Molecular epidemiological study on blood-borne protozoan parasites of small ruminants in China

我国小反刍动物血源性原虫寄生虫的分子流行病学研究

• 批准号: 15405036
• 负责人: SUGIMOTO Chihiro
• 金额: $ 7.49万
• 依托单位: HOKKAIDO UNIVERSITY (2005)Obihiro University of Agriculture and Veterinary Medicine (2003-2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15405036/
• 关键词: Theileria; Babesia; Trypanosoma; China; protozaoa; small ruminant; 住血微生物

Hemoprotozaon parasite closely related to Chinese Theileria species in Sheep was detected from ticks, Haemaphysalis japonicum infesting on Japanese serow and blood samples of serow by using PCR targeting small subunit ribosomal RNA gene of Chinese Theileria sp. from sheep. However, its infectivity to splenectomized sheep was not confirmed when blood from infected serow was injected. Therefore, transmission of Theileria parasites between domestic and wild ruminants was considered no to occur under natural condition.Several methods based on loop-mediated DNA amplification (LAMP) have been developed for diagnoses of diseases caused by hemoprotozoan parasites, including equine Babesia/Trypanosoma, ovine Babesia/Theileria. Specificity and sensitivity of these tests had been demonstrated to be high as compared with the results obtained by PCR tests. Infection rate of Trypanosoma evansi among horses in Xinjian area exceeded 90 % as determined by LAMP. These LAMP methods will be useful for molecular epidemiological studies of hemoprotozoan parasites in China.

采用PCR方法，从羊血蜱、日本血蜱和血清中检测出与羊血蜱亲缘关系较近的血原虫。然而，当注射受感染血清的血液时，未证实其对切除脾的绵羊的感染性。因此，认为在自然条件下不存在家野生反刍动物之间的寄生虫传播。基于环介导的DNA扩增（LAMP）方法已被开发用于诊断由血液原虫寄生虫引起的疾病，包括马巴贝斯虫/锥虫病、羊巴贝斯虫/泰氏杆菌病。与PCR检测结果相比，这些检测的特异性和敏感性均较高。用LAMP法测定新疆地区马中伊氏锥虫的感染率超过90%。这些LAMP方法将为中国血原虫寄生虫的分子流行病学研究提供参考。

项目成果

Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli

利用大肠杆菌表达的截短 NcSRS2 进行酶联免疫吸附试验检测牛体内的犬新孢子虫抗体

• 发表时间: 2005
• 期刊: J Parasitol 91・1
• 作者: Gaturaga;I.;Chahan;B.;Xuan;X.;Huang;X.;Liao;M.;Fukumoto;S.;Hirata;H.;Nishikawa;Y.;Takashima;Y.;Suzuki;H.;Fujisaki;K.;Sugimoto;C.
• 通讯作者: C.

Cloning and expression of mitochondrial heat shock protein 70 of Trypanosoma congolense and potential use as a diagnostic antigen

• DOI: 10.1128/cdli.10.5.926-933.2003
• 发表时间: 2003-09-01
• 期刊: CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
• 作者: Bannai, H;Sakurai, T;Igarashi, I
• 通讯作者: Igarashi, I

Identification of a piroplasm protein of Theileria orientalis that binds to bovine erythrocyte band 3

• DOI: 10.1016/j.molbiopara.2004.05.012
• 发表时间: 2004-10-01
• 期刊: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
• 影响因子: 1.5
• 作者: Kim, JY;Yokoyama, N;Sugimoto, C
• 通讯作者: Sugimoto, C

Molecular characterization of Theileria orientalis piroplasm protein encoded by an open reading frame (To ORF2) in a genomic fragment.

基因组片段中开放阅读框 (To ORF2) 编码的东方泰勒虫梨质蛋白的分子特征。

• 发表时间: 2004
• 期刊: J Vet Med Sci. 66・8
• 作者: Kim JY;Yokoyarna N;Kumar S;Inoue N;Fujisaki K;Sugimoto C
• 通讯作者: Sugimoto C

Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality

• DOI: 10.1016/s0166-6851(03)00199-3
• 发表时间: 2003-10-01
• 期刊: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
• 影响因子: 1.5
• 作者: Fukumoto, S;Xuan, X;Suzuki, H
• 通讯作者: Suzuki, H