Systematic survery for biologically active peptides in the atrium and pheochromocytoma tissue.

1993年我们发现了肾上腺髓质素(AM)，并发现了另一种由肾上腺髓质素前体产生的降压肽-肾上腺髓质素N-末端20肽(PAMP)。我们已经阐明AM是一种新的心血管激素，用于调节循环和体液平衡。此外，AM在体内外对心脏和血管具有保护作用，提示AM可能是一种治疗心血管和炎症性疾病的新药。因此，发现这种新的激素并研究其功能是很重要的。我们同时推进了另一个在猪心房和人嗜铬细胞瘤组织中发现未知血管活性多肽的项目。AM被发现在监测大鼠血小板中升高cAMP的活性。我们利用嗜铬细胞瘤培养细胞中cAMP的升高来寻找生物活性多肽。利用这一方法，我们从嗜铬细胞瘤组织中分离出了几种多肽。这些多肽均为血管活性肠肽、pac…更多的AP、CGRP和PHM。这些多肽是已知的多肽，但我们分离到了存在于嗜铬细胞瘤中的新多肽。许多生物活性多肽都具有C-端酰胺结构，这是生物活性所必需的。我们建立了一种检测具有C-端酰胺结构的多肽的新方法。用这种方法，我们在心房中分离到一种新的14个氨基酸的多肽，其C-末端被酰胺化。这种多肽被命名为氨基霉素。氨基霉素在猪的组织中分布广泛，尤其在脑下垂体、心室和脾中含量丰富。我们建立了人氨基霉素的放射免疫分析方法，并检测了氨基霉素在人体组织中的分布。我们发现人嗜铬细胞瘤组织中含有丰富的氨基霉素。我们从嗜铬细胞瘤中提纯了人氨基霉素，并对其结构进行了测定。此外，还发现氨基霉素在人体血液中以相当高的浓度循环。Amidicin可能是一种新的参与循环调控的多肽。较少

We discovered adrenomedullin (AM) in 1993 and found another hypetensive peptide proadrenomedullin N-terminal 20 peptide (PAMP) which is produced from adrenomedullin precursor. We have clarified that AM is a new cardiovascular hormone to regulate circulation and body fluid homeostasis. Further, AM shows protective actions for the heart and blood vessels in vitro and in vivo, suggesting that AM may be a new drugs for cardiovascular and inflammatory diseases. Thus, it is important to discover the new hormone and to study its function. We have concurrently advanced the another project to discover still unidentified vasoactive peptide in the porcine atrium and human pheochromocytoma tissue.AM was discovered monitoring the activity that elevate cAMP in the rat platelet. We have searched biological active peptide using the cAMP elevation in cultured cells from pheochromocytoma. Using this assay, we isolated several peptides from pheochromocytoma tissue. These peptide were found to be VIP, PAC … More AP, CGRP and PHM. These peptides were known peptides, but we have isolated novel peptide which exist in the the pheochromocytoma. Many biologically active peptides has C-terminal amide structure which is essential for biological activity. We have established a novel method to detect a peptide with C-terminal amide structure. Using this method, we have isolated a novel 14 amino acids peptide in the atrium whose C-terminus was amidated. This peptide was termed amidicin. Amidicin is widely distributed in porcine tissue and is especially abundant in pituitary gland, cardiac ventricle and spleen. We have developed radioimmunoassay for human amidicin, and examined the distribution of amidicin in human tissue. We found that human amidicin is abundant in human pheochromocytoma tissue. We purified human amidicin from pheochromocytoma and determined its structure. Furthermore, amidicin was found to circulate in human blood in a considerable concentration. Amidicin may be a possible new peptide participating in circulation control. Less