Connection analysis of corticostriatal cells

皮质纹状体细胞连接分析

基本信息

批准号：
15300110
负责人：
KAWAGUCHI Yasuo
金额：
$ 10.05万
依托单位：
National Institute for Physiological Sciences (2004-2005)Okazaki National Research Institutes (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

To reveal the intracortical circuitry of generating cortical outputs to the striatum, we identified neuron types in the frontal cortex with many corticostriatal cells, and analyzed their morphologies and synaptic connections quantitatively.1.Morphological and physiological identification of pyramidal cells projecting the striatum.By fluorescent retrograde labeling we found that pyramidal cells projecting to the pontine nuclei [corticopontine (CPn) cells] and the contralateral striatum [crossed corticostriatal (CCS) cells] were separate populations, and that doublet firing at the initial phase in response to depolarization was found only in CPn cells. The apical tufts developed more in CPn cells than CCS cells, but the tuft structures were variable among CCS cells, dependent on the soma location.2.Objective identification of nonpyramidal cell subtypes and quantitative analysis of their morphologies.We identified nonpyramidal cells subtypes quantitatively from the firing and axonal arbor … More izing patterns, and analyzed their axonal branching and synaptic bouton formation as well as the dendritic branching and spine distribution quantitatively. The inter-branch and synaptic bouton distances were determined independently, and each subtypes had a unique combination of these means. Nonpyramidal cells were divided into three dendritic patterns by the primary dendrite number issuing from a soma, their extending direction, mean branch length and spine density. The spine density was highest in Martinotti cells. Composition of morphological spine types was also different among subtypes.3.Synaptic connections among identified pyramidal and nonpyramidal cell subtypes.We investigated the synaptic connections patterns among cells identified as above, using paired recording in vitro slice preparation and morphological reconstruction of intracellular stained cells. The synaptic currents from fast-spiking or CCS cells to CCS cells were compared. The current amplitude was dependent on the number of contact sites in excitatory connections, but strongly dependent on the contact locations in inhibitory connections. Less

To reveal the intracortical circuitry of generating cortical outputs to the striatum, we identified neuron types in the frontal cortex with many corticostriatal cells, and analyzed their morphology and synaptic connections quantitatively.1.Morphological and physical identification of pyramidal cells projecting the striatum.By fluorescent retrograde labeling we found that pyramidal cells projecting to the pontine nucleari [Corticopontine（CPN）细胞]和对侧纹状体[交叉皮质纹状体（CCS）细胞]是单独的群体，并且仅在CPN细胞中发现了在初始阶段对沉积的双重发射。与CCS细胞相比，在CPN细胞中，顶簇在CPN细胞中发展得更多，但是CCS细胞之间的簇结构是可变的，取决于soma位置。2。目标鉴定非锥状细胞亚型，以及对形态的定量分析，我们确定了非基础细胞的定量和轴突分析的临界量和轴突型的ARBOR ARBOR的差异型，并鉴定出数量。突触式布顿的形成以及树突分支和脊柱分布定量。分支间和突触的胸部距离是独立确定的，每个亚型都具有这些手段的独特组合。从soma发出的主要树突数，其延伸方向，平均分支长度和脊柱密度，将非锥体细胞分为三个树突状模式。马提尼细胞中的脊柱密度最高。在亚型之间，形态学脊柱类型的组成也有所不同。3。已鉴定的锥体和非锥体细胞亚型之间的突触连接。我们研究了使用成对的体外切片制备和细胞内细胞内膜内部细胞内膜的形态重建的细胞之间的合成连接模式。比较了从快速加速器或CCS细胞到CCS细胞的突触电流。当前的放大器取决于兴奋连接中的接触位点的数量，但强烈取决于抑制连接中的接触位置。较少的