Regeneration of Oral Mucosa by Tissue Engineered Oral Mucosa Grafts.

通过组织工程口腔粘膜移植物实现口腔粘膜再生。

• 批准号: 16390581
• 负责人: YOSHIZAWA Michiko
• 金额: $ 3.78万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390581/
• 关键词: regenerative medicine; oral mucosa; oral keratinocyte; 上皮細胞; 組織工学

[Introduction] We have developed an ex vivo produced oral mucosa equivalent (EVPOME) in a serum-free culture system without a feeder layer. EVPOME is composed of a stratified layer of oral keratinocytes seeded onto a human Cadaveric dermis, AlloDerm^<【○!R】>. We had started clinical application of EVPOME since 2000. The healing period and the degree of scar formation seemed to be different depend on the sites of grafting. It is necessary to investigate the mechanism of regeneration of oral mucosa by EVPOME in order to obtain a good healing of oral mucosa. The objective of the present study was to develop the animal model of grafted EVPOME on the dorsum skin and in oral cavity and to evaluate the course after EVPOME grafting histologically. [Materials and Methods] The full thickness defects on the dorsum skin or 5mm^2 defects on the buccal mucosa were created in 4-5 week-old nude mice (BALB/c) and EVPOMEs were grafted on the defect. Grafted EVPOMEs were harvested at 5, 7, 14, 21, and 28 days postgrafting for histological examination. As a control, AlloDerm^<【○!R】> was grafted in those defects. [Results] The epithelial coverage was not seen at day 7 postoperatively in both EVPOME and AlloDerm^<【○!R】> grafting on the dorsum skin in the mice. In intraoral grafting group, epithelium was seen on AlloDerm^<【○!R】> as same as EVPOME cultured in vitro at day 5 postoperatively. A discontinuous basal and suprabasal epithelial layer survived, while the superficial keratinized epithelial layer tended to be peeled off AlloDerm^<【○!R】> at day7 postoperatively. Epithelium was more stratified similar to the adjacent mucosa after dayl4 postoperatively. [Discussion and Conclusion] These results suggested that both of the epithelium and basement membrane in EVPOME survived after intraoral grafting and contributed to regeneration of oral mucosa in nude mice, whereas epithelium of EVPOME was not survived after grafting on the dorsum skin.

[引言]我们在无饲养层的无血清培养系统中开发了离体产生的口腔粘膜等同物（EVPOME）。EVPOME由接种在人尸体真皮上的口腔角质形成细胞的分层组成，AlloDerm^[0！R]>.我们从2000年开始临床应用EVPOME。愈合时间和瘢痕形成的程度似乎因移植部位而异。为获得良好的口腔黏膜愈合，有必要探讨EVPOME促进口腔黏膜再生的机制。本研究的目的是建立EVPOME在背部皮肤和口腔内移植的动物模型，并从组织学上评价EVPOME移植后的病程。【材料与方法】4-5周龄BALB/c裸鼠背部皮肤全层缺损或颊黏膜5mm^2缺损，移植EVPOME。在移植后5、7、14、21和28天收获移植的EVPOME用于组织学检查。作为对照，AlloDerm^<[○！R]>被嫁接在这些缺陷中。[结果]术后第7天，EVPOME组和AlloDerm组均未见上皮覆盖;小鼠背部皮肤移植。在口内移植组中，在AlloDerm^<[○！术后第5天与体外培养的EVPOME相同。不连续的基底和基底上上皮层存活，而浅表角化上皮层倾向于从AlloDerm^<[○！术后第7天R>。术后第14天，上皮分层明显，与邻近粘膜相似。[讨论与结论] EVPOME移植于裸鼠口腔内后，上皮和基底膜均存活，并对口腔黏膜的再生有促进作用，而移植于背部皮肤后，上皮则不能存活。