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Functional genomics of the model horticultural crop, Micro-Tom.

模型园艺作物 Micro-Tom 的功能基因组学。

基本信息

批准号：
15380019
负责人：
KANAYAMA Yoshinori
金额：
$ 10.18万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380019/
关键词：
tomato Micro-Tom fruit functional genomics 耐病性プロトンポンププロモーター

项目摘要

Functional genomics is lately developing because all DNA sequences of the genome have been clarified in Arabidopsis thaliana that is the model plant. On the other hand, functional genomics in horticultural crops is not easy because there are so many species involved in horticultural crops. Therefore, an advanced research by using some model horticultural crops is needed. Arabidopsis thaliana is not useful as the model of the fruit trees and the fruit vegetables.Tomato is not only commercially important but also useful as a model horticultural plant because its transformation is comparatively easy ; its physiological information is abundant ; there is expressed sequence tag (EST) containing more than 100,000 sequences. However, there are some problems, which are the large plant size and the long generation time. Micro-Tom is a miniature tomato that is almost genetically identical to the cultivation tomatoes. Micro-Tom could be a good model plant because of its small size (5-10 cm of plant height), short generation time, and high transformation efficiency. Thus, Micro-Tom was used in this experiment as a model horticultural crop for molecular genetics.A high degree of availability of Micro-Tom as a model experimental plant was confirmed through the analysis of proton pump, pathogen response, etc. in this research. Moreover, the research on various horticultural crops such as the fruit vegetables and fruit trees was also carried out. In the future, the information and the technique of functional genomic developed using Micro-Tom are expected to be applied widely in various horticultural crops.

功能基因组学是近年来发展起来的一门学科，其主要原因是模式植物拟南芥基因组的全部DNA序列已被明确。另一方面，由于园艺作物涉及的物种众多，因此进行功能基因组学研究并不容易。因此，有必要利用一些示范园艺作物进行进一步的研究。拟南芥不适合作为果树和水果蔬菜的模型。番茄的转化相对容易，不仅具有重要的商业价值，而且作为一种示范园艺植物也很有用；它的生理信息丰富；存在包含超过100,000个序列的表达序列标签（EST）。但也存在电厂规模大、发电时间长等问题。微型番茄是一种微型番茄，其基因与栽培番茄几乎相同。微汤姆植株体积小（株高5 ~ 10 cm），世代时间短，转化效率高，是一种很好的模式植物。因此，本试验以微汤姆为示范园艺作物进行分子遗传学研究。本研究通过对质子泵、病原菌反应等方面的分析，证实了微汤姆作为模型实验植物的高可利用性。此外，还开展了水果、蔬菜、果树等多种园艺作物的研究。在未来，利用Micro-Tom开发的信息和功能基因组技术有望在各种园艺作物中得到广泛应用。

项目成果

期刊论文数量（36）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Kaori Kikuchi: "Changes in Sugar-related Enzymes during Wilting of Cut Delphinium Flowers"Journal of the Japanese Society for Horticultural Science. 72・1. 37-42 (2003)

菊池香织：“飞燕草切花枯萎时与糖相关的酶的变化”日本园艺学会杂志72・1（2003）。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Effects of low temperature and abscisic acid on the expression of the sorbitol-6-phosphate dehydrogenase gene in apple leaves

DOI：
10.2503/jjshs.75.20
发表时间：
2006-01-01
期刊：
JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
影响因子：
0
作者：
Kanayama, Y;Watanabe, M;Yamaki, S
通讯作者：
Yamaki, S

Morphological and molecular analyses of the tomato floral mutant leafy inflorescence, a new allele of falsiflora

DOI：
10.1016/j.plantsci.2005.03.007
发表时间：
2005-07-01
期刊：
PLANT SCIENCE
影响因子：
5.2
作者：
Kato, K;Ohta, K;Kanayama, Y
通讯作者：
Kanayama, Y

Antagonisitic interaction beween the SA and JA signaling pathways in Arabidopsis modulate expression of defence genes and gene-for-gene resistance +

拟南芥中 SA 和 JA 信号通路之间的拮抗相互作用调节防御基因的表达和基因间抗性

DOI：
发表时间：
2004
期刊：
Plant and Cell Physiology 45・6
影响因子：
0
作者：
Kazuhiro Ohta et al.;Kazuhisa Kato et al.;Yoshinori Kanayama et al.;Kazuhiro Ohta et al.;Hideki Takahashi et al.;Ming Shu Zheng et al.;Takeaki Ishihara;Ken-taro Sekine;Michihito Deguchi;Hideki Takahashi;Takeaki Ishihara et al.;T.Ochiai et al.;Ken-taro Sekine et al.;P.-Y.Yun et al.;Michihito Deguchi et al.;P.-Y.Yun et al.;Hideki Takahashi et al.;J.-H.Park et al.;Hideki Takahashi
通讯作者：
Hideki Takahashi

The enhanced resistance to Cucumber mosaic virus in the Arabidopsis thaliana stearoyl-ACP desaturase-deficient ssi2 mutant is mediated by an SA-independent, but EDS5-dependent signaling mechanism

拟南芥硬脂酰-ACP去饱和酶缺陷的ssi2突变体对黄瓜花叶病毒的抵抗力增强是由不依赖SA但依赖EDS5的信号机制介导的

DOI：
发表时间：
2004
期刊：
Molecular Plant-Microbe Interaction 17・6
影响因子：
0
作者：
Kazuhiro Ohta et al.;Kazuhisa Kato et al.;Yoshinori Kanayama et al.;Kazuhiro Ohta et al.;Hideki Takahashi et al.;Ming Shu Zheng et al.;Takeaki Ishihara;Ken-taro Sekine
通讯作者：
Ken-taro Sekine