Endogenous gibberellins in developing grape berry and induction of seedlessness berry using them

内源赤霉素在葡萄浆果发育中的作用及利用其诱导无核浆果

• 批准号: 12660019
• 负责人: MATSUI Hiroyuki
• 金额: $ 2.3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660019/
• 关键词: Grape; Gibberellin; Seedlessness; Campbell Early; Delaware; Kosyu; GA_3; GA_4; GA_7

1. Endogenous giberellins (GAs) were extracted from immature seeds of Campbell Early, Delaware and Kosyu grapes. After ODS-HPLC, the fractions that showed GAs activity in three cultivars were No.14-15 (I), No.18-19 (II) No.23-25 (III) and No.26-27 (IV). However, total GAs activity was higher in Campbell Early followed by Delaware and Kosyu. GAs present in IV of Campbell Early were identified as GA_4, 3-epi-GA_4, GA_7 and GA_<34>, by GC-MS.2. GA_3, GA_4 or GA_7 were appllied to flower cluster 2 weeks before and 10 days after full bloom. These GAs induced parthenocarpy in Campbell Early, Delaware and Kosyu grapes. However, the effect of the treated GAs was different among cultivars. Higher percentages of berry set in. Campbell Early, Delaware and Kosyu were observed in order GA_7(96%)>GA_3(71%)>GA_4(66%), GA_7(99%)>GA_4(97%)>GA_3(94%) and A_3(28%)>GA_7(16%)>GA_4(14%), respectively. And also, the aplication GA_3, GA_4 or GA_7 to Campbell Early and Delaware strongly inhibited the pollen germination, but such a trend was not observed in Kosyu.3. GAs extracted from immature seeds of Campbell Early and Kosyu were applied to the emasculated florets of both cultivars at anthesis. They induced parthenocarpy, but the percentage of berry set varied between cultivars.

1.从坎贝尔早期、特拉华州和Kosyu葡萄的未成熟种子中提取内源赤霉素（GAs）。经ODS-HPLC分析，3个品种中表现出GAs活性的组分依次为14 -15（Ⅰ）、18 -19（Ⅱ）、23 -25（Ⅲ）和26 -27（Ⅳ）。然而，总GAs活性较高，在坎贝尔早期，其次是特拉华州和Kosyu。经GC-MS分析，坎贝尔早期IV中的GA分别为GA_4、3-epi-GA_4、GA_7和GA_<34>1。GA_3、GA_4或GA_7分别在花前2周和盛花后10天对花簇进行处理。这些GAs诱导坎贝尔早期，特拉华州和Kosyu葡萄单性结实。但不同品种的GAs处理效果不同。浆果的比例更高。坎贝尔早、特拉华州和科休的遗传多样性依次为GA_7（96%）&gt;GA_3（71%）&gt;GA_4（66%）、GA_7（99%）&gt;GA_4（97%）&gt;GA_3（94%）和A_3（28%）&gt;GA_7（16%）&gt;GA_4（14%）。GA_3、GA_4和GA_7对坎贝尔早、特拉华州的花粉萌发有明显的抑制作用，但对高雪没有这种抑制作用.从坎贝尔早期和Kosyu的未成熟种子中提取的GAs被施加到这两个品种在开花期的去雄小花上。它们诱导单性结实，但浆果座率因品种而异。

项目成果

Matsui H. et al.: "Effect of gibberellins on induction of parthenocarpic berry growth of three grape cultivars and their endogenous gibberellins"ASEV 52nd Annual Meeting. 81 (2001)

Matsui H.等人：“赤霉素对三种葡萄品种及其内源赤霉素诱导单性结实浆果生长的影响”ASEV第52届年会。

Kato, K., H.Ohara, E.Takahashi, H.Matsui: "Endogenous gibberellin-induced parthenocarpy in grape berries"Acta Hort.. 514. 69-74 (2000)

Kato, K.、H.Ohara、E.Takahashi、H.Matsui：“葡萄浆果中内源赤霉素诱导的单性结实”Acta Hort.. 514. 69-74 (2000)

Matsui, H., T.Hori, H.Ohara, K.Ohokawa: "Effect of gibberellins on induction of parthenocarpic berry growth of three grape cultivars and their endogenous gibberellins"ASEV 52^<ed> Annual Meeting. 81 (2001)

Matsui, H., T.Hori, H.Ohara, K.Ohokawa：“赤霉素对三种葡萄品种及其内源赤霉素诱导单性结实浆果生长的影响”ASEV 52^<ed>年会。

Matsui, H., T. Hori, H. Ohara and K. Ohokawa: "Effect of gibberellins on induction of Parthenocarpic berry growth of three grape cultivars and their endogenous gibberellins"ASEV 52^<nd> Annual Meeting. 81 (2001)

Matsui, H., T. Hori, H. Ohara 和 K. Ohokawa：“赤霉素对三种葡萄品种及其内源赤霉素诱导单性结实浆果生长的影响”ASEV 52^<nd>年会。

Kato, K., H. Ohara, E. Takahashi and H. Matsui: "Endogenous gibberellin-induced parthenocarpy in grape berries"Acta Hort.. 514. 69-74 (2000)

Kato, K.、H. Ohara、E. Takahashi 和 H. Matsui：“葡萄浆果中内源赤霉素诱导的单性结实”Acta Hort.. 514. 69-74 (2000)