Developement of cell adhesion factor like anti-viral agents

我们分析了病毒(副粘病毒和HIV)诱导细胞损伤的机制。我们分离了9株抗纤维蛋白-L分子的单抗，并对其功能进行了鉴定。抗FRP-1抗体可分为三类，即细胞融合促进抗体、细胞融合抑制抗体和无活性抗体。抗FRP-1抗体既有促进细胞融合的作用，也有抑制细胞融合的作用，可以刺激细胞内信号传导。然而，我们找不到这些解毒剂诱导的信号之间的差异。我们还分离了19株针对小鼠FRP-1的单抗。小鼠FRP-1为同种异体抗原，与Ly10同种异体抗原相同。我们发现FRP-1有两条不同的信号通路，即正信号通路和负信号通路。此外，我们还克隆了FRP-1/CD90轻链基因，并对FRP-1/CD90轻链进行了染色体定位。我们发现了一个有趣的细胞融合诱导系统，在这个系统中，副粘病毒的融合蛋白(F)单独可以诱导多核巨细胞。我们还发现了SV5的两种F蛋白，即一种不含HN蛋白的SV 5的F蛋白具有诱导细胞融合的能力，另一种F蛋白可以在HN蛋白的辅助下诱导细胞融合。这些F蛋白之间有三个氨基酸差异，我们鉴定了与F蛋白单独诱导细胞融合有关的氨基酸。

We analyzed the mechanisms of virus (paramyxoviruses and HIV)-induced cell damage. We isolated 9 monoclonal antibodies directed against FRP-l molecules and clarified their abilities. Anti-FRP- 1 antibodies can be classified into three groups, namely, cell fusion enhancing antibodies, cell fusion suppressing antibodies and antibody showing no activity. Anti-FRP- 1 antibody, both cell fusion enhancing and suppressing antibodies, can stimulate intracellular signaling. However, we could not find the difference between these antidodies-induced signaling. We also isolate 19 monoclonal antibodies directed against murine FRP-1. Murine FRP-1 was found to be alloantigens and to be identical to Ly10 alloantigens. We showed that there were two different FRP-1-mediated signal pathways, that is, positive and negative signaling. Furthermore, we cloned FRP-1/CD90 light chain cDNA and carried out chromosomal mapping of FRP-1/CD90 light chain. We found an interesting cell fusion induction system, in which fusion (F) protein of paramyxovirus alone can induce multinucleated giant cells. We also found that there were two kinds of F protein of SV 5, that is, one F protein of SV 5 has an ability for induction cell fusion without HN protein and other F protein can induce cell fusion by an assistance of HN protein. There are three amino acid differences between these F proteins, and we identified amino acid responsible for inducibility of cell fusion by F protein alone.