Destruction of the biological clock system by environmental contaminants : Crosstalk between heme, NO, protein synthesis and clock genes

环境污染物对生物钟系统的破坏：血红素、NO、蛋白质合成和生物钟基因之间的串扰

• 批准号: 17101002
• 负责人: SHIMIZU Toru
• 金额: $ 71.14万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17101002/
• 关键词: NO,ヘム; 環境汚染物質; 時計遺伝子; 蛋白合成; NO; ヘム; ヘム鉄; 転写因子; 環境汚染; シグナル伝達; 水銀; ヘム制御酵素・蛋白質; ガスセンサー; 蛋白質合成; 体内時計; DNA結合; 水銀イオン; NOガス; PASドメイン; 赤血球; プロテインマイクロアレイ

[1] Molecular mechanisms of NO- and heme-sensing of heme-regulated kinase (HRI) and heme-regulated transcriptional factors were elucidated and mercury was found to break the heme-protein interactions.; [2] Thiolate and α-lipoic acid remedied depressive behavior of mice induced by methyl-mercury.; [3] Gas-recognition sites and molecular mechanism of gas-induced catalytic enhancement of the heme-regulated gas-sensor enzymes were elucidated.

血红素调节激酶（HRI）和血红素调节转录因子的NO-和血红素感应的分子机制被阐明，并且发现汞可以破坏血红素-蛋白相互作用。硫代酸和α-硫辛酸对甲基汞致小鼠抑郁行为的修复作用阐明了血红素调节的气体传感酶的气体识别位点和气体诱导催化增强的分子机制。

项目成果

Role of Phe113 at the distal side of the heme domain of an oxygen-sensor(Ec DOS)in the characterization of the heme environment

氧传感器 (Ec DOS) 血红素域远端 Phe113 在血红素环境表征中的作用

• 发表时间: 2009
• 期刊: Journal of Inorganic Biochemistry 103
• 作者: Ito;Shinya;Araki;Yasuyuki;Tanaka;Atsunari;Igarashi;Jotaro;Wada;Takehiko;Shimizu;Toru
• 通讯作者: Toru

Site-directed mutagenesis study to identify the heme binding site…

定点诱变研究以确定血红素结合位点......

• 发表时间: 2007
• 作者: 村瀬元彦;他
• 通讯作者: 他

Characterization of Cry, an FAD-binding protein associated with…

Cry 的表征，一种与……相关的 FAD 结合蛋白

• 发表时间: 2007
• 作者: 石川静;他
• 通讯作者: 他

Critical role of the heme axial ligand, Met95, in locking catalysis of the phosphodiesterase from Escherichia coli (Ec DOS) toward cyclic diGMP

• DOI: 10.1074/jbc.m701920200
• 发表时间: 2007-07-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Tanaka, Atsunari;Takahashi, Hiroto;Shimizu, Toru
• 通讯作者: Shimizu, Toru

Spectroscopic characterization of the isolated heme-bound PAS-B domain of neuronal PAS domain protein 2 associated with circadian rhythms

• DOI: 10.1111/j.1742-4658.2005.04828.x
• 发表时间: 2005-08-01
• 期刊: FEBS JOURNAL
• 影响因子: 5.4
• 作者: Koudo, R;Kurokawa, H;Shimizu, T
• 通讯作者: Shimizu, T