Morphological Analyses of the Glomerulus in the Main Olfactory Bulb.

主嗅球肾小球的形态分析。

• 批准号: 15300115
• 负责人: KOSAKA Katsuko
• 金额: $ 9.47万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300115/
• 关键词: Olfactory bulb; interneuron; GABA; gap junction; synapse; confocal laser scanning microscope; electron microscope; 介在ニューロン; 主ニューロン; 免疫細胞化学; レーザー顕微鏡; 嗅覚伝達

Glomeruli of the main olfactory bulb (MOB) are now considered to serve as functional units in the olfactory information processing. Our previous analyses of the rat MOB revealed the compartmental organization of glomeruli and two types of periglomerular (PG) cells, type 1 (PG1)and type 2 (PG2) PG cells. We found that olfactory nerve axon terminals made synaptic contacts with dendritic tufts of both projection neurons (mitral and tufted cells) and PG1, but not of PG2, cells in the rat MOB. In the present project we extended our investigation to the fine structural organization of the glomeruli of mice (including genetically manipulated mice) and other mammals. Using the confocal laser scanning microscope and electron microscope, we found that the colocalization relationship of chemical substances in mouse PG cells was different from that of the rat PG cells reported previously.Furthermore, we confirmed the compartmental organization of the glomeruli and the presence of two types of PG cells in the MOB of bats, tree shrews, hedgehogs, moles, musk shrews and tenrecs. These structural features are therefore considered to be common and important organizational principles of the MOBs. We also exhibited complex neuronal interactions via chemical synapses and gap junctions in the glomerulus.

主要嗅球的肾小球被认为是嗅觉信息处理的功能单位。我们以前对大鼠肾小球的分析揭示了肾小球和两种类型的肾小球周围(PG)细胞的隔室组织，即1型(PG1)和2型(PG2)PG细胞。我们发现，嗅神经轴突终末与投射神经元(二尖瓣细胞和簇状细胞)和PG1细胞的树突束有突触联系，但与PG2细胞的树突束没有联系。在本项目中，我们将我们的研究扩展到小鼠(包括基因操纵的小鼠)和其他哺乳动物的肾小球的精细结构组织。利用激光共聚焦扫描显微镜和电子显微镜，我们发现化学物质在小鼠PG细胞中的共存关系与以前报道的大鼠PG细胞中的不同。此外，我们还证实了蝙蝠、树鼠、刺猬、鼹鼠、麝鼠和田鼠肾小球的区间化组织以及两种PG细胞的存在。因此，这些结构特征被认为是暴徒的共同和重要的组织原则。我们还通过肾小球中的化学突触和缝隙连接展示了复杂的神经元相互作用。

项目成果

Organization of the main olfactory bulbs of some mammals: Musk shrews, moles, hedgehogs, tree shrews, bats, mice, and rats

• DOI: 10.1002/cne.20004
• 发表时间: 2004-04
• 期刊: Journal of Comparative Neurology
• 影响因子: 2.5
• 作者: K. Kosaka;T. Kosaka

匂いと香りの科学(澁谷達明、市川真澄 編著)

气味与香味的科学（涉谷龙明、市川真澄编）

• 发表时间: 2007
• 作者: 関 洋一;櫻井健志;神崎亮平
• 通讯作者: 神崎亮平

T.Kosaka, K.Kosaka: "The organization of the main olfactory bulbs of some mammals ; musk shrews, moles, hedgehogs, tree shrews, bats, mice and rats"Journal of Comparative Neurology. (in press).

T.Kosaka、K.Kosaka：“某些哺乳动物的主要嗅球的组织；麝香鼩、鼹鼠、刺猬、树鼩、蝙蝠、小鼠和大鼠”《比较神经学杂志》。

Structural organization of the glomerulus in the main olfactory bulb

• DOI: 10.1093/chemse/bjh137
• 发表时间: 2005-01-01
• 期刊: CHEMICAL SENSES
• 影响因子: 3.5
• 作者: Kosaka, T;Kosaka, K
• 通讯作者: Kosaka, K

Investigation on the Staining Condition of Post-embedding Immunogold Labeling at the Electron Microscopic Level ; GABA Immunocytochemistry in the Mouse Olfactory Bulb.

电镜水平下包埋后免疫金标记染色情况的研究；

• 发表时间: 2005
• 期刊: Memoirs Dep.of Health Scis.Sch.of Med Kyushu Univ. 6
• 作者: Noda;S.;Kosaka;K
• 通讯作者: K