Translation research for predicting the prognosis of oral malignant tumors.

E1 AF是一种ets-致癌基因家族转录因子，并且已显示其上调多种MMP，而MMP-2（一种有效的细胞外基质降解酶）不上调。氯霉素乙酰转移酶（CAT）测定用于研究E1 AF是否能够上调MT 1-MMP，其已知激活MMP-2。通过与E1 AF表达载体共转染，在MT 1-MMP启动子控制下的CAT报告基因的表达增加约40倍。25例舌鳞癌组织中E1 AF、MT 1-MMP的mRNA表达水平呈协同增加，提示E1 AF正调控MT 1-MMP基因的转录，它通过将pro-MMP-2转化为活性-MMP-2，在舌鳞状细胞癌的侵袭和转移中发挥重要作用。2.探讨Bcl-2家族基因表达与化疗和放疗作用的关系。 ...更多信息 离子疗法Bax被证明可以降低所有癌症病例中的表达。Bcl-2的高表达水平在化疗和放疗耐药的病例中观察到。应用逆转录-聚合酶链反应（RT-PCR）方法检测19例鳞癌和4例癌前病变组织中Ets-1、Ets-2和GnT-V mRNA的表达，并与正常舌组织进行比较。观察GnT-V免疫组化检测结果及其与临床病理特征的关系。所有癌前病变和鳞状细胞癌的情况下，检查显示与正常舌组织相比，Ets-1的mRNA水平显着升高的实时PCR。Ets-2和GnT-V mRNA水平在SCC病例中也有所增加，但与之相反，与正常组织相比，癌前病变中Ets-2和GnT-V mRNA水平并未增强。GnT-V mRNA表达水平与Ets-1、Ets-2表达水平呈正相关（rs=0.7;p<0.01）。Ets-1和GnT-V在SCC中的表达与肿瘤浸润方式和颈淋巴结转移呈正相关。而Ets-2的表达与这些特征关系不大。这些结果表明，Ets-1在调节人舌鳞状细胞癌中的GnT-V中起着重要作用，并且可能通过GnT-V的作用参与恶性肿瘤的可能性。

E1AF is an ets-oncogene family transcription factor and it has been shown to upregulate multiple MMPs whereas MMP-2, a potent extracellular matrix degrading enzyme, is not upregulated. Chloramphenicol acetyltransferase (CAT) assay was utilized to investigate whether E1AF is able to upregulate MT1-MMP, which is known to activate MMP-2. Expression of the CAT reporter gene under the control of the MT1-MMP promoter was increased approximately 40-fold by co-transfection with the E1AF expression vector. Real-time RT-PCR study was carried out in 25 patients with tongue squamous cell carcinoma, and the mRNA expression level of E1AF,MT1-MMP was synergistically increased.These results indicate that E1AF positively regulates transcription from MT1-MMP genes, which plays an important role in invasion and metastasis of squamous cell carcinoma of the tongue by converting pro-MMP-2 into active-MMP-2.We investigated the relationship between Bcl-2 family gene expression and the effect of chemo- and rad … More iotherapy. Bax was shown to reduce the expression in all carcinoma cases. High expression levels of Bcl-2 were observed in resistant cases for chemo- and radiotherapy. These results suggest that examination of Bcl-2 family gene expression levels would be the predictive factors of sensitivity for preoperative therapies.Ets-1,Ets-2, and GnT-V mRNA expression were analyzed by the reverse transcription-polymerase chain reaction method in biopsy materials of 19 SCC cases and 4 premalignant lesions and compared with the expression levels of normal tongue tissues. Immunohistochemical detection of GnT-V and the relationship between clinico-pathological features were investigated. All premalignant lesions and SCC cases examined showed significantly escalated Ets-1 mRNA levels compered with normal tongue tissues by real-time PCR. Ets-2 and GnT-V mRNAs levels were also increased in SCC cases, but in contrast, their levels were not enhanced in premalignant lesions in comparison to normal tissues. The levels of GnT-V mRNA were correlated with Ets-1 and Ets-2 expression (rs=0.7;p<0.01). Ets-1 and GnT-V expression in SCC were positively associated with the tumor invasive pattern and cervical lymph node metastasis. On the contrary, Ets-2 expression has little relationship with these characteristics. These results suggest that Ets-1 plays a significant role in regulating GnT-V in human tongue squamous cell carcinoma and might be involved in the potential for malignancy via the action of GnT-V. Less