GENOME ANALYSIS OF THEILERIA PARASITES

泰勒虫寄生虫的基因组分析

• 批准号: 09044199
• 负责人: SUGIMOTO Chihiro
• 金额: $ 9.34万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044199/
• 关键词: GENOME ANALYSIS; THEILERIA; BAC LIBRARY; PROTOZOA; EXPRESSED SEQUENCE TAG

This project was carried out to analyze genomes of Theileria parasites Eight hundred cDNA clones of Theileria parva was partially sequences to use as expressed sequence tags (ESTs). Some of them were mapped on chromosomes of T.parva. A bacterial artificial chromosome (BAC) library, each of which clones contained 50-200 kilobase pairs of T.parva chromosome fragments, was constructed.The library consisted of 3600 clones that theoretically covered entire T.parva genome. BAC clones that contained gene(s) essential for parasite growth including sporozoite surface protein were identified, which will be useful to analyze genome structures flanking these genes.4 chromosomes of T.sergenti were identified. Size of each chromosome and total genome size of this species was almost same as those of T.parva. Comparative mapping analysis of several homologous genes on T.parva and T.sergenti chromosomes revealed that syntenies among some of the genes are not conserved, which indicated difference of genome structures between these two Theileria species.Although further studies are required to determine whole chromosome structure of T.parva, the results of this project provide a basal information about genome structures of Theileria parasite by establishing BAC library, obtaining ESTs, and comparing two theilerial genomes.

本项目是为了分析泰勒虫寄生虫的基因组而进行的。对800个小泰勒虫的cDNA克隆进行了部分测序，以用作表达序列标签（EST）。其中一些被映射到染色体上的小锥虫。构建了一个细菌人工染色体（BAC）文库，每个克隆含有50-200对小孢子虫染色体片段，文库包含3600个克隆，理论上覆盖了整个小孢子虫基因组。鉴定了包含寄生虫生长必需基因（包括子孢子表面蛋白）的BAC克隆，这将有助于分析这些基因侧翼的基因组结构。该物种的每条染色体的大小和总的基因组大小几乎相同的T. parva。通过对小泰勒虫和瑟氏泰勒虫染色体上同源基因的比较定位分析，发现部分同源基因间的同线性不保守，表明这两种泰勒虫的基因组结构存在差异，虽然小泰勒虫的染色体结构还有待进一步研究，但本项目的结果通过BAC文库的建立，为了解泰勒虫基因组结构提供了基础信息，获得EST，并比较两个theilirs基因组。

项目成果

Sako, Y,et al.: "Molecular cloning and characterization of 23 kDa piroplasm surface proteins of Theileria sergenti and T.buffeli" International Journal for Parasitoloy. (In Press). (1999)

Sako, Y 等人：“Theileria sergenti 和 T.buffeli 23 kDa 梨质表面蛋白的分子克隆和表征”国际寄生虫杂志。

Iwasaki, T.et al.: "Differentiation and quantification of Theileria sergenti piroplasm types using type-specific monoclonal antibodies" Journal of Veterinary Medical Science. 60. 665-669 (1998)

Iwasaki, T.et al.：“使用类型特异性单克隆抗体对瑟氏泰勒虫梨原体类型进行区分和定量”《兽医医学科学杂志》。

Sako, Y, et al.: "Molecular cloning and characterization of 23 kDa piroplasm surface proteins of Theileria sergenti and T.buffeli" Journal of Protozoology Reaserch. in press. (1999)

Sako, Y 等人：“Theileria sergenti 和 T.buffeli 23 kDa 梨质表面蛋白的分子克隆和表征”原生动物学研究杂志。

Sako, Y., et al.: "Molecular cloning of a cysteine protease gene of Theileria sergenti." Journal of Veterinary Medical Science. in press. (1999)

Sako, Y. 等人：“谢氏泰勒虫半胱氨酸蛋白酶基因的分子克隆”。

Sako, Y, et al.: "Expression of a major piroplasm surface of Theileria sergenti in sporozoite stage." Journal of Veterinary Medical Science. in press. (1999)

Sako, Y 等人：“谢氏泰勒虫在子孢子阶段的主要梨质表面的表达。”