Effects of oral treatment with plant-expressed cytokines for mucosal barrier formation and maturation

植物表达细胞因子口服治疗对粘膜屏障形成和成熟的影响

• 批准号: 15380208
• 负责人: SUGIMOTO Chihiro
• 金额: $ 10.05万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380208/
• 关键词: Transgenic plants; Plant biothecnology; Mucosal immunity; Oral immunity; 形質転換植物; サイトカイン

The aim of this project is to determine effects of oral treatment with plant-expressed cytokines for mucosal barrier formation and maturation. We also examined protective activity of oral cytokine treatment against pathogens. In a mouse infection model with Listeria monocytogenes, oral treatment with human interferon alfa reduced bacterial burden in spleen and liver. Plant-expressed recombinant protein exhibited similar effect. We obtained information of genes activated with oral treatment with human interferon alfa, and designed primers for the detection of corresponding genes in cattle. Activation of these genes will be used for evaluation of oral interferon treatment in domestic animals. Effect of treatment with transforming growth factor beta was evalualted in mouse infection model with protozoan parasites including Toxoplasma gondii, Babesia microti, Babesia rhodhaini, Plasmodium berghei, Trypanosoma congolense. The treatment reduced parasitemia of T.congolense, which indicates treatment with transforming growth factor beta may activate host defense activity against pathogens.

本项目的目的是确定用植物表达的细胞因子进行口腔治疗对粘膜屏障形成和成熟的影响。我们还检查了口服细胞因子治疗对病原体的保护活性。在单核细胞增生李斯特菌感染的小鼠模型中，口服人干扰素α可降低脾脏和肝脏中的细菌负荷。植物表达的重组蛋白具有类似的效果。我们获得了口服人干扰素α激活的基因信息，并设计了用于检测牛中相应基因的引物。这些基因的激活将用于评价家畜口服干扰素治疗。在小鼠感染弓形虫、田鼠巴贝虫、罗海巴贝虫、伯氏疟原虫、刚果锥虫等原虫模型中评价了转化生长因子β的治疗效果。该处理减少了刚果锥虫的寄生虫血症，这表明用转化生长因子β处理可以激活宿主对病原体的防御活性。

项目成果

Mammalian cytokines produced in plants

植物中产生的哺乳动物细胞因子

• 发表时间: 2004
• 期刊: Encyclopedia of Plant & Crop Science (Oona Schmid, Editor)(Marcel Dekker, Inc.)
• 作者: Sugimoto;C.
• 通讯作者: C.

Increase of antibody fiter against Leucocytozoon caulleryi by oral administration of recombinant R7 antigen

口服重组R7抗原提高抗白细胞虫的抗体适​​配度

• 发表时间: 2005
• 期刊: J Vet Med Sci. 67-2
• 作者: T.Kawasaki;F.Nagatsugi;Md.M.Ali;M.Maeda;K.Sugiyama;K.Hori;S.Sasaki;Ito A.
• 通讯作者: Ito A.

Encyclopedia of Plant & Crop Science (Oona Schmid, Editor)

植物百科全书

• 发表时间: 2004
• 作者: Wang;D.W.;Ueda;S.;Uyeda;I.;Yagihashi;H.;Sekiguchi;H.;Tacahashi;Y.;Sato;M.;Ohya;K.;Sugimoto;C.;Matsumura;T.;Chihiro Sugimoto
• 通讯作者: Chihiro Sugimoto

Increase of antibody titer against Leucocytozoon caulleryi by oral administration of recombinant R7 antigen

• DOI: 10.1292/jvms.67.211
• 发表时间: 2005-02-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Ito, A;Gotanda, T;Matsumura, T
• 通讯作者: Matsumura, T

Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector

基因插入位置对三叶草黄脉病毒表达载体遗传稳定性的影响

• 发表时间: 2003
• 期刊: J.Gen.Plant Pathol. 69
• 作者: Wang;D.W.
• 通讯作者: D.W.