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Role of calcium-permeable AMPA receptors in glial cells

钙渗透性 AMPA 受体在神经胶质细胞中的作用

基本信息

批准号：
13680862
负责人：
TSUZUKI Keisuke
金额：
$ 2.05万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

We found calcium-permeable AMPA receptors for the first time in rat hippocampal culture (lino et al., J. Physiol., 424 : 151-165, 1990). Now it is known that receptor is widely distributed not only in neurons but also in glial cells. In particular, Bergmann glia in the cerebellum abundantly expresses these receptors. In order to change the calcium permeable AMPA receptors into impermeable ones, transgenic mice that expressed GluR2 in Bergmann glia were constructed in this study. First, we made a plasmid vector that express GluR2_<flip> of the rat under the control of the human glial fibrillary acid protein (GFAP) promoter. Then the expression unit was injected into fertilized eggs of the mouse. GluR2 expression unit was transferred into seven offspring. These transgenic mice were backcrossed with C57BL6 mice for eight generations. The transgenic mice have smaller brains compared with wild type mice. Matching with the region of GFAP, GluR2 were strongly expressed in cerebellar cortex, forming patchy wedges, which were never observed in wild type mice. Examination of cerebellar function is under work.

我们首次在大鼠海马培养物中发现了钙渗透性AMPA受体（lino等人，生理学杂志，424：151-165，1990）。目前已知受体不仅广泛分布于神经元，而且广泛分布于神经胶质细胞。特别是，小脑中的Bergmann神经胶质细胞大量表达这些受体。为了将钙渗透性AMPA受体转化为非钙渗透性AMPA受体，本研究构建了在Bergmann胶质细胞中表达GluR 2的转基因小鼠。首先，我们构建了<flip>人胶质细胞酸性蛋白（GFAP）启动子调控下表达大鼠GluR 2_的质粒载体。然后将表达单位注射到小鼠的受精卵中。将GluR 2表达单位转移到7个后代中。这些转基因小鼠与C57 BL 6小鼠回交8代。与野生型小鼠相比，转基因小鼠的大脑更小。GluR 2与GFAP区域相匹配，在小脑皮质呈片状楔形强表达，这在野生型小鼠中从未观察到。小脑功能检查正在进行中。

项目成果

期刊论文数量（17）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Yutaka Saito, Keisuke Tsuzuki, Nobuaki Yamada, Haruo Okado, Akiko Miwa, Fumio Goto, Seiji Ozawa: "Transfer of NMDAR2 cDNAs increases endogenous NMDAR1 protein and induces expression of functional NMDA receptors in PC12 cells"Brain Res. Mol. Brain Res.. 11

Yutaka Saito、Keisuke Tsuzuki、Nobuaki Yamada、Haruo Okado、Akiko Miwa、Fumio Goto、Seiji Ozawa：“NMDAR2 cDNA 的转移会增加内源性 NMDAR1 蛋白并诱导 PC12 细胞中功能性 NMDA 受体的表达”Brain Res。