Developement of cell adhesion factor like anti-viral agents

细胞粘附因子类抗病毒药物的开发

• 批准号: 09557027
• 负责人: ITO Yasuhiko
• 金额: $ 4.86万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557027/
• 关键词: HIV; PARAMYXOVIRUS; FRP-1; CD98; PROTEIN KINASE; インテグリン

We analyzed the mechanisms of virus (paramyxoviruses and HIV)-induced cell damage. We isolated 9 monoclonal antibodies directed against FRP-l molecules and clarified their abilities. Anti-FRP- 1 antibodies can be classified into three groups, namely, cell fusion enhancing antibodies, cell fusion suppressing antibodies and antibody showing no activity. Anti-FRP- 1 antibody, both cell fusion enhancing and suppressing antibodies, can stimulate intracellular signaling. However, we could not find the difference between these antidodies-induced signaling. We also isolate 19 monoclonal antibodies directed against murine FRP-1. Murine FRP-1 was found to be alloantigens and to be identical to Ly10 alloantigens. We showed that there were two different FRP-1-mediated signal pathways, that is, positive and negative signaling. Furthermore, we cloned FRP-1/CD90 light chain cDNA and carried out chromosomal mapping of FRP-1/CD90 light chain. We found an interesting cell fusion induction system, in which fusion (F) protein of paramyxovirus alone can induce multinucleated giant cells. We also found that there were two kinds of F protein of SV 5, that is, one F protein of SV 5 has an ability for induction cell fusion without HN protein and other F protein can induce cell fusion by an assistance of HN protein. There are three amino acid differences between these F proteins, and we identified amino acid responsible for inducibility of cell fusion by F protein alone.

我们分析了病毒（副粘病毒和HIV）诱导细胞损伤的机制。我们分离了9个针对frp - 1分子的单克隆抗体，并明确了它们的能力。抗frp - 1抗体可分为三大类，即增强细胞融合抗体、抑制细胞融合抗体和无活性抗体。抗frp - 1抗体是一种增强和抑制细胞融合的抗体，可以刺激细胞内信号传导。然而，我们没有发现这些抗毒剂诱导的信号传导之间的差异。我们还分离出19种针对小鼠FRP-1的单克隆抗体。小鼠FRP-1与Ly10同种异体抗原相同。我们发现有两种不同的frp -1介导的信号通路，即正信号通路和负信号通路。此外，我们克隆了FRP-1/CD90轻链cDNA，并进行了FRP-1/CD90轻链的染色体定位。我们发现了一个有趣的细胞融合诱导系统，其中副粘病毒的融合(F)蛋白可以单独诱导多核巨细胞。我们还发现SV - 5的F蛋白有两种，即SV - 5的一种F蛋白在没有HN蛋白的情况下具有诱导细胞融合的能力，而另一种F蛋白在HN蛋白的辅助下诱导细胞融合。这些F蛋白之间存在三个氨基酸差异，我们确定了F蛋白单独诱导细胞融合的氨基酸。

项目成果

Hideki Tsumura: "Detection of endogenous retrovirus antigens in NOD mouse pancreatic beta-cells" Lab.Anim.32. 86-94 (1998)

Hideki Tsumura：“NOD 小鼠胰腺 β 细胞中内源性逆转录病毒抗原的检测”Lab.Anim.32。

K.Okamoto, M.Tsurudome, S.Ohgimoto, M.Kawano, M.Nishio, H.Komada, M.Ito, Y.Sakakura, and Y.Ito: "An Anti-Fusion Regulatory Protein-1 (FRP-1) Monoclonal Antibody Suppresses Human Parainfluenza Type2 Virus-Induced Cell Fusion" J.Gen.Virology. 78. 83-89 (199

K.Okamoto、M.Tsurudome、S.Ohgimoto、M.Kawano、M.Nishio、H.Komada、M.Ito、Y.Sakakura 和 Y.Ito：“一种抗融合调节蛋白-1 (FRP-1)

Masato Tsurudome: "Primary Structure of the Light Chain of Fusion Regulatory Protein-1/CD98/4F2 Predicts a Protein with Multiple Transmembrane Domains That Is Almost Identical to the Amino Acid Transporter E16" J.Immunology. in press.

Masato Tsurudome：“融合调节蛋白-1/CD98/4F2 轻链的一级结构预测具有多个跨膜结构域的蛋白质，该结构域与氨基酸转运蛋白 E16 几乎相同”J.Immunology。

H.Tsumura, M.Miyazawa, S.Pgawa, JZ.Wang, Y.Ito and K.Shimura: "Detection of endogenous retrovirus antigens in NOD mouse pancreatic beta-cells" Lab.Anim.32. 86-94 (1998)

H.Tsumura、M.Miyazawa、S.Pgawa、JZ.Wang、Y.Ito 和 K.Shimura：“NOD 小鼠胰腺 β 细胞中内源性逆转录病毒抗原的检测”Lab.Anim.32。

Hideki Tsumura: "Isolation and Characterization of Monoclonal Antibodies Directed Against Murine FRP-1/CD98/4F2 Heavy Chain:" Immunology and Cell Biology.

Hideki Tsumura：“针对鼠 FRP-1/CD98/4F2 重链的单克隆抗体的分离和表征：”免疫学和细胞生物学。