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Fundamental study on technological development for sustainable agriculture using halophytes

盐生植物可持续农业技术开发基础研究

基本信息

批准号：
15380225
负责人：
YAMAZAKI Sunao
金额：
$ 8.77万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In order clarify the salt-tolerant mechanism of halophytes, the research was focused on the physiological and biochemical characteristics of the most common osmoprotectant, glycine betaine (GB). The results were summarized below.1)Salt-tolerant species accumulates GB in seeds.Germinating process of the seeds is the most salt-stress- acceptable period. How to clear the salt effect during germination was studied and we clarified that salt-tolerant species accumulates GB in seeds in a fashion dependent on the salt-tolerance. The amount of GB accumulated was correspondent to the strength to salt-tolerance of the species.2)Life cycle of halophytes under salt-stress.The salt-stress during germination was overcome by inherent GB in seeds. After germination the plant produced GB by itself. During growth GB accelerated the photosynthetic ability by producing a photosynthetic protein, plastocyanin. During maturation the plant accumulates GB in seeds for its next generation. It was found that the … More plant adapts itself to the environmental stresses by controlling the organic molecule like GB.3)Difference in salt-tolerance among barley varieties.About 340 variety species of barley seedlings were kindly supplied from Germplasm Center, Okayama University. Among them, 14 species were already known the salt-tolerance. The GB content of the 14 species was analyzed by capillary electrophoresis which we developed. The result was clearly shown that the GB content was proportional to the strength to salt-stress. It was also found that the present method was quite suitable for selecting the salt-tolerant species among more than ten thousands of barley varieties.4)Characterization of proteins induced by GB treatment under salt-stress.Common bean was cultured hydroponically under salt-srtess condition and GB was added to the medium. After incubation proteins in the leaves were analyzed by 2-dimesional gel electrophoresis. One of the newly appeared spots was analyzed by maldi-tof-MS and searched the sequences by protein database. Plastocyanin was found to be induced by the presence of GB. The other proteins composing thylakoid membrane was under investigation. Less

为了阐明盐生植物的耐盐机理，对盐生植物中最常用的植物保护剂甜菜碱（GB）的生理生化特性进行了研究。结果表明：1）耐盐植物在种子中积累GB，种子萌发过程是最易接受盐胁迫的时期。研究了种子萌发过程中盐效应的消除方法，阐明了耐盐植物种子中GB的积累与其耐盐性有关。盐胁迫下盐生植物的生活史：种子萌发过程中的盐胁迫是由种子中固有的GB克服的。发芽后，植物本身产生GB。在生长过程中，GB通过产生光合蛋白质质体蓝素来促进光合能力。在成熟过程中，植物在种子中积累GB以供下一代生长。发现该 ...更多信息大麦品种间的耐盐性差异以冈山大学种质资源中心提供的340个品种的大麦幼苗为材料，研究了大麦品种间的耐盐性差异。其中14种植物已被鉴定为耐盐植物。采用自行研制的毛细管电泳法对14种植物的GB含量进行了分析。结果表明，GB含量与盐胁迫强度成正比。4）盐胁迫下GB诱导的蛋白质的特性研究在无盐条件下水培菜豆，在培养基中添加GB，并对GB诱导的蛋白质进行了分析。孵育后，通过二维凝胶电泳分析叶片中的蛋白质。对其中一个新出现的斑点进行了MALDI-TOF-MS分析，并在蛋白质数据库中进行了序列检索。体蓝素被发现诱导GB的存在。其他组成类囊体膜的蛋白质正在研究中。少