Evaluation of dynamic changes of nitric oxide and oxidative stress in hemodialysis patients

血液透析患者一氧化氮及氧化应激动态变化评价

• 批准号: 13680961
• 负责人: MOCHIZUKI Seiichi
• 金额: $ 2.05万
• 依托单位: Kawasaki College of Allied Health Professions
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680961/
• 关键词: hemodialysis; nitric oxide (NO); oxidative stress; superoxide; L-arginine; asymmetric dimethylarginine (ADMA); tetrahydrobiopterin (BH_4); oxidized LDL; スーパーオキサイド; テトラハイドロビオプテリン(BH_4); asymmetric dimethylarginine(ADMA); 血液汚染; 高血圧; ADMA; センサー

Plasma concentrations of oxidized LDL and ADMA, both of which are markers of oxidative stress were measured. OxyLDL was significantly higher than healthy group and increased further by hemodialysis (HD). ADMA level was higher than healthy group before HD and gradually decreased in the time course of HD.Next, we evaluated the plasma levels of NO-related factors in pathophysiological model rats (hypertension and diabetes mellitus). In both groups, plasma BH_4 level decreased, plasma ADMA level increased, and myocardial DDAH (ADMA hydrolase) activity decreased. Superoxide production of control rats did not differ among heart, kidney and liver, while it decreased significantly in those organs of hypertensive rats.Systemic NO production rate in HD patients was estimated and the rate during HD was significantly higher than in the inter-HD period. There was a good linear correlation between L-arginine/ADMA and systemic NO production rate. Also the same between BH_4/BH_2 and systemic NO production rate. From a clinical viewpoint, variations of these parameters would affect significantly the NO production during HD, and thus control of these factors and other methods should be investigated further.

测量氧化LDL和ADMA的血浆浓度，这两者都是氧化应激的标志物。OxyLDL明显高于健康组，且经血液透析（HD）进一步升高。HD前ADMA水平高于健康组，随着HD时间的延长，ADMA水平逐渐下降。两组血浆BH_4水平均降低，血浆ADMA水平均升高，心肌DDAH（ADMA水解酶）活性均降低。对照组大鼠心、肾、肝组织中的超氧化物生成量无明显差异，而高血压组大鼠心、肾、肝组织中的超氧化物生成量明显减少。L-精氨酸/ADMA比值与NO生成率呈良好的线性相关。BH_4/BH_2与系统NO生成速率的关系也基本相同。从临床的角度来看，这些参数的变化将显着影响血液透析过程中NO的产生，因此，这些因素的控制和其他方法应进一步研究。

项目成果

Mochizuki S, et al.: "Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans"Heart.Vessels. 15. 274-279 (2000)

Mochizuki S 等人：“饮食控制对血浆硝酸盐水平的影响以及人体基础全身一氧化氮生成率的估计”Heart.Vesses。

Miyasaka T, et al.: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in dialysate"ASAIO J.. 48. 389-393 (2002)

Miyasaka T 等人：“开发用于透析液中内毒素测量的无污染 6 阀注射器在线监测系统”ASAIO J.. 48. 389-393 (2002)

Kosuke Endo: "Development of a superoxide sensor by immobilization of superoxide dismutase"Sensors and Actuator. 83. 30-34 (2002)

Kosuke Endo：“通过固定超氧化物歧化酶开发超氧化物传感器”传感器和执行器。

Takehiro Miyasaka: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in"ASAIO Journal. 48. 389-393 (2002)

Takehiro Miyasaka：“开发用于内毒素测量的无污染 6 阀注射器在线监测系统”，发表于《ASAIO 杂志》。

望月精一: "冠循環のバイオメカニクス(梶谷文彦編著):ノート(メカノストレスとNO、EDHF、CO、PGI_2)"コロナ社. 222 (2001)

Seiichi Mochizuki：“冠状动脉循环的生物力学（由 Fumihiko Kajitani 编辑）：注释（机械应力和 NO、EDHF、CO、PGI_2）” Coronasha 222 (2001)。