The qualitative and quantitative analysis of olfactory glomerulus and nidus in the olfactory bulb

嗅球内嗅球和巢的定性和定量分析

• 批准号: 13680823
• 负责人: KOSAKA Katsuko
• 金额: $ 2.37万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680823/
• 关键词: Anatomy; Cell Tissue; Neuroscience; Brain Neuron; Confocal laser microscopy

We investigated immunohistochemically the main olfactory bulbs (MOBs) of 7 mammalian species, focusing on two points: 1) whether nidi, particular spheroidal synaptic regions containing many GABAergic calbindin D28K-immunoreactive (CB-IR) elements we previously reported in the laboratory musk shrew MOBs, are also present in other animals, and 2) whether the compartmental organization of glomeruli and two types of periglomerular cells we proposed in the rat MOBs, are general in the MOBs, of these animals. We used two insectivores, moles and hedgehogs, tree shrews, bats and mice as well as laboratory musk shrews and rats. We found discrete nidi and the nidal layer only in the mole and laboratory shrew MOBs, where observed none or a few olfactory nerves (Ons) extruding from glomeruli. These results suggest that the nidi and related distinctive organization might be specific to only some species of insectivores.On the other hand, we recognized the apparent compartmental organization of glomeruli and two types of periglomerular cells in the MOBs of all of the mammals we examined, indicating these structural features are common to the MOBs of various mammals and thus might be important organizational principles of the MOBs.

我们对7种哺乳动物的主要嗅球（MOBs）进行了化学研究，重点是两点：（1）在实验室发现的麝香鼩 MOBs中存在的含有GABA能calbindin D28 K免疫反应（CB-IR）元件的突触巢（nidi）是否也存在于其他动物中;（2）大鼠MOB中肾小球的室性结构和我们提出的两种肾小球周围细胞是否在这些动物的MOB中普遍存在。我们使用了两种食虫动物，鼹鼠和刺猬，树鼩，蝙蝠和小鼠以及实验室麝香鼩和大鼠。我们只在鼹鼠和实验室鼩的MOB中发现了离散的巢和巢层，在那里观察到没有或少数嗅神经（Ons）从肾小球中伸出。这些结果表明，巢和相关的独特的组织可能是特定的一些物种的食虫动物。另一方面，我们认识到，在我们检查的所有哺乳动物的MOBs的肾小球和两种类型的肾小球周围细胞的明显的房室组织，表明这些结构特征是共同的MOBs的各种哺乳动物，因此可能是重要的组织原则的MOBs。

项目成果

Kosaka K., Akita Y., Toida K., Kosaka T.: "Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type 2 periglomerular neurons"J. Comp. Neurol.. 440. 219-235 (2001)

Kosaka K.、Akita Y.、Toida K.、Kosaka T.：“二尖瓣细胞肾小球内树突簇的结构及其与嗅觉神经末梢和钙结合蛋白免疫反应性 2 型肾小球周围神经元的接触”J。

K.Kosaka, T.Kosaka: "Nidus and Tasseled cell : Distinctive neuronal organization of the main olfactory bulb of the laboratory musk shrew (Suncus murinus)."Journal of Comparative Neurology. 430. 542-561 (2001)

K.Kosaka、T.Kosaka：“巢和缨细胞：实验室麝鼩（Suncus murinus）主要嗅球的独特神经元组织。”比较神经学杂志。

小坂克子: "'嗅球糸球体の定性的、定量的形態解析',ブレインサイエンス・レビュー2001"医学書院/学術雑誌. 292 (2001)

Katsuko Kosaka：“‘嗅球肾小球的定性和定量形态分析’，脑科学评论 2001”Igaku Shoin/Academic Journal 292 (2001)。

Kosaka K., Aika Y., Toida K., Kosaka T: "Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type 2 periglomerular neurons"J.Comp.Neurol. 440. 219-235 (2001)

Kosaka K.、Aika Y.、Toida K.、Kosaka T：“二尖瓣细胞肾小球内树突簇的结构及其与嗅觉神经末梢和钙结合蛋白免疫反应性 2 型肾小球周围神经元的接触”J.Comp.Neurol。

K.Kosaka, K.Toida, Y.Aika, T.Kosaka: "Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type periglomeruIar neurons"Journal of Comparative Neurology. 440. 218-235 (2

K.Kosaka、K.Toida、Y.Aika、T.Kosaka：“二尖瓣细胞肾小球内树突簇的结构及其与嗅觉神经末梢和钙结合蛋白免疫反应型肾小球周围神经元的接触”比较神经病学杂志。