Cortical GABAergic Cell Subtypes and Their Synaptic Connections

皮质 GABA 能细胞亚型及其突触连接

基本信息

批准号：
09480235
负责人：
KAWAGUCHI Yasuo
金额：
$ 7.68万
依托单位：
OKAZAKI NATIONAL RESEARCH INSTITUTES (1999)The Institute of Physical and Chemical Research (1997-1998)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Cortical GABAergic Cell Subtypes and Their Synaptic ConnectionsPhysiological, morphological immunohistochemical and pharmacological characteristics of GABAergic cells in rat frontal cortex of rats were studied in vitro. (1) Physiological and chemical heterogeneity of large GABAergic basket cells. Cholecystokinin (CCK) cells and parvalbumin cells made boutons apposed to other cell bodies. Parvalbumin cells belonged to fast-spiking (FS) cells. In contrast, CCK cells were found to be regular-spiking (RS) or burst-spiking (BS) nonpyramidal (NP) cells. Large somatostatin cells belonged to the RSNP category which did not seem to be apposed to the somata so frequently as large CCK and parvalbumin cells. (2) Selective cholinergic modulation of cortical GABAergic cell subtypes. Cholinergic or muscarinic agonists indeed induced GABAergic postsynaptic currents in pyramidal cells by exciting inhibitory interneurons. Carbachol or muscarine affected the activities of peptide-containing GABAergic cel … More ls, but not of GABAergic cells of fast-spiking characteristics containing parvalbumin and GABAergic cells of late-spiking characteristics. Somatostatin or VIP cells were depolarized with spike firing. CCK cells were affected heterogeneously by cholinergic agonists. Larger CCK cells were hyperpolarized followed by a slow depolarization, whereas smaller CCK cells were only depolarized. (3) Noradrenergic excitation and inhibition of GABAergic cell types. Noradrenaline (NA) and an a-adrenergic agonist, 6 fluoronorepinephrine (FNE) induced an increase of IPSC frequency in pyramidal cells, but not a b-adrenergic agonist. This increase was reduced by tetrodotoxin, bicuculline and a-adrenergic antagonists, suggesting that GABAergic cells are excited via a-adrenoceptors. Fast spiking or late spiking cells were depolarized by application of NA or FNE, but none demonstrated spike firings. Most somatostatin cells were depolarized, accompanied by spike firing. In a few cases this was preceded by hyperpolarization. Cholecystokinin cells were affected heterogeneously: depolarization, hyperpolarization followed by depolarization, or hyperpolarization resulted. Less

在体外，研究了皮质GABA能细胞亚型及其突触性连接，形态学免疫组织化学和药物特征的大鼠大鼠额叶皮层的体外研究。（1）大型GABA能细胞的生理和化学异质性。胆囊动蛋白（CCK）细胞和白蛋白细胞使胸子应用于其他细胞体。白蛋白细胞属于快速加速器（FS）细胞。相比之下，发现CCK细胞是规则的（RS）或爆发（BS）非锥体（NP）细胞。大型生长抑素细胞属于RSNP类别，似乎并不像大型CCK和白细胞蛋白细胞一样频繁地应用于SOMATA。（2）皮质GABA能细胞亚型的选择性胆碱能调节。胆碱能或毒蕈碱激动剂确实通过令人兴奋的抑制性神经元在金字塔细胞中诱导了锥体后的gaba能后电流。卡尔巴醇或毒arine影响了含有肽的Gabaergic Cel的活性……更多，但没有含有含有白细胞蛋白和GABA能细胞的快速刺激性特征的GABA能细胞的活性。生长抑素或VIP细胞用尖峰发射去极化。 CCK细胞受到胆碱能激动剂的异质影响。较大的CCK细胞被过极化，然后慢慢去极化，而仅部署了较小的CCK细胞。（3）去甲肾上腺素（Na）和A-肾上腺素能激动剂，6个氟甲肾上腺素（FNE）诱导金字塔细胞中IPSC频率的增加，但没有B-肾上腺素能激动剂。四毒素，双瓜氨酸和A-肾上腺素能的拮抗剂降低了这种增加，这表明GABA能细胞通过A-肾上腺肾上腺受体激发。通过应用Na或FNE部署了快速尖峰或晚期尖峰细胞，但没有一个显示尖峰射击。大多数生长抑素细胞都是通过尖峰射击来完成的。在少数情况下，这是在超极化之前。胆囊动蛋白细胞受到异质的影响：沉积，超极化，然后去极化或产生超极化。较少的