Development of artificial arrayed nose system with drug metabolizing enzymes
药物代谢酶人工阵列鼻系统的开发
基本信息
- 批准号:17300058
- 负责人:
- 金额:$ 10.42万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A novel artificial arrayed nose system with drug metabolizing system was investigated with following steps,(a) optical fluorescent fiber with alcohol dehydrogenase for ethanol vapor,(b) biochemical sniffer with flavin containing monooxygenase as one of the drug metabolizing enzyme,(c) bio-sniffer chip with screen printed electrodes. The detailed results are described as follows.(a) The fiber optic type biochemical gas-sensor(bio-sniffer) was constructed by using NAD+ dependent dehydrogenase enzyme and exciting light source of UV-LED and portable spectrometer: In this sensor system, the concentration of ethanol vapor could be successfully measured by detecting the fluorescent change in NADH as ADH (alcohol dehydrogenase) enzymatic product by the portable fiber optic fluorometric system. The fluorescent sniffer system could be applied to other sniffers for other smell chemicals (acetaldehyde, formaldehyde, lactate, pyruvate, succinate, etc) using their dehydrogenase enzymes. The thinner … More fiber would be suitable for constructing the arrayed nose system for multi analyte in the smell.(b) Triethylamine (TEA) is a colorless volatile organic compounds with a strong fishy malodor, like ammonia, in the gas phase. A bioelectronic sniffer for triethylamine (TEA) was developed with a flavin-containing monooxygenase type 3 (FMO-3). The TEA biosniffer consisted of a Clarktype dissolved-oxygen electrode and an FMO-3 immobilized membrane. In order to amplify the biosensor output was applied. The FMO-3 biosniffer was used to measure TEA vapor.(c) acetaldehyde is one of the specific malodor substances, and its maximum permitted concentration is defined as 25 ppm by ACGIH. The bio-sniffer device was constructed by coating a carbon and Ag/AgCl electrodes on a filter paper, and immobilizing enzyme (ALDH) to the carbon electrode. The ALDH sniffer was used in the gas phase to measure acetaldehyde vapor with the calibration range from 0.2 to 4.0 ppm with good gas selectivity which was attributed to the substrate specificity of ALDH. Less
研究了一种新型的具有药物代谢系统的人工阵列鼻系统,包括以下步骤:(A)乙醇蒸气的乙醇脱氢酶荧光光纤;(B)以黄素单加氧酶为药物代谢酶之一的生化嗅探器;(C)丝网印刷电极的生物嗅探芯片。具体研究结果如下:(1)利用依赖NAD+的脱氢酶,以UV-LED和便携式光谱仪为激发光源,构建了光纤型生化气体传感器(生物嗅探器):在该传感器系统中,通过便携式光纤荧光系统检测乙醇蒸气中NADH作为乙醇脱氢酶(ADH)酶解产物的荧光变化,成功地测量了乙醇蒸气的浓度。该荧光嗅探系统可应用于其他气味化学物质(乙醛、甲醛、乳酸、丙酮酸、丁二酸等)的脱氢酶嗅探。更薄的…(B)三乙胺(TEA)是一种无色挥发性有机化合物,与气相中的氨一样,具有强烈的鱼腥味。利用含有黄素的单加氧酶3(FMO-3)研制了三乙胺(TEA)生物电子嗅探仪。茶叶生物滤器由Clark型溶氧电极和FMO-3固定化膜组成。为了放大生物传感器,采用了输出信号。(C)乙醛是一种特殊的恶臭物质,其最大允许浓度被ACGIH定义为25ppm。将碳电极和银/氯化银电极涂覆在滤纸上,将酶(ALDH)固定在碳电极上,构建了生物嗅探器。利用ALDH的底物专属性,在气相中使用ALDH嗅探器测定乙醛蒸气,校正范围为0.2~4.0ppm,具有良好的气体选择性。较少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
BREATH ANALYSIS WITH BIO-SNIFFERS(BIOCHEMICAL GAS SENSORS) FOR ETHANOL AND ACETALDEHYDE AFTER DRINKING
使用生物嗅探器(生化气体传感器)对酒后乙醇和乙醛进行呼吸分析
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Hiroyuki Matsunagaa;Hirokazu Saito b;Hiroyuki Kudo b and Kohji Mitsubayashi
- 通讯作者:Hiroyuki Kudo b and Kohji Mitsubayashi
BIOELECTRONIC SNIFFERS FOR FORMALDEHYDE IN THE GAS PHASE
气相甲醛生物电子嗅探器
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Kohji MITSUBAYASHI;et. al.
- 通讯作者:et. al.
An NADH fluorometric biosensor for ethanol measurement
用于乙醇测量的 NADH 荧光生物传感器
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Kohji MITSUBAYASHI;et. al.
- 通讯作者:et. al.
A bio-sniffer stick with FALDH (formaldehyde dehydrogenase) for convenient analysis of gaseous formaldehyde
- DOI:10.1016/j.snb.2007.07.086
- 发表时间:2008-03-14
- 期刊:
- 影响因子:8.4
- 作者:Mitsubayashi, Kohji;Nishio, Genki;Marty, Jean-Louis
- 通讯作者:Marty, Jean-Louis
Biochemical sniffer for odourless H2O2 vapour
无味 H2O2 蒸气生化嗅探器
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:KIMIO OTSUKA;TERUYOSHI GOTO;HIROKAZU AMAGAI;NAOKO ISHII;HIDEAKI ENDO and KOHJI MITSUBAYASHI
- 通讯作者:HIDEAKI ENDO and KOHJI MITSUBAYASHI
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MITSUBAYASHI Kohji其他文献
Mouthguard type biosensor for wireless measurement of salivary uric acid
用于无线测量唾液尿酸的护齿式生物传感器
- DOI:
10.2978/jsas.34301 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
KAWASE Gentaro;OISHI Takumi;IITANI Kenta;TOMA Koji;ARAKAWA Takahiro;MITSUBAYASHI Kohji - 通讯作者:
MITSUBAYASHI Kohji
ボロブドゥール寺院壁面レリーフの3次元復元のための学習データセット作成支援法
婆罗浮屠寺庙墙壁浮雕3D重建的学习数据集创建支持方法
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
KUBOTERA Tomoya;OISHI Takumi;ARAKAWA Takahiro;TOMA Koji;MITSUBAYASHI Kohji;JI Shenyu,LI Liang,長谷川 恭子,田中 覚 - 通讯作者:
JI Shenyu,LI Liang,長谷川 恭子,田中 覚
Uric acid mouthpiece biosensor integrated with Bluetooth low energy wireless module for salivary uric acid measurement
尿酸咬嘴生物传感器集成蓝牙低功耗无线模块,用于唾液尿酸测量
- DOI:
10.2978/jsas.33301 - 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
KUBOTERA Tomoya;OISHI Takumi;ARAKAWA Takahiro;TOMA Koji;MITSUBAYASHI Kohji - 通讯作者:
MITSUBAYASHI Kohji
Unrestrained and Continuous Biosensing for Medical and Health Sciences
适用于医疗和健康科学的无限制连续生物传感
- DOI:
10.2493/jjspe.87.907 - 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
Sumitani Shinji;Suzuki Reiji;Arita Takaya;Nakadai Kazuhiro;Okuno Hiroshi G.;斎藤環;MITSUBAYASHI Kohji - 通讯作者:
MITSUBAYASHI Kohji
Headset-type Ear Canal Gas Sensor for Monitoring of Volatile Organic Compounds in Human Blood
用于监测人体血液中挥发性有机化合物的耳机式耳道气体传感器
- DOI:
10.1541/ieejjournal.142.564 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
ARAKAWA Takahiro;MITSUBAYASHI Kohji - 通讯作者:
MITSUBAYASHI Kohji
MITSUBAYASHI Kohji的其他文献
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{{ truncateString('MITSUBAYASHI Kohji', 18)}}的其他基金
Odorless chemical code and imaging (Camera for odorless volatiles)
无味化学编码和成像(无味挥发物相机)
- 批准号:
15K12060 - 财政年份:2015
- 资助金额:
$ 10.42万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
3D-imaging system for odor chemicals (volatile compounds)
用于气味化学物质(挥发性化合物)的 3D 成像系统
- 批准号:
24650078 - 财政年份:2012
- 资助金额:
$ 10.42万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
High sensitive fluorometric biosniffer based on UV-LED emission light and metabolizing enzyme
基于 UV-LED 发射光和代谢酶的高灵敏度荧光生物嗅探器
- 批准号:
23300068 - 财政年份:2011
- 资助金额:
$ 10.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Autonomous flowing tube system for chemical solution propelled by its self-energy
自能驱动的化学溶液自主流动管道系统
- 批准号:
21650041 - 财政年份:2009
- 资助金额:
$ 10.42万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Biochemical gas sensors with drug metabolizing system based on body deodorant function
基于人体除臭功能的具有药物代谢系统的生化气体传感器
- 批准号:
20300062 - 财政年份:2008
- 资助金额:
$ 10.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on a smell communication system using integrated bio-devices
利用集成生物器件的气味通讯系统的研究
- 批准号:
12650396 - 财政年份:2000
- 资助金额:
$ 10.42万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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