RNA splicing: factors and mechanisms

RNA剪接：因素和机制

• 批准号: DP0344909
• 负责人: Prof Brian Morris
• 金额: $ 14.64万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-02-19 至 2006-02-18
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0344909
• 关键词: RNA; splicing; factors; mechanisms

Most primary gene transcripts must have their noncoding intronic sequences spliced out before the mRNA can be translated. Moreover, alternative splicing enables cells to generate a far more proteins than there are genes in the nucleus. Based on our proven success with ZNF265 we will isolate novel RNA interactors and their partners, colocalize these in intranuclear compartments, and elucidate their effect on pre-mRNA splicing. This will provide timely spin-offs to the Human genome Project and EST sequence information, where the finding of only approx. 30,000 genes in our genome highlights the important role of alternative splicing in generating the large proteome repertoire of cells. This will bring considerable benefits to science, society, and the biotech industry.

在mRNA被翻译之前，大多数初级基因转录物必须将它们的非编码内含子序列剪接掉。此外，选择性剪接使细胞能够产生比细胞核中基因多得多的蛋白质。基于我们在ZNF265上的成功，我们将分离出新的RNA相互作用物及其伴侣，将它们共定位在核内室中，并阐明它们对mrna前剪接的影响。这将及时为人类基因组计划和EST序列的衍生品提供信息，其中的发现只有大约。我们基因组中的30,000个基因突出了选择性剪接在产生大量细胞蛋白质组库中的重要作用。这将为科学、社会和生物技术产业带来可观的利益。