DEVELOPMENT OF NEW MUMPS VIRUS VACCINE BY USING GENE MANIPULATION

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For development of new Mumps virus vaccine, we have tried to establishe the reverse genetice of parainfluenza viruses, especially HPIV-2. We examined two systems, that is, helper virus system and Vaccinia virus-T7 system. After a disperate effort we managed to succeed to some extent. Furthermore, we investigated basic virology on paramyxovirus and clarified the following results ; Interaction of the NP and V proteins of HPIV-2 was investigated. When the NP protein was co-expressed with the V protein, a part of NP proteins was translocated into the nuclei. These findings suggested that the NP proteins interact with the V proteins. We examined interaction of the NP protein and the P,V protein or deletion mutants of V protein by immunofluorescent and co-immunoprecipitation + Western blotting analyzes. Co-expressing with the V protein or the N-terminal fragment (a.a.1 to 46) , the NP protein was detected diffusely in the nuclei of the transfected cells, and was also detected in cytoplasmic inclusions, and the NP protein was coprecipitated with the P,V,and V (1-164) proteins by specific antibody. These findings indicate that the V proteins have an ability to bind the NP proteins. At present we are preparing full-length cDND clones for parainfluenza type 2 virus and HPIV-2-MuV chimera. We think that development of new mumps virus vaccine will be prepared in the future by gene engineering technique established by this study