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Assessment of Central Dopamine Release by Exercise in Hunian in vivo.

湖南人体内运动中枢多巴胺释放的评估。

基本信息

批准号：
16300199
负责人：
ITOH Masatoshi
金额：
$ 6.14万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Assessment of central dopamine (DA) release in vivo in human using D_2 PET mapping is a novel technique for neuroscience. We applied this technique to assess DA release by enduring ergometer exercise. We hypothesized that failure of the previous studies to reveal DA release by exercise may be from technical problems. They measured D_2 receptor distribution after completion of exercise. By using a modified ergometer that allows bicycling while lying on PET couch, we carried out raclopride PET simultaneously during exercise. To minimize head movements due to bicycling, we used a plastic face mask for each subject developed for conformal radiotherapy. In addition head movements were mathematically corrected using an image alignment algorithm equipped in SPM5 (Functional Imaging Laboratory, London). Eight healthy male volunteers aged 21.4±2.0 (s.d.) years collaborated the study. They made an ergometer exercise of moderate intensity (VO2Max 35〜60%) for 50 minutes. [^<11>C]Raclopride was injected intravenously at 20 minutes after start of the exercise and then a sequential PET images were collected using 3D positron camera (Shimadzu 2400W) with scatter correction. ROIs were placed at the caudate, putamen, and cerebellum of each side. Choosing cerebellum as a reference tissue we calculated raclopride binding potential (BP) to D_2 receptors using the simplified reference tissue model (Lammersma and Gunn), Logan noninvasive model, and Ichise model. The BP in the striatum were significantly reduced at exercise (-12.9〜17.0%, P<0.01) when compared to a control condition (lying on PET couch only). The reductions were almost similar between the caudate and the putemen and between hemispheres. The results strongly support DA release at this ergometer exercise condition. Dopamine release in our brain may explain refreshing feeling after exercise and its habituations.

应用D_2PET定位技术评估人体中枢多巴胺释放是神经科学领域的一项新技术。我们应用这项技术通过耐力测功机运动来评估DA的释放。我们推测，之前的研究未能揭示运动释放DA的原因可能是技术问题。他们在运动结束后测量了D_2受体的分布。通过使用改进的测功器，允许躺在PET沙发上骑自行车，我们在锻炼期间同时进行了拉氯普利PET检查。为了最大限度地减少骑自行车导致的头部移动，我们为每个受试者使用了一个塑料面罩，用于适形放射治疗。此外，使用SPM5(伦敦功能成像实验室)配备的图像对齐算法对头部运动进行了数学校正。8名健康男性志愿者，年龄21.4±2.0(S.D.)多年的合作完成了这项研究。他们进行中等强度(最大摄氧量35~60%)的功率运动50min。在运动开始后20分钟静脉注射雷氯普利，然后使用带散射校正的3D正电子相机(岛津2400W)采集序列PET图像。在两侧尾状核、壳核和小脑放置感兴趣区。以小脑为参比组织，用简化的参比组织模型(Lammersma和Gunn)、Logan无创模型和Ichise模型计算了拉氯必利与D_2受体的结合势(BP)。与对照组(只躺在PET沙发上)相比，运动后纹状体的血压显著降低(-12.9~17.0%，P&lt；0.01)。尾状核和丘脑之间以及大脑半球之间的减少几乎是相似的。这一结果有力地支持了在这种功率运动条件下DA的释放。我们大脑中的多巴胺释放可能解释了运动后的清新感觉及其习惯。