regucalcin completely prevented the activation of Ca^<2+>/ calmodulin-dependent cyclic AMP phosphodiesterase by Ca^<2+> and the inhibition of deoxyuridine 5'-triphosphatase by Ca^<2+>. Also, DNA fragmentation in isolated rat liver nuclei was activated by Ca^<2+>. This activation was completely blocked by the presence of regucalcin. moreover, the liver nuclei is Ca^<2+> transport systems. Especially, regucalcin stimulated Ca^<2+> release from rat liver nuclei, but not it had no effect on Ca^<2+> uptake.

1. 雷古钙素的组织分布兔抗雷古钙素抗血清是针对戊二醛与牛血清白蛋白缀合的雷古钙素而产生的，应用于戊二醛固定的整体上，随后使用过氧化物酶-抗过氧化物酶方法进行可视化。大鼠肝脏调节钙素免疫反应性在大鼠肝脏中最为明显，而其。十二指肠、睾丸、脾、肺和平滑肌中未见。 regucalcin基因mRNA的酶联免疫分析和Northern blotting方法证实了这一点。 2．雷古钙素对肝细胞功能的调节在大鼠肝细胞质中，雷古钙素完全阻止Ca ^ 2+ 对Ca ^ 2+ /钙调蛋白依赖性环AMP磷酸二酯酶的激活以及Ca ^ 2+ 对脱氧尿苷5'-三磷酸酶的抑制。此外，分离的大鼠肝核中的DNA片段化被Ca 2+ 激活。这种激活被调节钙素的存在完全阻断。而且，肝细胞核是Ca^2+转运系统。特别是，雷古钙素刺激大鼠肝核中Ca^2+的释放，但它对Ca^2+的摄取没有影响。

项目成果

Masayoshi Yamaguchi: "Inhibitory Effect of CalciumーBinding Protein Regucalcin on Ca^<2+>/calmodulinーDependent Cyclic Nucleotide Phosphodiesterase Activity in Rat Liver Cytosol." Mollecular and Cellular Biochemistry. 100. (1991)

Masayoshi Yamaguchi：“钙结合蛋白 Regcalcin 对大鼠肝细胞溶胶中 Ca^<2+>/钙调素依赖性环核苷酸磷酸二酯酶活性的抑制作用。”(1991)。

M.Yamaguchi: "Reversible ettect of calciumーbinding protein regucalcin on the Ca^<2+>ーinduced inhibition of deoxyuridine 5^1ーtriphosphatase actirity in rat liver cytosal" Molecular and Cellular Biochemistry. (1992)

M. Yamaguchi：“钙结合蛋白调节钙蛋白对Ca^2+诱导的大鼠肝细胞内脱氧尿苷5^1-三磷酸酶活性抑制的可逆效应”分子与细胞生物化学(1992)。

Masayoshi Yamaguchi: "Immunohistochemical Demonstration of CalciumーBinding Protein Regucalcin in the Tissues of Rats:The Protein Localizes in Liver and Brain" Chemical and Pharmaceutical Bulletin. 39,No.6. (1991)

Masayoshi Yamaguchi：“大鼠组织中钙结合蛋白 Regcalcin 的免疫组织化学论证：蛋白质定位于肝脏和大脑”《化学与药物通报》39，第 6 期。（1991 年）

M.Yamaguchi: "Jene expression of regucalcin;The mRNA is increared by calcium adnimistration." FEBS Letters. (1992)

M.Yamaguchi：“regucalcin 的表达；通过给予钙来增加 mRNA。”

Masayoshi Yamaguchi: "Inhibitory Effect of CalciumーBinding Protein Regucalcin on Ca^<2+>ーActivated DNA Fragwentation in Rat Liver Nuclei." FEBS Letters. (1991)

Masayoshi Yamaguchi：“钙结合蛋白 Regcalcin 对大鼠肝细胞核中 Ca^2+ 激活的 DNA 断裂的抑制作用”(1991)。