Development and Application of High Performance Capillary Electrophoresis Using a New Capillary Fabricated by Laser

激光制作的新型毛细管高性能毛细管电泳的开发与应用

• 批准号: 15350045
• 负责人: KANETA Takashi
• 金额: $ 6.85万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15350045/
• 关键词: Laser-fabricated capillary; Capillary electrophoresis; Hadamard transformation; Electrokinetic injection; Multiple sample injection; Laser-induced fluorescence; Post-column reaction; Trace Analysis; 光学ゲート法; 蛍光検出; レーザー励起蛍光検出; タンパク質; イムノアッセイ; 光

A novel capillary fabricated by laser ablation was developed and applied to Hadamard transform capillary electrophoresis and post-column reaction for fluorescence detection in capillary electrophoresis. A small hole was fabricated at the center of the capillary. The small hole functions as an inlet port for the sample and labeling reagent in multiple sample injection and post-labeling reaction, respectively. In multiple sample injection, the hole is immersed in a sample solution and the end of the capillary that is usually employed for sample introduction is immersed in a buffer solution. A high potential is continuously applied between the injection port and the end of the capillary, which allows the sample solution to be introduced into the separation capillary. By application of a higher potential modulated according to a Hadamard code between both ends of the capillary, the buffer solution is injected into the separation capillary. This method was successfully employed for multiple sample injection in Hadamard transform capillary electrophoresis equipped with an absorbance detector and a laser-induced fluorescence detector. In post-column reaction, the hole was made at upstream of a fluorescence detector. The hole is immersed in a labeling reagent solution. Analyte zones separated by capillary electrophoresis were mixed with the labeling reagent introduced from the hole, followed by the reaction of the labeling reagent with analytes and fluorescence detection of the labeled analytes.

研制了一种新型激光烧蚀毛细管，并将其应用于阿达玛变换毛细管电泳和毛细管电泳柱后反应中荧光检测。在毛细管中心制造了一个小孔。小孔在多次进样和标记后反应中分别作为样品和标记试剂的进样口。在多次进样时，将孔浸入样品溶液中，通常用于进样的毛细管末端浸入缓冲溶液中。在进样口和毛细管末端之间连续施加高电位，使样品溶液被引入分离毛细管。通过在毛细管两端按Hadamard码调制的高电位的应用，缓冲溶液被注入分离毛细管。该方法成功地应用于Hadamard变换毛细管电泳中，并配有吸光度检测器和激光诱导荧光检测器。在柱后反应中，在荧光检测器的上游开孔。将孔浸入标记试剂溶液中。将毛细管电泳分离出的分析物带与孔中引入的标记试剂混合，标记试剂与分析物反应，对标记物进行荧光检测。

项目成果

On-Line Concentration of a Protein Using Denaturation by Sodium Dodecyl Sulfate

• DOI: 10.2116/analsci.21.37
• 发表时间: 2005-01
• 期刊: Analytical Sciences
• 影响因子: 1.6
• 作者: P. Jing;T. Kaneta;T. Imasaka

Suppression of electroosmotic flow and its application to determination of electrophoretic mobilities in a poly(vinylpyrrolidone)-coated capillary

• DOI: 10.1016/j.chroma.2005.08.062
• 发表时间: 2006-02-17
• 期刊: JOURNAL OF CHROMATOGRAPHY A
• 影响因子: 4.1
• 作者: Kaneta, T;Ueda, T;Imasaka, T
• 通讯作者: Imasaka, T

Kazuki Hata, Yasuhiko Kichise, Takashi Kaneta, Totaro Imasaka: "Hadamard Transform Microchip Electrophoresis Combined with Diode Laser Fluorometry"Analytical Chemistry. 75. 1765-1768 (2003)

Kazuki Hata、Yasuhiko Kichise、Takashi Kaneta、Totaro Imasaka：“Hadamard 变换微芯片电泳与二极管激光荧光法相结合”分析化学。

Takashi Kaneta, Kazuki Kosai, Totaro Imasaka: "Detection of 27 Molecules in A Single Injection Volume by Hadamard Transform Capillary Electrophoresis"Analytical Sciences. 19. 1659-1661 (2003)

Takashi Kaneta、Kazuki Kosai、Totaro Imasaka：“通过 Hadamard 变换毛细管电泳检测单次注射体积中的 27 个分子”分析科学。

Application of Hadamard transformation to chiral separation by capillary electrophoresis

Hadamard变换在毛细管电泳手性分离中的应用

• 发表时间: 2003
• 期刊: BUNSEKI KAGAKU 52
• 作者: Takashi Kaneta;Miki Nishida;Totaro Imasaka
• 通讯作者: Totaro Imasaka