Effects of oxygen consumption by arteriolar walls on oxygen transport to tissue under physiological condition

生理条件下小动脉壁耗氧量对组织氧运输的影响

• 批准号: 17300143
• 负责人: SHIBATA Masahiro
• 金额: $ 6.85万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300143/
• 关键词: microcirculation; arterioles; oxygen consumption; vasodilation; nitric oxide; skeletal muscle; 血管平滑筋; 血流

To examine the formation of pO_2 gradients in arterioles, the oxygen consumption rates of arteriolar walls were determined under physiological conditions. A phosphorescence quenching technique was used to quantify the intra- and perivascular pO_2 values in rat cremaster arterioles. Using the measured pO_2 values, and a theoretical model, the O_2 consumption rates of the arteriolar walls were then estimated. We found that the O_2 consumption rate of functional arterioles was 100 times greater than that seen in in vitro experiments, and the O_2 consumption rate of the arteriolar walls in resting skeletal muscle was significantly higher than that during vasodilation. Furthermore, the O_2 consumption rate was the highest in the 1A arterioles, which are located upstream where the blood pressure is highest. These findings suggest that the high O_2 consumption rates of arteriolar walls depend on the workload of the smooth muscle, rather than the endothelium. These results confirm the strong involvement of O_2 consumption by the arteriolar wall itself in the formation of pO_2 gradients in arterioles. Furthermore, an important physiological function of arterioles was demonstrated: large amounts of O_2 are consumed by the vessel walls in resting skeletal muscle arterioles, enabling the arterioles to restrict blood perfusion into skeletal muscle. Consequently, an adequate blood supply to the other organs is ensured, even with a limited systemic blood flow. During exercise, on the other hand the arteriolar O_2 consumption decreases as a result of the relaxation of the vascular smooth muscle, thereby efficiently supplying O_2 to the skeletal muscle.

在生理条件下测定微动脉壁的耗氧率，以观察微动脉内pO_2梯度的形成。用磷光猝灭法测定大鼠提睾肌小动脉血管内和血管周围的pO_2值。根据测得的pO_2值和理论模型，估算了动脉壁的耗氧率。我们发现功能性小动脉的耗氧率比离体实验中所见的高100倍，静息状态下骨骼肌小动脉壁的耗氧率明显高于血管舒张时。1A小动脉的耗氧率最高，位于血压最高的上游。提示小动脉壁的高耗氧率取决于平滑肌的工作负荷，而不是内皮。这些结果证实了动脉壁本身的O_2消耗在动脉内pO_2梯度的形成中的强烈参与。此外，还证实了微动脉的一个重要生理功能：静息状态下骨骼肌微动脉的血管壁消耗大量的O_2，使微动脉限制骨骼肌的血液灌注。因此，即使在有限的全身血流的情况下，也确保了对其他器官的充足的血液供应。运动时，由于血管平滑肌松弛，小动脉耗氧量减少，从而有效地向骨骼肌供氧。

项目成果

Estimating oxygen consumption rates of arteriolar walls under physiological conditions in rat skeletal muscle

• DOI: 10.1152/ajpheart.00830.2004
• 发表时间: 2005-07-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
• 影响因子: 4.8
• 作者: Shibata, M;Ichioka, S;Kamiya, A
• 通讯作者: Kamiya, A

Nitric oxide modulates oxygen consumption by arteriolar walls in rat skeletal muscle

一氧化氮调节大鼠骨骼肌小动脉壁的耗氧量

• 发表时间: 2005
• 期刊: Am J. Physiol Heart Circ Physiol 289
• 作者: M.Shibata;et al.
• 通讯作者: et al.

Vascular wall energetics in arterioles during nitric oxide-dependent and -independent vasodilation

• DOI: 10.1152/japplphysiol.01632.2005
• 发表时间: 2006-06-01
• 期刊: JOURNAL OF APPLIED PHYSIOLOGY
• 影响因子: 3.3
• 作者: Shibata, Masahiro;Qin, Kairong;Kamiya, Akira
• 通讯作者: Kamiya, Akira

Compact capillaroscopy for human skin using CCD video-probe.

使用 CCD 视频探头对人体皮肤进行紧凑型毛细管镜检查。

• 发表时间: 2007
• 期刊: Microvascular Reviews and Communications 1
• 作者: M.Shibata;N.Ohura;N.Sekiya;S.Ichioka;T.Yamakoshi;K.Yamakoshi;A.Kamiya
• 通讯作者: A.Kamiya

Efficacy of a skin-protection power for use as a dressing for intractable ulcers

用作顽固性溃疡敷料的皮肤保护剂的功效

• 发表时间: 2006
• 期刊: J Wound Care 15
• 作者: N.Ohura;M.Shibata;et al.
• 通讯作者: et al.