Toxicity test by use of 4-element hybrid strain derived from a Japanese wild rat NIG-III

点击翻译按钮获取中文摘要

Outbred rats are widely used in safety tests for newly developed drugs, however, genetic monitoring and genetic analysis in outbred rodents is not an easy task now Thus, as a tool for toxicity best, we developed a 4-element hybrid derived from a Japanese wild rat NIG-III. The strain NIG-III was originally raised in our facility in 1960s.Indeed, we successfully developed rat polymorphic microsatellite markers among inbred strains DN, F344, NIG-III and SHR. Furthermore, in order to demonstrate the accuracy and efficacy of genetic monitoring and genetic analysis in a 4-element hybrid strain, we generated a set of hybrid derived from 4 inbred strains DN, F344, NIG-III and SHR and measured their body weights at birth We could easily determined genotypes of the 4-element hybrid individuals, however, QTL analysis on their body weight at birth was unsuccessful.