Mechanisms of aluminum induced growth inhibition based on the inhibitory effects of aluminum on sugar transport in plants

基于铝对植物糖转运的抑制作用探讨铝诱导生长抑制的机制

• 批准号: 17380049
• 负责人: YAMAMOTO Yoko
• 金额: $ 10.16万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380049/
• 关键词: aluminum toxicity; cell elongation; sugar metabolism; photosynthesis; vacuole; reactive oxygen species; aluminum-activated malate transporter; germination; アルミニウム; 根伸長阻害; 糖転送; 有機酸; 細胞死; 細胞伸長阻害; ショ糖欠乏; ショ糖輸送; 植物生育阻害

A major factor to inhibit crop production in acidic soils is aluminum (Al) ion. Thus the elucidation of mechanisms of Al toxicity and tolerance will be expected to provide basic information necessary for breeding Al-tolerant crops. In this study, these mechanisms were investigated in both cultured cell system and whole plant systems, focusing on sugar metabolism which is necessary for cell elongation and root growth. The findings are as follows:1. Aluminum ion inhibits sugar uptake and stimulates sugar consumption in tobacco cells, which could lead to an increase in water potential and then the inhibition of cell elongation.2. In a whole plant system, the translocation of photoassimilates is stimulated towards the root under Al stress.3. The Al-induced cell death process is different from the sugar-starvation-induced cell death, and involves the production of reactive oxygen species, vacuolar collapse and changes of cytosolic calcium concentration in tobacco cells.4. Another physiological meaning of the Al-triggered malate secretion in wheat is to maintain the growth capability of root under Al stress. The repetitive sequences in the upstream of the ALMT1 gene, encoding the Al-activated malate transporter, seem to control the expression level of the gene. The membrane topology of ALMT1 protein was elucidated.5. Isolation and characterization of Al-sensitive mutants of rice at germination stage were performed.These results indicate novel Al symptoms related to energy metabolism. Molecular and genetical aspects of these symptoms will be studied, and the roles of Al will be elucidated in the sugar metabolism related to the organic acid secretion to support Al tolerance and the sugar metabolism related to germination.

在酸性土壤中抑制作物产量的一个主要因素是铝离子。因此，铝毒害和耐性机制的阐明有望为耐铝作物育种提供必要的基础信息。在这项研究中，这些机制在培养细胞系统和整个植物系统中都进行了研究，重点是细胞伸长和根生长所必需的糖代谢。研究结果如下：1.铝离子抑制烟草细胞对糖的吸收，刺激糖的消耗，从而导致水势升高，进而抑制细胞伸长。在整个植物系统中，铝胁迫刺激了光同化物向根的运输。铝诱导的细胞死亡过程不同于糖饥饿诱导的细胞死亡，它涉及烟草细胞内活性氧的产生、液泡的塌陷和细胞内钙离子浓度的变化。铝诱导小麦苹果酸分泌的另一个生理意义是维持根在铝胁迫下的生长能力。ALMT1基因上游的重复序列编码铝激活的苹果酸转运体，似乎控制着该基因的表达水平。阐明了ALMT1蛋白的膜拓扑结构。对水稻发芽期对铝敏感的突变体进行了分离和鉴定，这些结果表明新的铝症状与能量代谢有关。这些症状的分子和遗传学方面的研究将被阐明，铝在与有机酸分泌有关的糖代谢中的作用将被阐明，以支持铝耐性和与萌发有关的糖代谢。

项目成果

Aluminum-induced alterations of cytosolic calcium concentration and its correlation with other aluminum responses in cultured tobacco cells.

铝诱导的细胞质钙浓度的变化及其与培养烟草细胞中其他铝反应的相关性。

• 发表时间: 2005
• 期刊: Plant nutrition for food security, human health and environmental protection, eds Li, C. J., et al., Tsinghua University Press, Beijing
• 作者: Tsuchiya;Y.;et al.
• 通讯作者: et al.

イネの発芽期におけるアルミニウム耐性形質の解析

水稻发芽期耐铝性状分析

• 发表时间: 2008
• 作者: Sasaki;T.;et al.;山本洋子;古市卓也;山本洋子
• 通讯作者: 山本洋子

コムギALMTIのアルミニウム活性化機構の解析

小麦ALMTI铝活化机理分析

• 发表时间: 2007
• 作者: Sasaki;T.;佐々木孝行;元田弘敏;山本洋子;菊井聖士
• 通讯作者: 菊井聖士

Inhibition of sucrose uptake as a primary toxic event caused by aluminum in tobacco cells. In : Plant nutrition for food security, human health and environmental protection (Eds, Li, C.J. et al.)

抑制蔗糖摄取是烟草细胞中铝引起的主要毒性事件。

• 发表时间: 2005
• 作者: Yoko Yamamoto;et al.
• 通讯作者: et al.

Aluminum-activated malate transporter in plants.

植物中铝激活的苹果酸转运蛋白。

• 发表时间: 2007
• 作者: Takayuki Sasaki;Yamamoto Yamamoto.
• 通讯作者: Yamamoto Yamamoto.