Development of tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes

使用温度响应培养皿开发用于角膜疾病的组织工程细胞片移植

• 批准号: 15390530
• 负责人: NISHIDA Koji
• 金额: $ 10.69万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390530/
• 关键词: corneal epithelium; corneal endothelium; regenerative medicine; temperature-responsive culture dish; SP cell; gene transfer; tissue engineering; stem cell; 移植; 培養; 口腔粘膜; 分化転換

We investigated a tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes. In this project, we obtained the following results. Regarding the project for development of corneal epithelial cell sheet transplantation, (1) we have developed a technique to culture corneal epithelial stem cells on temperature-responsive culture dishes, fabricate multilayered corneal epithelial cell sheets and harvest them, (2) we have purified SP cells from limbal epithelium and enriched corneal epithelial progenitor cells by cell sorting using cell surface markers, (3) we have developed surgical techniques for transplantation of the cell sheets, (4) we have developed an effective method for gene transfer to the corneal epithelial cell sheets. Regarding the project for development of corneal endothelial cell sheet transplantation, (5) we have developed culture methods of human corneal endothelial cells, (6) we have developed a technique to culture corneal endothelial cells on temperature-responsive culture dishes, fabricate corneal endothelial cell sheets and harvest them. These results allow us to use our developed technologies for clinical use.

我们研究了使用温度敏感培养皿的组织工程研究细胞片移植，以进行角膜疾病。 In this project, we obtained the following results.关于角膜上皮细胞片移植的开发项目，（1）我们已经开发了一种技术，用于在温度响应性培养皿上培养角膜上皮干细胞，制造多层角膜上皮细胞表并收集它们，并收集它们，（2）我们使用limbal上皮和富含细胞的细胞（3）细胞（2），（2） （4）我们已经开发了一种将基因转移到角膜上皮细胞表中的有效方法。关于角膜内皮细胞片移植的开发项目，（5）我们已经开发了人角膜内皮细胞的培养方法，（6）我们开发了一种在温度响应性培养菜肴上培养角膜内皮细胞的技术，制造角膜内皮细胞床单并收集它们。这些结果使我们可以将开发的技术用于临床使用。

项目成果

Nishida K: "Tissue engineering of the cornea"Cornea. 22. S28-S34 (2003)

西田K：“角膜的组织工程”角膜。

Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo

使用离体制造的自体兔口腔粘膜上皮片重建眼表

• 发表时间: 2004
• 期刊: Investigative Ophthalmology & Vis Sci (in press)
• 作者: Hayashida Y;Nishida;K et al.
• 通讯作者: K et al.

Corneal Reconstruction using tissue-engineered cell sheets comprising autologous oral mucosal epithelium.

使用包含自体口腔粘膜上皮的组织工程细胞片进行角膜重建。

• 发表时间: 2004
• 期刊: New England J. Medicine 351
• 作者: Fujisaki H.;Ebihara T.;Irie S.;Kobayashi K.;Adachi E.;Mochitate K.;Hattori S.;Kazuya Hirai;Goto Mizuki;Goto M;安達 栄治郎;Amano S.;Andrzej Steplewski;Nishida Kohji
• 通讯作者: Nishida Kohji

Human limbal epithelium contains side population cells expressing the ATP-binding cassette transporter ABCG2

• DOI: 10.1016/j.febslet.2004.03.064
• 发表时间: 2004-05-01
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Watanabe, K;Nishida, K;Tano, Y
• 通讯作者: Tano, Y

Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface.

来自角膜干细胞的功能性生物工程角膜上皮片移植物在温度响应性细胞培养表面上离体扩增。

• 发表时间: 2004
• 期刊: Transplantation 77
• 作者: Nishida K;et al.
• 通讯作者: et al.