DNA MICROARRAY AND EXPRESSION CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 TNPRC的DNA微阵列和表达核心(微阵列核心)目前提供以下服务： A.DNA微阵列： 1)微阵列实验设计： 2)斑点或双色微阵列实验： 3)基因芯片数据分析 4)小样本放大 5)聚合酶链式反应阵列 6)RNA提取 7)miRNA阵列 B.基于siRNA的基因沉默： 1)设计和合成对TNPRC社区有用的siRNA的子集 2)个体siRNA检测方法的优化和标准化 在2010年，该中心为不同的研究人员进行了大约119次微阵列杂交。其中包括恒河猴微阵列(68)、小鼠微阵列(12)、哺乳动物miRNA微阵列(19)和结核分枝杆菌微阵列(20)。其中一些数据已经发表(4份出版物)，而更多的数据目前正在分析中。已经购买了一台新的微阵列合成器，并将于明年安装。在接下来的几年里，核心希望扩展到以下研究服务领域：定制微阵列：核心将根据需求生产几个定制微阵列。第一批将由CORE设计、制造和生产的阵列将包括恒河猴组织阵列(肠道、大脑)、泛猕猴淋巴芯片和用于监测“无病原体”猕猴群体的多病原体检测芯片。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The DNA Microarray and Expression Core (Microarray Core) at TNPRC currently provides the following services: A. DNA Microarrays: 1) Microarray experimental design: 2) Spotted or 2-color microarray experiments: 3) Microarray Data Analysis 4) Small Sample Amplification 5) PCR Arrays 6) RNA Isolation 7) miRNA arrays B. siRNA based gene-silencing: 1) Design and synthesis of a subset of siRNA's useful to the TNPRC community 2) Optimization and standardization of individual siRNA assay During the year 2010, the core performed approximately 119 microarray hybridizations for various investigators. These include rhesus macaque microarrays (68), mouse microarrays (12), mammalian miRNA microarrays (19) and M. tuberculosis arrays (20). Some of this data has already been published (4 publications), while much more is currently being analyzed. A new microarray synthesizer had been purchased and will be set up in the next year. In the coming years, the core would like to expand into the following research service areas: Custom microarrays: The core will produce several custom microarrays based on demand. The first few arrays that will be designed, fabricated and produced by the core will include rhesus macaque tissue arrays (Gut, brain), a pan-macaque lympho-chip and a multi-pathogen detection chip for surveillance of the "pathogen-free" macaque colony.