Toxicity test by use of 4-element hybrid strain derived from a Japanese wild rat NIG-III

远交系大鼠广泛用于新开发药物的安全性测试，然而，远交系啮齿动物的遗传监测和遗传分析现在不是一件容易的事情。因此，作为毒性最好的工具，我们开发了一种来自日本野生大鼠NIG-III的4元素杂交体。NIG-III系是本实验室于20世纪60年代首次饲养的大鼠近交系，本研究成功地在DN、F344、NIG-III和SHR四个近交系中建立了多态性微卫星标记。此外，为了验证四元杂交后代遗传监测和遗传分析的准确性和有效性，我们用DN、F344、NIG-III和SHR四个近交系配制了一组杂交后代，测定了它们的出生体重。

Outbred rats are widely used in safety tests for newly developed drugs, however, genetic monitoring and genetic analysis in outbred rodents is not an easy task now Thus, as a tool for toxicity best, we developed a 4-element hybrid derived from a Japanese wild rat NIG-III. The strain NIG-III was originally raised in our facility in 1960s.Indeed, we successfully developed rat polymorphic microsatellite markers among inbred strains DN, F344, NIG-III and SHR. Furthermore, in order to demonstrate the accuracy and efficacy of genetic monitoring and genetic analysis in a 4-element hybrid strain, we generated a set of hybrid derived from 4 inbred strains DN, F344, NIG-III and SHR and measured their body weights at birth We could easily determined genotypes of the 4-element hybrid individuals, however, QTL analysis on their body weight at birth was unsuccessful.