Development of Micro-Probe for Continuous Sampling of Interstitial Fluid in Myocardium for Evaluation of Physiological Circulation, Control, and Mass Transport.

开发用于对心肌间质液进行连续采样的微型探针，以评估生理循环、控制和质量运输。

• 批准号: 05558119
• 负责人: MOCHIZUKI Seiichi
• 金额: $ 4.1万
• 依托单位: Kawasaki College of Allied Health Professions
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05558119/
• 关键词: Microdialysis; Bioactive Compounds; Percent Recovery; Kidney; Heart; High Performance Liquid Chromatography; 中空糸膜; ふるい分け係数; EDRF

We measured the hydraulic permeability and separation characteristics of polyethersulfone membranes before and after adsorption and deposition of plasma proteins and then performed quantitative analysis of membrane performance. The hydraulic permeability of a membrane of 100,000 molecular weight cut off (MWCO) declined 20 to 30% after adsorption of bovine serum albumin. The observed sieving coefficient, which is the ratio of filtrate to bulk concentrations, of polydisperse dextran through a 30,000 MWCO membrane decreased up to 50% after albumin adsorption.According to the above results, the effects of plasma protein adsorption and deposition on membrane separation performance were significant, and it is crucial for quantitative microdialy methodology to make precise evaluation of recovery rate and % recovery before, during and after microdialysis.In the second year, we attempted to recover bioactive compounds from some organs such as kidney and heart using a 20,000 MWCO polycarbonate microdialysis probe. In our preliminary experiments, % recovery of adenosine was about 15%, and one of angiotensin II was about 7%. We successfully recovered angiotensin II of the order of ppm from a canine kidney. However, some change in membrane performance seemingly due to protein adsorption was observed, and thus, some pretreatment and/or other membrane materials may need to be considered. In another preliminary experiments of a rat heart using a Langendorff perfusion system, the perfusate from the pulmonary artery was collected, and concentrations of adenosine, inosine, and hypoxanthine were measured using a high performance liquid chromatography. We confirmed continuous monitoring of enhanced production of adenosine and its metabolites during ischemia. We are currently attempting to collect a regional myocardial fluid, especially ATP metabolites using a microdialysis technique.

测定了聚醚砜膜在吸附和沉积血浆蛋白前后的渗透性能和分离性能，并对膜性能进行了定量分析。截留分子量为100，000的膜在吸附牛血清白蛋白后，其水力渗透性下降20 - 30%。通过30，000 MWCO膜的多分散右旋糖酐的筛分系数，即滤液与本体浓度的比值，在白蛋白吸附后下降了50%。根据上述结果，血浆蛋白吸附和沉积对膜分离性能的影响是显著的，准确评价微透析前、中、后的回收率和%回收率是定量微透析方法学的关键。第二年，我们尝试使用20，000 MWCO聚碳酸酯微透析探针从一些器官如肾脏和心脏中回收生物活性化合物。在我们的初步实验中，腺苷的回收率约为15%，血管紧张素II的回收率约为7%。我们成功地从犬肾中回收了ppm量级的血管紧张素II。然而，观察到膜性能的一些变化似乎是由于蛋白质吸附，因此，可能需要考虑一些预处理和/或其他膜材料。在使用Langendorff灌注系统的大鼠心脏的另一个初步实验中，收集来自肺动脉的灌注液，并使用高效液相色谱法测量腺苷、肌苷和次黄嘌呤的浓度。我们证实了在缺血期间连续监测腺苷及其代谢物的产生增强。我们目前正试图收集局部心肌液，特别是ATP代谢物使用微透析技术。

项目成果

Seiichi Mochizuki, at al.: "Sieving characteristics of albumin deposits formed during microfiltration" Journal of Colloid and Interface Science. 158. 136-145 (1993)

Seiichi Mochizuki 等人：“微滤过程中形成的白蛋白沉积物的筛分特性”《胶体与界面科学杂志》。

望月,精一: "心筋内slosh現象が冠動脈血管内皮細胞の形態に及ぼす影響" Japanese Circulation Journal. 58Supp11. 91 (1994)

Mochizuki, Seiichi：“心肌内晃动现象对冠状动脉内皮细胞形态的影响”日本循环杂志 58Supp11 (1994)。

Fukuhiro, et al.: "Adenosine release from the ischemic myocardium following coronary reperfusion" Japanese Journal of Cardiovascular Surgery. 24 Supplement. 165 (1995)

Fukuhiro 等人：“冠状动脉再灌注后缺血心肌中的腺苷释放”，日本心血管外科杂志。

Seiichi Mochizuki, et al.: "Effect of intra-myocardial slosh phenomenon on endothelial morphology of coronary artery" Japanese Circulation Journal, 58 Supplement 1. 91 (1994)

Seiichi Mochizuki 等人：“心肌内晃动现象对冠状动脉内皮形态的影响”日本循环杂志，58 增刊 1. 91 (1994)

望月精一 他: "微細NOセンサーによる摘出イヌ大腿動脈壁中のNOの測定" Japanese Circulation Journal. 59. 389 (1995)

Seiichi Mochizuki 等人：“使用微小 NO 传感器测量离体犬股动脉壁中的 NO”，日本循环杂志 59. 389 (1995)。