DNA MICROARRAY AND EXPRESSION CORE

该子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心赠款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 TNPRC的DNA微阵列和表达核心（微阵列核心）目前提供以下服务： A. DNA微阵列： 第一章 微阵列实验设计： （二） 斑点或双色微阵列实验： 第三章 微阵列数据分析 四、 小样本扩增 第五章） PCR阵列 六、 RNA分离 第七章） miRNA阵列 B。 基于siRNA的基因沉默： 第一章 对TNPRC群体有用的siRNA子集的设计和合成 （二） 单个siRNA测定的优化和标准化 在2010年期间，核心为各种研究者进行了大约119次微阵列杂交。这些包括恒河猴微阵列（68），小鼠微阵列（12），哺乳动物miRNA微阵列（19）和M。结核阵列（20）。其中一些数据已经发表（4篇出版物），而更多的数据目前正在分析中。一个新的微阵列合成器已经购买，并将在明年成立。在未来几年，核心希望扩大到以下研究服务领域：定制微阵列：核心将根据需求生产几个定制微阵列。该核心将设计、制造和生产的前几个阵列将包括恒河猴组织阵列（肠道、大脑）、泛猕猴淋巴芯片和用于监测“无病原体”猕猴群体的多病原体检测芯片。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The DNA Microarray and Expression Core (Microarray Core) at TNPRC currently provides the following services: A. DNA Microarrays: 1) Microarray experimental design: 2) Spotted or 2-color microarray experiments: 3) Microarray Data Analysis 4) Small Sample Amplification 5) PCR Arrays 6) RNA Isolation 7) miRNA arrays B. siRNA based gene-silencing: 1) Design and synthesis of a subset of siRNA's useful to the TNPRC community 2) Optimization and standardization of individual siRNA assay During the year 2010, the core performed approximately 119 microarray hybridizations for various investigators. These include rhesus macaque microarrays (68), mouse microarrays (12), mammalian miRNA microarrays (19) and M. tuberculosis arrays (20). Some of this data has already been published (4 publications), while much more is currently being analyzed. A new microarray synthesizer had been purchased and will be set up in the next year. In the coming years, the core would like to expand into the following research service areas: Custom microarrays: The core will produce several custom microarrays based on demand. The first few arrays that will be designed, fabricated and produced by the core will include rhesus macaque tissue arrays (Gut, brain), a pan-macaque lympho-chip and a multi-pathogen detection chip for surveillance of the "pathogen-free" macaque colony.