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Molecular mechanism of viral pathogenecity, cytotoxicity and establishment of persistent infection

病毒致病性、细胞毒性和持续感染建立的分子机制

基本信息

批准号：
16390134
负责人：
ITO Yasuhiko
金额：
$ 9.66万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

HeLa cells persistently infected with hPIV-2 Toshiba strain were established. Until 50 passages, multinucleated giant cells were detected all the time. The greater part of HeLa/hPIV2/Toshiba cells are steady states, but some fractions show CPE and eventually die. Thus, hPIV2 persistently infected cells are considered to be a mixture of carrier and steady states. Establishing processes of persistent infection by paramyxoviruses were classified into 5types.A mixture of steady state and carrier state was often found in persistent infection by paramyxoviruses. We devised a quantitative method for analyzing establishing-efficiency of persistent infection. The efficiency of hPIV2 CA and SV5 T1 strains belonging to type I was high, that is, 0.1〜0.3 and the efficiency of SV5 WR belonging to type II was also high, approximately 0.1, though the virus had no ability to immediately establish persistent infection (steady state). The efficiency of SV41 belonging to type IV was about 0.0007 and was almost same as that of hPIV2 Toshiba strain. A mixture of steady state and carrier state is often found in persistent infection by paramyxoviruses. Furthermore, the establishing efficiency of various SeV pi strain was further analyzed in detail. The efficiency of parent recombinant Sendai virus (rSeV (PA) ), rSeV (Ppi) and rSeV (HNpi) was below the limit of detection, while that of rSeV (Lpi) was nearly 1. Unexpectedly, rSeV (Mpi) and rSeV (Fpi) were found to be capable of forming persistently-infected cells, although the efficiency was around 0.001, indicating that both Fpi and Mpi proteins contribute to the establishing efficiency of persistent infection of SeVpi.

建立了持续感染hPIV-2 Toshiba株的HeLa细胞。直到50代，一直检测到多核巨细胞。HeLa/hPIV2/Toshiba细胞大部分处于稳定状态，但部分呈CPE并最终死亡。因此，hPIV2持续感染的细胞被认为是载体和稳定状态的混合物。副粘病毒持续感染的建立过程可分为5种类型。副粘病毒持续感染常出现稳态和载体状态的混合。我们设计了一种定量分析持续感染建立效率的方法。属于I型的hPIV2 CA和SV5 T1株的效率较高，为0.1 ~ 0.3；属于II型的SV5 WR株的效率也很高，约为0.1，但病毒没有立即建立持续感染（稳态）的能力。属于IV型的SV41的效率约为0.0007，与hPIV2东芝菌株的效率基本相同。副粘病毒持续感染常出现稳态和载体状态的混合。进一步详细分析了各种SeV pi菌株的建立效率。重组仙台病毒亲本（rSeV (PA)）、rSeV （Ppi）和rSeV （HNpi）的效率均低于检测限，而rSeV （Lpi）的效率接近1。出乎意料的是，rSeV （Mpi）和rSeV （Fpi）被发现能够形成持续感染的细胞，尽管效率约为0.001，这表明Fpi和Mpi蛋白都有助于建立SeVpi持续感染的效率。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A mutant fusion (F) protein of simian virus 5 induces hemagglutinin-neuraminidase-independent syncytium formation despite the internalization of the F protein

DOI：
10.1016/j.virol.2005.11.014
发表时间：
2006-03-30
期刊：
VIROLOGY
影响因子：
3.7
作者：
Tsurudome, M;Ito, M;Ito, Y
通讯作者：
Ito, Y

Cytological properties of stromal cells derived from giant cell tumor of bone (GCTSC) which can induce osteoclast formation of human blood monocytes without cell to cell contact

DOI：
10.1016/j.orthres.2005.01.004
发表时间：
2005-09-01
期刊：
JOURNAL OF ORTHOPAEDIC RESEARCH
影响因子：
2.8
作者：
Nishimura, M;Yuasa, K;Ito, Y
通讯作者：
Ito, Y

Regulation of lymphocyte activation through CD98 is independent of IL-2/IL-2R system. Biomedical Research

通过 CD98 调节淋巴细胞活化独立于 IL-2/IL-2R 系统。

DOI：
发表时间：
期刊：
Biomedical Research (In press)
影响因子：
0
作者：
M.Nishimura et al.;M.Nishimura et al.;M.Nishio et al.;M.Nishio et al.;Makoto Nishimura;Morihiro Ito;H.Komada et al.
通讯作者：
H.Komada et al.

Regulation of lymphocyte activation through CD98 is independent of IL-2/IL-2R system.

通过 CD98 调节淋巴细胞活化独立于 IL-2/IL-2R 系统。

DOI：
发表时间：
期刊：
Biomedical Research (In press)
影响因子：
0
作者：
M.Nishimura et al.;M.Nishimura et al.;M.Nishio et al.;M.Nishio et al.;Makoto Nishimura;Morihiro Ito;H.Komada et al.;H.Komada et al.;M.Tsurudome et al.;H.Komada et al.
通讯作者：
H.Komada et al.

Human Parainfluenza Virus Type 4 Incapable of Evading the Interferon-Induced Antiviral Effect

人类副流感病毒 4 型无法逃避干扰素诱导的抗病毒作用

DOI：
发表时间：
2005
期刊：
J.Virology 79・23
影响因子：
0
作者：
M.Nishimura et al.;M.Nishimura et al.;M.Nishio et al.;M.Nishio et al.
通讯作者：
M.Nishio et al.

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ITO Yasuhiko其他文献

Analysis Technique of Millimeter-Wave Propagating Modes in an Oversized Corrugated Waveguide Using Developed Beam Profile Monitors

使用开发的光束轮廓监测仪分析超大波纹波导中的毫米波传播模式

DOI：
10.1585/pfr.13.3405036
发表时间：
2018
期刊：
Plasma and Fusion Research
影响因子：
0.8
作者：
SHIMOZUMA Takashi;KOBAYASHI Sakuji;ITO Satoshi;OKADA Kohta;ITO Yasuhiko;YOSHIMURA Yasuo;IGAMI Hiroe;TAKAHASHI Hiromi;TSUJIMURA Toru;MIZUNO Yoshinori;KUBO Shin
通讯作者：
KUBO Shin