Phosphorylation in the cyanobacterial circadian clock

蓝藻生物钟的磷酸化

• 批准号: 13680778
• 负责人: IWASAKI Hideo
• 金额: $ 2.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680778/
• 关键词: circadian; biological clock; phosphorylation; Kai; Sas A; kai; 概日リズム; シアノバクテリア

Circadian rhythms have been observed in many organisms at a variety of physiological processes. Cyanobacteria are the simplest organisms to exhibit circadian rhythms, and provide a well-established molecular genetic and physiological model system. In the current study, we revealed that a master clock element protein, KaiC, exhibits autokinase/autophosphatase activities, which are dramatically modified by associating clock proteins, KaiA and KaiB. Relationship among circadian dynamics of protein-protein interactions among clock proteins, phosphorylation of KaiC and their cellular localization will be a basis for further functional analyses on the mechanism to generate circadian rhythms in the prokaryote.

在许多生物体的各种生理过程中都观察到昼夜节律。蓝藻是表现昼夜节律的最简单的生物，并提供了一个完善的分子遗传和生理模型系统。在目前的研究中，我们发现主时钟元件蛋白KaiC具有自激酶/自磷酸酶活性，其活性被相关时钟蛋白KaiA和KaiB显著地修饰。时钟蛋白之间相互作用的昼夜动力学、KaiC的磷酸化及其细胞定位之间的关系将为进一步分析原核生物产生昼夜节律的机制提供功能基础。

项目成果

H.Iwasaki, T.Nishiwaki, Y.Kitayama, M.Nakajima, T.Kondo: "KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria"Proc.Natl.Acad.Sci.. 99. 15788-15793 (2002)

H.Iwasaki、T.Nishiwaki、Y.Kitayama、M.Nakajima、T.Kondo：“蓝藻中昼夜节律循环中 KaiA 刺激的 KaiC 磷酸化”Proc.Natl.Acad.Sci.. 99. 15788-15793 (2002)

北山陽子: "KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial clock system"EMBO Journal. 22. 2127-2134 (2003)

Yoko Kitayama：“KaiB 在蓝细菌时钟系统中充当 KaiC 磷酸化的衰减器”EMBO 杂志 22. 2127-2134 (2003)。

H.Iwasaki, T.Kondo: "Histidine kinases in the cyanobacterial circadian system, Histidine kinases, Book Chapter"Academic Press. 297-311 (2003)

H.Iwasaki，T.Kondo：“蓝藻昼夜节律系统中的组氨酸激酶，组氨酸激酶，书籍章节”学术出版社。

H.Kageyama, T.Kondo, H.Iwasaki: "Circadian formation of clock protein complexes by KaiA, KaiB, KaiC and SasA in cyanobacteria"J.Biol.Chem.. 278. 2388-2395 (2003)

H.Kageyama、T.Kondo、H.Iwasaki：“蓝藻中 KaiA、KaiB、KaiC 和 SasA 形成时钟蛋白复合物”J.Biol.Chem.. 278. 2388-2395 (2003)

Y.Kitayama, H.Iwasaki, T.Nishiwaki, T.Kondo: "KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial clock system"EMBO J.. 22. 2127-2134 (2003)

Y.Kitayama、H.Iwasaki、T.Nishiwaki、T.Kondo：“KaiB 在蓝藻生物钟系统中充当 KaiC 磷酸化的衰减器”EMBO J.. 22. 2127-2134 (2003)