GENOME ANALYSIS OF THEILERIA PARASITES

泰勒虫寄生虫的基因组分析

• 批准号: 09044199
• 负责人: SUGIMOTO Chihiro
• 金额: $ 9.34万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044199/
• 关键词: GENOME ANALYSIS; THEILERIA; BAC LIBRARY; PROTOZOA; EXPRESSED SEQUENCE TAG

This project was carried out to analyze genomes of Theileria parasites Eight hundred cDNA clones of Theileria parva was partially sequences to use as expressed sequence tags (ESTs). Some of them were mapped on chromosomes of T.parva. A bacterial artificial chromosome (BAC) library, each of which clones contained 50-200 kilobase pairs of T.parva chromosome fragments, was constructed.The library consisted of 3600 clones that theoretically covered entire T.parva genome. BAC clones that contained gene(s) essential for parasite growth including sporozoite surface protein were identified, which will be useful to analyze genome structures flanking these genes.4 chromosomes of T.sergenti were identified. Size of each chromosome and total genome size of this species was almost same as those of T.parva. Comparative mapping analysis of several homologous genes on T.parva and T.sergenti chromosomes revealed that syntenies among some of the genes are not conserved, which indicated difference of genome structures between these two Theileria species.Although further studies are required to determine whole chromosome structure of T.parva, the results of this project provide a basal information about genome structures of Theileria parasite by establishing BAC library, obtaining ESTs, and comparing two theilerial genomes.

该项目用于分析寄生虫的基因组，八百个theileria parva的cDNA克隆是部分用作表达序列标签（ESTS）的部分序列。其中一些被映射在T.Parva的染色体上。构建了细菌性人造染色体（BAC）文库，每个克隆都构建了50-200千生的T. parva染色体片段。该文库由3600个克隆组成，理论上覆盖了整个T.Parva基因组。鉴定了含有寄生虫生长所必需的基因的BAC克隆，包括孢子岩表面蛋白，这对于分析这些基因两侧的基因组结构很有用。4鉴定了T.sergenti的染色体。该物种的每个染色体和总基因组大小的大小几乎与T.Parva相同。对T. parva和T. serergenti染色体上几个同源基因的比较映射分析表明，某些基因之间的同步并不保守，这表明这两个theileria属物质之间的基因组结构差异。 EST，并比较了两个Teelerial基因组。

项目成果

Iwasaki, T.et al.: "Differentiation and quantification of Theileria sergenti piroplasm types using type-specific monoclonal antibodies" Journal of Veterinary Medical Science. 60. 665-669 (1998)

Iwasaki, T.et al.：“使用类型特异性单克隆抗体对瑟氏泰勒虫梨原体类型进行区分和定量”《兽医医学科学杂志》。

Sako, Y,et al.: "Molecular cloning and characterization of 23 kDa piroplasm surface proteins of Theileria sergenti and T.buffeli" International Journal for Parasitoloy. (In Press). (1999)

Sako, Y 等人：“Theileria sergenti 和 T.buffeli 23 kDa 梨质表面蛋白的分子克隆和表征”国际寄生虫杂志。

Sako, Y, et al.: "Molecular cloning and characterization of 23 kDa piroplasm surface proteins of Theileria sergenti and T.buffeli" Journal of Protozoology Reaserch. in press. (1999)

Sako, Y 等人：“Theileria sergenti 和 T.buffeli 23 kDa 梨质表面蛋白的分子克隆和表征”原生动物学研究杂志。

Sako, Y., et al.: "Molecular cloning of a cysteine protease gene of Theileria sergenti." Journal of Veterinary Medical Science. in press. (1999)

Sako, Y. 等人：“谢氏泰勒虫半胱氨酸蛋白酶基因的分子克隆”。

Iwasaki, T.: "Differentiation and quantification of Theileria sergenti piroplasm types using type-specific monoclonal antibodies." Journal of Veterinary Medical Science. 60. 665-669 (1998)

Iwasaki, T.：“使用类型特异性单克隆抗体对 Sergenti 泰勒虫梨浆体类型进行区分和定量。”