Basic study on the production of somatic nuclear transferred chicken.

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1. Production of nuclear transferred GGC.:Attempts were made to produce nuclear transferred gonadal germ cells (GGCs) using karyoplast produced from fibroblast. Karyoplasts were produced by using centrifuging fibroblast at 10,000 g 1 hour in the presence of 0.1% cytochalasin B. Average diameter of the karyoplasts were 10% smaller tcompared with intact fibroblast. Six nuclear transferred GGCs were produced using karyoplasts by electrofusion.2. Production of intra-Genus nuclear transferred GGCs.Attempts were made to produce intra-genus nuclear transferred GGC using chicken and quail. After electrofusion, 0.5-1.2 % of the nuclear GGCs was produced.3. Effects of soft X exposure on the migrating ability of primordial germ cells.Effects of soft X exposure on the migrating ability of primordial germ cells were examined. Results showed that the PGCs retain their migration ability toward gonadal ridge after transfer even after soft exposure.