Construction of Micro Bioanalytical Chemistry Lab,Systems and Single Cellular Biochemistry Integrated on a Glass Chip

玻璃芯片上集成的微量生物分析化学实验室、系统和单细胞生物化学的构建

• 批准号: 11794006
• 负责人: YAMAZAKI Sunao
• 金额: --
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11794006/
• 关键词: Labs-on-a-chip; microchip; micro-total analysis systems; ion pair extraction; immunoassay; solvent extraction; heavy ion analysis; single cell detection; 単一細胞分析; 熱レンズ顕微鏡; 集積化学実験室; μTAS; マイクロ・トータル・アナリシス・システム; 免疫分析

We have developed fundamental unit operations essential to integration of biochemical analysis, collaborating with the research project from Kanagawa Academy of Science and Technology (KAST).The integrated chemical systems have desirable characteristics, such as reduction in reagent consumption, waste and analysis time. Taking these advantages, we have demonstrated many applications, including solvent extraction, flow-injection analysis, laser reaction control, enzymatic reaction and immunoassay. We also developed ultra sensitive detection device, thermal lens microscope and apply this to single-cell imaging.The microspace in the microchannel is characterized by the large specific interfacial area and short diffusion distance, and these characteristics may contribute to highly efficient extraction and mixing with much shorter time than general method. The integration of wet analysis of heavy metal ions such as cobalt and iron leads to a considerable reduction in analyzing time. The tim … More e for extraction in the integrated system was about tenfold shorter than a conventional system using a separately funnel and mechanical shaker. Moreover, troublesome operations could be omitted.We have also integrated the immunoassay system using solid surface. This integration reduced the time necessary for the overall CEA analysis from 2d to 35min. Moreover, when serum samples were assayed with this system, there was a high correlation with the conventional ELISA. These results showed the applicability of integrated analytical systems.Thermal lens microscope could detect nonfluorescent biological substances with extremely high sensitivity without and labeling materials and had a high spatial resolution of 〜1_m. This system was applied to monitoring of cytochrome c distribution in a neuroblastoma-glioma hybrid cell cultured in the microflask fabricated in a glass microchip. Cytochrome c release from mitochondria to cytosol during the apoptosis process was successfully monitored with this system.The results of this research will bring very welcome developments to biochemical studies. Less

本公司与神奈川科学技术研究院（KAST）的研究项目合作，开发了生化分析一体化所必需的基本单元操作。一体化化学系统具有减少试剂消耗、浪费和分析时间等理想特性。利用这些优势，我们已经展示了许多应用，包括溶剂萃取，流动注射分析，激光反应控制，酶促反应和免疫分析。我们还开发了超灵敏的检测装置热透镜显微镜，并将其应用于单细胞成像，微通道内的微空间具有比界面面积大、扩散距离短的特点，这些特点有助于高效提取和混合，比常规方法所需的时间要短得多。湿法分析钴和铁等重金属离子的集成大大缩短了分析时间。的tim ...更多信息 在集成系统中的提取时间比使用分液漏斗和机械振荡器的常规系统短约十倍。此外，我们还集成了使用固体表面的免疫分析系统。这种整合将整个CEA分析所需的时间从2d减少到35 min。用该系统检测血清样品时，与常规ELISA法有很高的相关性。结果表明，热透镜显微镜可以在不使用标记材料的情况下，以极高的灵敏度检测非荧光生物物质，并具有高达100 μ m的空间分辨率。该系统被应用于监测细胞色素c的分布在神经母细胞瘤-胶质瘤杂交细胞培养在玻璃微芯片中制造的微烧瓶。利用该系统成功地监测了细胞凋亡过程中细胞色素c从线粒体向胞浆的释放，为生物化学研究带来了新的进展。少

项目成果

Akihide Hibara: "Integreted Multilayer Flow System on a Microchip"Analytical Sciences. 17. 89-93 (2001)

Akihide Hibara：“微芯片上的集成多层流系统”分析科学。

佐藤記一: "Integration of an Immunosorbent Assay System: Analysis of Secretory Human Immunoglobulin A on Polystyrene Beads in a Microchip"Analytical Chemistry. 72. 1144-1147 (2000)

Kiichi Sato：“免疫吸附测定系统的集成：微芯片中聚苯乙烯珠上的分泌性人免疫球蛋白 A 的分析”分析化学 72. 1144-1147 (2000)

Y.Tanaka et al.: "Non-Contact Photothermal Control of Enzyme Reaction on a Microchip by Using a Compact Diode Laser"J.Chromatogr.A. 894. 45-51 (2000)

Y.Tanaka 等人：“使用紧凑型二极管激光器对微芯片上的酶反应进行非接触式光热控制”J.Chromatogr.A.

田中有希: "Non-Contact Photothermal Control of Enzyme Reaction on a Microchip by Using a Compact Diode Laser"Journal of Chromatography A. 894. 45-51 (2000)

Yuki Tanaka：“使用紧凑型二极管激光器对微芯片上酶反应进行非接触式光热控制”《色谱杂志》A. 894. 45-51 (2000)

Manabu Tokeshi et al.: "Integration of a Microextraction System on a Glass Chip : Ion-Pair Solvent Extraction on Fe(II) with 4,7-Diphenyl-1, 10-phenanthrolinedisulfonic Acid and Trin-octylmethylammonium Chloride"Analytical Chemistry. 72. 1711-1714 (2000)

Manabu Tokeshi 等人：“玻璃芯片上微萃取系统的集成：使用 4,7-二苯基-1, 10-菲咯啉二磺酸和三辛基甲基氯化铵对 Fe(II) 进行离子对溶剂萃取”分析化学。