Mechanisms of aluminum induced growth inhibition based on the inhibitory effects of aluminum on sugar transport in plants

基于铝对植物糖转运的抑制作用探讨铝诱导生长抑制的机制

• 批准号: 17380049
• 负责人: YAMAMOTO Yoko
• 金额: $ 10.16万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380049/
• 关键词: aluminum toxicity; cell elongation; sugar metabolism; photosynthesis; vacuole; reactive oxygen species; aluminum-activated malate transporter; germination; アルミニウム; 根伸長阻害; 糖転送; 有機酸; 細胞死; 細胞伸長阻害; ショ糖欠乏; ショ糖輸送; 植物生育阻害

A major factor to inhibit crop production in acidic soils is aluminum (Al) ion. Thus the elucidation of mechanisms of Al toxicity and tolerance will be expected to provide basic information necessary for breeding Al-tolerant crops. In this study, these mechanisms were investigated in both cultured cell system and whole plant systems, focusing on sugar metabolism which is necessary for cell elongation and root growth. The findings are as follows:1. Aluminum ion inhibits sugar uptake and stimulates sugar consumption in tobacco cells, which could lead to an increase in water potential and then the inhibition of cell elongation.2. In a whole plant system, the translocation of photoassimilates is stimulated towards the root under Al stress.3. The Al-induced cell death process is different from the sugar-starvation-induced cell death, and involves the production of reactive oxygen species, vacuolar collapse and changes of cytosolic calcium concentration in tobacco cells.4. Another physiological meaning of the Al-triggered malate secretion in wheat is to maintain the growth capability of root under Al stress. The repetitive sequences in the upstream of the ALMT1 gene, encoding the Al-activated malate transporter, seem to control the expression level of the gene. The membrane topology of ALMT1 protein was elucidated.5. Isolation and characterization of Al-sensitive mutants of rice at germination stage were performed.These results indicate novel Al symptoms related to energy metabolism. Molecular and genetical aspects of these symptoms will be studied, and the roles of Al will be elucidated in the sugar metabolism related to the organic acid secretion to support Al tolerance and the sugar metabolism related to germination.

抑制酸性土壤作物产量的主要因素是铝（Al）离子。因此，有望阐明Al毒性和耐受性的机制，将提供繁殖质量作物所必需的基本信息。在这项研究中，这些机制均在培养的细胞系统和整个植物系统中进行了研究，重点是糖代谢，这对于细胞伸长和根生长是必需的。发现如下：1。铝离子抑制糖摄取并刺激烟草细胞中的糖消耗，这可能导致水潜力增加，然后抑制细胞延长。2。在整个植物系统中，在Al应力下刺激了光鼠的易位。3。 AL诱导的细胞死亡过程与糖饥饿诱导的细胞死亡不同，涉及活性氧，液泡塌陷和烟草细胞中胞质钙浓度的变化。4。小麦触发的苹果酸元素分泌的另一个生理含义是维持根部的生长能力。编码ALMT1基因上游的重复序列，编码AL元素转运蛋白，似乎控制了基因的表达水平。阐明了Almt1蛋白的膜拓扑。5。在发芽阶段进行了水稻敏感突变体的分离和表征。这些结果表明与能量代谢有关的新症状。将研究这些症状的分子和遗传方面，AL的作用将在与有机酸分泌有关的糖代谢中阐明，以支持Al耐受性和与发芽有关的糖代谢。

项目成果

Diversity of aluminum toxicity symptoms and molecular mechanisms of aluminum tolerance in crops.

作物铝毒症状的多样性及铝耐受性的分子机制。

• 发表时间: 2005
• 期刊: Chemistry and Biology 43
• 作者: Sasaki;T.;et al.
• 通讯作者: et al.

コムギALMT1トランスポーターの膜配向性の解析

小麦 ALMT1 转运蛋白的膜取向分析

• 发表时间: 2007
• 作者: Sasaki;T.;佐々木孝行;元田弘敏
• 通讯作者: 元田弘敏

Aluminum-activated malate transporter in plants.

植物中铝激活的苹果酸转运蛋白。

• 发表时间: 2007
• 作者: Takayuki Sasaki;Yamamoto Yamamoto.
• 通讯作者: Yamamoto Yamamoto.

Inhibition of sucrose uptake as a primary toxic event caused by aluminum in tobacco cells. In : Plant nutrition for food security, human health and environmental protection (Eds, Li, C.J. et al.)

抑制蔗糖摄取是烟草细胞中铝引起的主要毒性事件。

• 发表时间: 2005
• 作者: Yoko Yamamoto;et al.
• 通讯作者: et al.

Aluminum-induced alterations of cytosolic calcium concentration and its correlation with other aluminum responses in cultured tobacco cells.

铝诱导的细胞质钙浓度的变化及其与培养烟草细胞中其他铝反应的相关性。

• 发表时间: 2005
• 期刊: Plant nutrition for food security, human health and environmental protection, eds Li, C. J., et al., Tsinghua University Press, Beijing
• 作者: Tsuchiya;Y.;et al.
• 通讯作者: et al.