Endogenous gibberellins in developing grape berry and induction of seedlessness berry using them

内源赤霉素在葡萄浆果发育中的作用及利用其诱导无核浆果

• 批准号: 12660019
• 负责人: MATSUI Hiroyuki
• 金额: $ 2.3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660019/
• 关键词: Grape; Gibberellin; Seedlessness; Campbell Early; Delaware; Kosyu; GA_3; GA_4; GA_7

1. Endogenous giberellins (GAs) were extracted from immature seeds of Campbell Early, Delaware and Kosyu grapes. After ODS-HPLC, the fractions that showed GAs activity in three cultivars were No.14-15 (I), No.18-19 (II) No.23-25 (III) and No.26-27 (IV). However, total GAs activity was higher in Campbell Early followed by Delaware and Kosyu. GAs present in IV of Campbell Early were identified as GA_4, 3-epi-GA_4, GA_7 and GA_<34>, by GC-MS.2. GA_3, GA_4 or GA_7 were appllied to flower cluster 2 weeks before and 10 days after full bloom. These GAs induced parthenocarpy in Campbell Early, Delaware and Kosyu grapes. However, the effect of the treated GAs was different among cultivars. Higher percentages of berry set in. Campbell Early, Delaware and Kosyu were observed in order GA_7(96%)>GA_3(71%)>GA_4(66%), GA_7(99%)>GA_4(97%)>GA_3(94%) and A_3(28%)>GA_7(16%)>GA_4(14%), respectively. And also, the aplication GA_3, GA_4 or GA_7 to Campbell Early and Delaware strongly inhibited the pollen germination, but such a trend was not observed in Kosyu.3. GAs extracted from immature seeds of Campbell Early and Kosyu were applied to the emasculated florets of both cultivars at anthesis. They induced parthenocarpy, but the percentage of berry set varied between cultivars.

1. 从Campbell Early、Delaware和Kosyu葡萄的未成熟种子中提取内源赤霉素（Endogenous giberellins, GAs）。经ODS-HPLC分析，3个品种中具有GAs活性的部位分别为14-15号(I)、18-19号（II）、23-25号（III）和26-27号（IV）。然而，Campbell Early的总GAs活性较高，其次是Delaware和Kosyu。通过气相色谱-质谱分析，鉴定出Campbell Early IV中存在的气体为GA_4、3-epi-GA_4、GA_7和GA_<34>。GA_3、GA_4和GA_7分别在花期前2周和花期后10天施用。这些气体诱导Campbell Early、Delaware和Kosyu葡萄孤雌结核。但不同品种间的处理效果不同。浆果的比例增加了。Campbell Early、Delaware和Kosyu分别为GA_7(96%)>GA_3(71%)>GA_4（66%）、GA_7(99%)>GA_4(97%)>GA_3（94%）和A_3(28%)>GA_7(16%)>GA_4（14%）。另外，施用GA_3、GA_4和GA_7对金宝宝和特拉华的花粉萌发有明显的抑制作用，但对科苏没有明显的抑制作用。从坎贝尔早期和科苏的未成熟种子中提取的气体应用于两种品种的开花时的阉割小花。它们诱导孤雌核，但果实结实率在不同品种之间存在差异。

项目成果

Matsui H. et al.: "Effect of gibberellins on induction of parthenocarpic berry growth of three grape cultivars and their endogenous gibberellins"ASEV 52nd Annual Meeting. 81 (2001)

Matsui H.等人：“赤霉素对三种葡萄品种及其内源赤霉素诱导单性结实浆果生长的影响”ASEV第52届年会。

Kato, K., H.Ohara, E.Takahashi, H.Matsui: "Endogenous gibberellin-induced parthenocarpy in grape berries"Acta Hort.. 514. 69-74 (2000)

Kato, K.、H.Ohara、E.Takahashi、H.Matsui：“葡萄浆果中内源赤霉素诱导的单性结实”Acta Hort.. 514. 69-74 (2000)

Matsui, H., T. Hori, H. Ohara and K. Ohokawa: "Effect of gibberellins on induction of Parthenocarpic berry growth of three grape cultivars and their endogenous gibberellins"ASEV 52^<nd> Annual Meeting. 81 (2001)

Matsui, H., T. Hori, H. Ohara 和 K. Ohokawa：“赤霉素对三种葡萄品种及其内源赤霉素诱导单性结实浆果生长的影响”ASEV 52^<nd>年会。

Matsui, H., T.Hori, H.Ohara, K.Ohokawa: "Effect of gibberellins on induction of parthenocarpic berry growth of three grape cultivars and their endogenous gibberellins"ASEV 52^<ed> Annual Meeting. 81 (2001)

Matsui, H., T.Hori, H.Ohara, K.Ohokawa：“赤霉素对三种葡萄品种及其内源赤霉素诱导单性结实浆果生长的影响”ASEV 52^<ed>年会。

Kato, K., H. Ohara, E. Takahashi and H. Matsui: "Endogenous gibberellin-induced parthenocarpy in grape berries"Acta Hort.. 514. 69-74 (2000)

Kato, K.、H. Ohara、E. Takahashi 和 H. Matsui：“葡萄浆果中内源赤霉素诱导的单性结实”Acta Hort.. 514. 69-74 (2000)