Development of tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes

使用温度响应培养皿开发用于角膜疾病的组织工程细胞片移植

• 批准号: 15390530
• 负责人: NISHIDA Koji
• 金额: $ 10.69万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390530/
• 关键词: corneal epithelium; corneal endothelium; regenerative medicine; temperature-responsive culture dish; SP cell; gene transfer; tissue engineering; stem cell; 移植; 培養; 口腔粘膜; 分化転換

We investigated a tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes. In this project, we obtained the following results. Regarding the project for development of corneal epithelial cell sheet transplantation, (1) we have developed a technique to culture corneal epithelial stem cells on temperature-responsive culture dishes, fabricate multilayered corneal epithelial cell sheets and harvest them, (2) we have purified SP cells from limbal epithelium and enriched corneal epithelial progenitor cells by cell sorting using cell surface markers, (3) we have developed surgical techniques for transplantation of the cell sheets, (4) we have developed an effective method for gene transfer to the corneal epithelial cell sheets. Regarding the project for development of corneal endothelial cell sheet transplantation, (5) we have developed culture methods of human corneal endothelial cells, (6) we have developed a technique to culture corneal endothelial cells on temperature-responsive culture dishes, fabricate corneal endothelial cell sheets and harvest them. These results allow us to use our developed technologies for clinical use.

我们研究了使用温度响应培养皿的组织工程细胞片移植治疗角膜疾病。在这个项目中，我们获得了以下结果。关于角膜上皮细胞片移植的开发项目，（1）我们开发了在温度响应培养皿上培养角膜上皮干细胞的技术，制造多层角膜上皮细胞片并收获它们，（2）我们从角膜缘上皮纯化了SP细胞，并通过使用细胞表面标记的细胞分选富集了角膜上皮祖细胞，（3）我们已经开发了细胞片移植的外科技术。（4）我们已经开发了一种有效的将基因转移到角膜上皮细胞片的方法。关于角膜内皮细胞片移植的开发项目，（5）开发了人角膜内皮细胞的培养方法，（6）开发了在温度响应培养皿上培养角膜内皮细胞、制作角膜内皮细胞片并收获它们的技术。这些结果使我们能够将我们开发的技术用于临床。

项目成果

Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface

• DOI: 10.1167/iovs.04-0813
• 发表时间: 2005-05-01
• 期刊: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
• 影响因子: 4.4
• 作者: Hayashida, Y;Nishida, K;Tano, Y
• 通讯作者: Tano, Y

Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo

使用离体制造的自体兔口腔粘膜上皮片重建眼表

• 发表时间: 2004
• 期刊: Investigative Ophthalmology & Vis Sci (in press)
• 作者: Hayashida Y;Nishida;K et al.
• 通讯作者: K et al.

Corneal Reconstruction using tissue-engineered cell sheets comprising autologous oral mucosal epithelium.

使用包含自体口腔粘膜上皮的组织工程细胞片进行角膜重建。

• 发表时间: 2004
• 期刊: New England J. Medicine 351
• 作者: Fujisaki H.;Ebihara T.;Irie S.;Kobayashi K.;Adachi E.;Mochitate K.;Hattori S.;Kazuya Hirai;Goto Mizuki;Goto M;安達 栄治郎;Amano S.;Andrzej Steplewski;Nishida Kohji
• 通讯作者: Nishida Kohji

Rat limbal epithelial side population cells exhibit a distinct expression of stem cell markers that are lacking in side population cells from the central cornea

• DOI: 10.1016/j.febslet.2005.10.047
• 发表时间: 2005-12
• 期刊: FEBS Letters
• 影响因子: 3.5
• 作者: Terumasa Umemoto;M. Yamato;K. Nishida;C. Kohno;Joseph Yang;Y. Tano;T. Okano

Nishida K: "Tissue engineering of the cornea"Cornea. 22. S28-S34 (2003)

西田K：“角膜的组织工程”角膜。