Self oscillatory growth of ice crystal of ice in the antifreeze protein solution and its dynamics

冰晶在抗冻蛋白溶液中的自振荡生长及其动力学

• 批准号: 15340136
• 负责人: FURUKAWA Yoshinori
• 金额: $ 10.37万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15340136/
• 关键词: Ice crystal; Antifreeze glycoprotein; Crystal growth kinetics; Self-oscillatory; Interface adsorption; Protein conformation; Kinetic loop; Fluorescence; 結晶成長; 不凍タンパク質; カイネティクス; 蛍光観察; 拡散場; 界面成長ステップ; 結晶成長機構; 結晶成長制御

Several kinds of ice crystal growth experiments were conducted in a solution of antifreeze glycoprotein molecules which were labeled by fluorescent material. One directional growth of ice clearly showed the oscillation of growth rates and periodic incorporation of AFGP molecules depending on the growth rate. This is the first direct observation of striation formation inside the crystal. Free growth experiments of ice single crystals also showed the oscillations of growth rates. The growth mode was ON-OFF type oscillation for the prismatic plane, whereas the continuous oscillation was observed for the basal plane.Secondary structures (conformations) of AFGP molecules under the adsorbed state at ice/water interface were examined using Attenuated Total Reflection type FTIR technique. The FTIR signal indicated that the AFGP molecules with a-helix conformation should be dominant at the ice/water interface. Since the dominant conformations of molecules in the solution showed the random state, this result means that the conformation change occur in order to strongly adsorb to the interface.A model to explain the oscillatory growth of ice crystal was also developed on the basis of the concept of kinetic loop, which was derived from the hysteresis relationships between the growth rate and the driving force. This model also suggest that the AFGP molecule adsorption on the interface should occur in two states, namely week adsorption state and tight adsorption state, which is consistent with the experimental result obtained by ATR-FTIR measurement.As a result, we clarified the dynamics of self-oscillatory growth of ice crystal in the AFGP solution. Understanding the crystal growth dynamics strongly affected by the adsorption of AFGP molecules will play an important role to clarify the antifreeze function of these proteins.

在用荧光材料标记的抗冻糖蛋白分子溶液中进行了几种冰晶生长实验。冰的单向生长清楚地显示出生长速度的振荡和AFGP分子的周期性掺入，取决于生长速度。这是第一次在晶体内部直接观察到条纹的形成。冰单晶体的自由生长实验也显示出生长速率的振荡。利用衰减全反射型FTIR技术研究了AFGP分子在冰/水界面吸附状态下的二级结构(构象)。FTIR信号表明，AFGP分子在冰/水界面上以a-螺旋构象为主。由于溶液中分子的主要构象呈现随机状态，这一结果意味着分子的构象变化是为了强吸附到界面上。基于动力学环的概念，从生长速度与驱动力之间的滞后关系出发，建立了冰晶振荡生长的模型。该模型还提出AFGP分子在界面上的吸附应处于弱吸附和紧密吸附两种状态，这与ATR-FTIR实验结果一致，从而阐明了AFGP溶液中冰晶自振荡生长的动力学。了解AFGP分子吸附对晶体生长动力学的强烈影响，对于阐明这些蛋白质的抗冻功能具有重要意义。

项目成果

氷点下でも凍らない魚-不凍タンパク質が氷の結晶成長を支配する-

即使在零下温度下也不会结冰的鱼 - 抗冻蛋白控制冰晶生长 -

• 发表时间: 2005
• 期刊: バイオニクス 3
• 作者: H.NADA;J.P.VAN DER EERDEN;Y.FURUKAWA;灘 裕樹;灘 浩樹;灘 浩樹;古川 義純
• 通讯作者: 古川 義純

Morphology instability on an ice crystal growing in supercooled water.

过冷水中生长的冰晶的形态不稳定性。

• 发表时间: 2005
• 期刊: Current Topic in Crystal Growth(ed.Y.Hayakawa and Y.Furukawa)(Resarch Trends, India),
• 作者: Y.Furukawa;E.Yokoyama;W.Shimada
• 通讯作者: W.Shimada

Growth kinetics on interface between(2021)plane of ice and water investifated by a molecular dynamics simulation.

通过分子动力学模拟研究冰和水（2021）平面之间界面的生长动力学。

• 发表时间: 2007
• 期刊: Proceedings on International Conference on Physics and Chemistry of Ice, 印刷中
• 作者: H.Nada;Y.Furukawa
• 通讯作者: Y.Furukawa

Diffusion, incorporation, and segregation of antifreeze proteins at the ice/solution interface.

抗冻蛋白在冰/溶液界面的扩散、掺入和分离。

• 发表时间: 2007
• 期刊: Proceedings on International Conference on Physics and Chemistry of Ice
• 作者: S.Zepeda;H.Nakaya;Y.Uda;E.Yokoyama;Y.Furukawa
• 通讯作者: Y.Furukawa

In situ observation of crystal growth under microgravity condition

微重力条件下晶体生长的原位观察

• 发表时间: 2005
• 期刊: J. Visualization Soc. of Jpn. 25
• 作者: K.Tsukamoto;Y.Nishimura;E.Yokoyama
• 通讯作者: E.Yokoyama