Construction of Micro Bioanalytical Chemistry Lab,Systems and Single Cellular Biochemistry Integrated on a Glass Chip

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We have developed fundamental unit operations essential to integration of biochemical analysis, collaborating with the research project from Kanagawa Academy of Science and Technology (KAST).The integrated chemical systems have desirable characteristics, such as reduction in reagent consumption, waste and analysis time. Taking these advantages, we have demonstrated many applications, including solvent extraction, flow-injection analysis, laser reaction control, enzymatic reaction and immunoassay. We also developed ultra sensitive detection device, thermal lens microscope and apply this to single-cell imaging.The microspace in the microchannel is characterized by the large specific interfacial area and short diffusion distance, and these characteristics may contribute to highly efficient extraction and mixing with much shorter time than general method. The integration of wet analysis of heavy metal ions such as cobalt and iron leads to a considerable reduction in analyzing time. The tim … More e for extraction in the integrated system was about tenfold shorter than a conventional system using a separately funnel and mechanical shaker. Moreover, troublesome operations could be omitted.We have also integrated the immunoassay system using solid surface. This integration reduced the time necessary for the overall CEA analysis from 2d to 35min. Moreover, when serum samples were assayed with this system, there was a high correlation with the conventional ELISA. These results showed the applicability of integrated analytical systems.Thermal lens microscope could detect nonfluorescent biological substances with extremely high sensitivity without and labeling materials and had a high spatial resolution of 〜1_m. This system was applied to monitoring of cytochrome c distribution in a neuroblastoma-glioma hybrid cell cultured in the microflask fabricated in a glass microchip. Cytochrome c release from mitochondria to cytosol during the apoptosis process was successfully monitored with this system.The results of this research will bring very welcome developments to biochemical studies. Less