Non-contact, highly-functional skin diagnosis based on nonlinear optical effect in biological tissue

我们提出了一种基于生物组织中非线性光学效应的光学探针方法，可以用来表征人体真皮中胶原纤维的取向。胶原蛋白的特定探测能力来自于使用由组织中的胶原蛋白分子诱导的二次谐波产生（SHG）光。我们首先进行了基于反射型偏振测量的SHG光的人体组织中的胶原纤维的取向的测量。所得的SHG雷达图显示胶原纤维的绝对取向方向和组织化程度。我们将所提出的方法应用于几种人体组织，并观察到胶原蛋白取向的显着差异，这与组织学结果是一致的。接下来，使用二维和三维SHG成像方法，真皮组织中胶原纤维的详细分布被可视化为高对比度、高分辨率的SHG图像。此外，联合使用SH ...更多信息 G成像和偏振测量提供真皮中胶原纤维取向的分布，这在维持皮肤的机械性质中起重要作用。作为皮肤诊断的应用实例，我们展示了利用真皮胶原的SHG成像监测皮肤烧伤和皮肤老化。在皮肤烧伤中，通过深度分辨SHG成像和SHG交叉扫描成像，可以清楚地显示真皮中的烧伤区域和深度。在皮肤老化方面，我们应用SHG显微镜测量了年轻小鼠、自然老化小鼠和光老化小鼠背部真皮的胶原纤维分布。结果表明，光老化小鼠表皮-真皮交界处的SHG信号明显减弱，而自然老化小鼠与年轻小鼠之间的SHG信号无明显差异。该方法将是一个强有力的工具，在体内测量真皮胶原纤维，可用于监测皮肤疾病和化妆品。少

We have proposed an optical probe method based on nonlinear optical effect in biological tissue that can be used to characterize the orientation of collagen fibers in human dermis. A specific probing ability for collagen results from the use of second-harmonic-generation (SHG) light induced by collagen molecules in the tissue. We first carried out the measurement of collagen fiber orientation in human tissues based on reflection-type polarization measurement of SHG light. The resulting SHG radar graph shows the direction of the absolute orientation and the organization degree of collagen fibers. We applied the proposed method to several human tissues and observed significant differences in collagen orientation, which are consistent with histological findings. Next, using two-dimensional and three-dimensional SHG imaging methods, the detailed distributions of collagen fiber in dermis tissues are visualized as highly-contrast, highly-resolution SHG images. Furthermore, combined use of SH … More G imaging and polarization measurements provides distribution of collagen fiber orientation in the dermis, which plays an important role in maintaining the mechanical property of the skin. As application examples of skin diagnosis, we demonstrated monitoring of skin burn and skin aging with SHG imaging of dermal collagen. In skin burn, burned area and depth in the dermis are clearly visualized by depth-resolved SHG imaging and SHG cross-seotional imaging. In skin aging, we applied SHG microscopy to measure collagen fiber distribution of back dermis in a young mouse, a natural-aged one, and a photo-aged one. The resulting SHG microphotographs indicated that the SHG signal is significantly decreased around an epidermis dermis boundary in the photo-aged mouse whereas there are no remarkable differences between the naturalaged mouse and the young one. The proposed method will be a powerful tool for in vivo measurement of dermal collagen fiber which can be applied for monitoring of skin diseases and cosmetics. Less