Basic study on the production of somatic nuclear transferred chicken.

体细胞核移植鸡生产基础研究。

• 批准号: 16380184
• 负责人: TAJIMA Atsushi
• 金额: $ 9.54万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380184/
• 关键词: Primordial Germ Cells; Nuclear transfer; chicken; ウズラ; 鳥類; 生殖系列; 電気融合; 移住能力

1. Production of nuclear transferred GGC.:Attempts were made to produce nuclear transferred gonadal germ cells (GGCs) using karyoplast produced from fibroblast. Karyoplasts were produced by using centrifuging fibroblast at 10,000 g 1 hour in the presence of 0.1% cytochalasin B. Average diameter of the karyoplasts were 10% smaller tcompared with intact fibroblast. Six nuclear transferred GGCs were produced using karyoplasts by electrofusion.2. Production of intra-Genus nuclear transferred GGCs.Attempts were made to produce intra-genus nuclear transferred GGC using chicken and quail. After electrofusion, 0.5-1.2 % of the nuclear GGCs was produced.3. Effects of soft X exposure on the migrating ability of primordial germ cells.Effects of soft X exposure on the migrating ability of primordial germ cells were examined. Results showed that the PGCs retain their migration ability toward gonadal ridge after transfer even after soft exposure.

1.核转移GGC的生产：尝试使用由成纤维细胞产生的核质体产生核转移的性腺生殖细胞（GGC）。在0.1%细胞松弛素B的存在下，通过使用10，000 g/小时的成纤维细胞产生有核体。核质体的平均直径比完整的成纤维细胞小10%。利用核质体电融合技术获得了6个核转移GGC.属内核转移GGC的生产：尝试使用鸡和鹌鹑生产属内核转移GGC。电融合后，产生0.5- 1.2%的核GGC.软X射线照射对原始生殖细胞迁移能力的影响研究了软X射线照射对原始生殖细胞迁移能力的影响。结果表明，移植后的PGCs即使在软暴露后仍保持着向性腺嵴迁移的能力。

项目成果

Production of somatic nuclear transferred gonadal germ cells (GGCs) using somatic. karyoplast in domestic chicken.

使用体细胞生产体细胞核移植性腺生殖细胞（GGC）。

• 发表时间: 2006
• 期刊: World's Poultry Science Journal, Supplement. 62
• 作者: Satoru Araya;Yusaku Kohara;Yukio Kanai;Atsushi Tajima
• 通讯作者: Atsushi Tajima

Simplified DNA extraction methods for sexing chick embryos

• DOI: 10.2141/jpsa.41.147
• 发表时间: 2004-04-01
• 期刊: Journal of Poultry Science
• 影响因子: 1.5
• 作者: Minematsu, Takeo;Sugiyama, Misato;Kanai, Yukio
• 通讯作者: Kanai, Yukio

Transformation of skeletal muscle from fast to slow-twitch during acquisition of cold tolerance in the chick.

在雏鸡获得耐冷能力的过程中，骨骼肌从快肌纤维转变为慢肌纤维。

• 发表时间: 2005
• 期刊: Endocrionlogy 146
• 作者: M.Hirabayashi;D.Ijiri;Y.Kamei;A.Tajima;Y.Kanai
• 通讯作者: Y.Kanai

Production of inter-genus somatic nuclear transferred GGCs in avian

鸟类属间体细胞核转移 GGC 的产生

• 发表时间: 2006
• 期刊: World's Poultry Science Journal, Supplement. 62
• 作者: Susumu Ishiguro;Yukio Kanai;Atsushi Tajima
• 通讯作者: Atsushi Tajima

Effects of Ultraviolet Irradiation on the Migratory ability of Primordial Germ Cells in the Domestic Chicken

紫外线照射对家鸡原始生殖细胞迁移能力的影响

• 发表时间: 2004
• 期刊: Journal of Poultry Science 41
• 作者: Minematsu T;Kanai Y;Tajima A.
• 通讯作者: Tajima A.