Studies on seedlessness of loquat fruit as related to endogenous plant hormone.

枇杷果实无籽与内源植物激素相关的研究

• 批准号: 15380021
• 负责人: MATSUI Hiroyuki
• 金额: $ 9.6万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380021/
• 关键词: Loquat; Breeding; Polyploid; Seedless fruit; Endogenous plant hormone; Plant growth regulator; Sugar composition; Fruit growth; 植物ホルモン; 糖含量; 成熟; 形態; アミプロフォスメチル; コルヒチン; GA_3; CPPU; ジベレリン; サイトカイニン; オーキシン; アブシジン酸; 単為結果; 着果; 生長

1. Six tetraploid loquats were obtained by applying amiprofos-methyl and colchicine for chromosome doubling agents to diploid loquat shoot apex.2. The ratios of triploid seedlings produced by reciprocal crossing between tetraploids and diploids ranged from 92% to 100%. There were no great differences among the reciprocal crosses.3. Leaf width and thickness, stomata length, maximal diameter of calyx, petal length and width, and pollen grain diameter in the dr-, tri- and tetraploid plants significantly increased with increasing polyploidy level in loquats. The pollen germination ratios were high at 95% in both the diploid and the tetraploid. However, it was markedly low at 8% in the triploid, and the fructification was also extremely low at 0.1%.4. When 1,000ppm-GA_3 plus 20ppm-CPPU was applied to flowers and fruits two times at bloom and about 60 days after bloom of triploid loquat, the percentage of fruit set at harvest was highest (92%). On the other hand, Set of fruit applied with CPPU or GA_3 alone was 31% and 53%, respectively. Fruit set and Size of seedless fruit in the applications of GA_3 plus CPPU increased linearly with increasing GA_3 concentration. However, brix decreased in the treatments including CPPU. This cause depended on decrease of sucrose content.5. GA-like substances were extracted from immature seeds of loquat. After it was fractionated by ODS-HPLC, each fraction was applied to flowers and fruits of diploid loquat. Seedless fruits were induced in treatment of five fractions, but the percentage of fruit set varied among fractions.6. When 1,000ppm-GA_3, 20ppm-CPPU, 100ppm-NAA, 1,000ppm-ABA and 400ppm-ethepnone were applied to diploid loquat fruits during 60 to 20 days before harvest, fruit size in GA_3 and CPPU treatments, and sugar content in ethephone, CPPU and GA_3 treatments increased. However, NAA application resulted in low sugar and high acid contents.

1。通过施用氨基甲基甲基和秋水仙碱将染色体双倍剂涂成二倍体loquat loquat shack Apex.2，获得了六个四倍体lo。四倍体和二倍体之间相互交叉产生的三倍体幼苗的比率为92％至100％。互惠十字之间没有很大的差异。3。叶宽度和厚度，气孔长度，花萼的最大直径，花瓣长度和宽度以及DR，三倍倍植物和四倍体植物的花粉粒直径随着Loquats的多倍体水平的增加而显着增加。在二倍体和四倍体中，花粉发芽比在95％处较高。但是，在三倍体中，它显着低，为8％，果实化也极低，为0.1％.4。当将1,000ppm-ga_3加上20ppm-cppu应用于花朵和水果两次在布鲁姆，大约60天后，大约60天后，收获的果实的百分比最高（92％）。另一方面，仅使用CPPU或GA_3应用的一组水果分别为31％和53％。在GA_3和CPPU的应用中，果实集和无种子水果的大小随着GA_3浓度的增加线性增加。但是，包括CPPU在内的治疗中的brix减少。这原因取决于蔗糖含量的减少5。从Loquat的未成熟种子中提取Ga样物质。用ODS-HPLC分馏后，将每个馏分应用于二倍体loquat的花和果实。在治疗五个分数时诱导了无籽的水果，但是水果集的百分比在分数之间有所不同。6。当1,000ppm-ga_3，20ppm-cppu，100ppm-naa，1,000ppm-aba和400ppm-ethepnone在收获前的60至20天内应用于二倍体loquat果实，GA_3和CPPU处理中的水果大小，以及eThephOne，CPPU和GA_3处理中的糖含量。但是，NAA的应用导致低糖和高酸含量。

项目成果

アミプロフォスメチルおよびコルヒチンによるビワ四倍体の作出

甲基胺丙磷和秋水仙碱生产枇杷四倍体

• 发表时间: 2004
• 期刊: 園芸学研究 3巻4号
• 作者: 八幡茂木;佐藤三郎;小原 均;松井弘之
• 通讯作者: 松井弘之

Induction of parthenocarpic fruit growth with endogenous gibberellins of loquat.

枇杷内源赤霉素诱导单性结实果实生长。

• 发表时间: 2004
• 期刊: Acta Horticulturae 653
• 作者: Sadamatu M.;K.Ohkawa;H.Ohara;H.Matsui
• 通讯作者: H.Matsui

Differences of morphological and fruit bearing characteristics among the different ploidy plants and triploid production by crossings between diploid and triploid plants in loquat.

枇杷不同倍性植株形态和结果特性的差异及二倍体与三倍体杂交产生三倍体的研究.

• 发表时间: 2005
• 期刊: Hort.Res.Japan. 4(4)
• 作者: Yahata;S. et al.
• 通讯作者: S. et al.

Effects of different concentrations of GA_3 in combination with or without CPPU on induction of seedless fruit set and development in triploid loquat.

不同浓度GA_3联合或不联合CPPU对三倍体枇杷无核坐果诱导及发育的影响

• 发表时间: 2005
• 期刊: 10^<th> International Symposium on Plant Bioregulators in Fruit Production
• 作者: Ohara H.;M.Miwa;S.Yahata;K.Ohokawa;H.Matsui
• 通讯作者: H.Matsui

ビワの無種子果実生産.

枇杷无核果品生产。

• 发表时间: 2004
• 期刊: 植物の成長調節 39巻1号
• 作者: 松井弘之;八幡茂木;佐藤三郎;小原 均;大川克哉;三輪正幸
• 通讯作者: 三輪正幸