In vivo study for non-linear relationship between capillary blood flow and tissue pO_2 in skeletal muscle

骨骼肌毛细血管血流量与组织pO_2非线性关系的体内研究

• 批准号: 15300156
• 负责人: SHIBATA Masahiro
• 金额: $ 6.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15300156/
• 关键词: oxygen; arterioles; capillaries; microcirculation; skeletal muscle; 骨格筋; 酸素輸送; 血流調節; 酸素感受性色素

We tested the hypothesis that skeletal muscle may have a simple, intrinsic O_2-sensing mechanism for regulating capillary blood flow in which capillary flow is started and stopped in response to a threshold pO_2 value. This hypothesis was evaluated by measuring the capillary red blood cell (RBC) velocity and perfused capillary density in response to changes in the pO_2 level of suffusates in rabbit tenuissimus muscle using an intravital microscope. Both the RBC velocity and the capillary density significantly decreased as the pO_2 level of the suffusates was elevated, but the relationship between the capillary blood flow rate, calculated by the velocity and density data, and the pO_2 level of the suffusates displayed a non-linear correlation. This data suggests that a critical pO_2 value affecting microvascular blood flow regulation might exist for suffusates with a pO_2 of between 35 and 45 Torr. Above or below this critical threshold pO_2 level, capillary blood flow drastically decreases or increases. These findings support the possibility that the arterioles play an important role in detecting local metabolic demand and directly regulate capillary blood flow by influencing the tonus of their smooth muscle, without requiring signal transmission from a distal site.

我们验证了骨骼肌可能具有一种简单的、内在的氧敏感机制来调节毛细血管血流的假设，即毛细血管血流的启动和停止响应于阈值pO_2值。用活体显微镜测定了家兔细薄肌毛细血管红细胞（RBC）流速和灌注毛细血管密度随pO_2水平变化的变化。红细胞流速和毛细血管密度均随pO_2的升高而显著降低，但毛细血管流速与pO_2的关系呈非线性相关。这些数据表明，在pO_2为35 ~ 45 Torr的充氧液中，可能存在一个影响微血管血流调节的临界pO_2值。高于或低于这个临界阈值pO_2水平，毛细血管血流量急剧减少或增加。这些发现支持了这样一种可能性，即小动脉在检测局部代谢需求方面发挥重要作用，并通过影响其平滑肌的张力直接调节毛细血管血流，而不需要来自远端部位的信号传输。

项目成果

A symmetrical dorsal island double flap in the mouse.

小鼠的对称背岛双瓣。

• 发表时间: 2004
• 期刊: Eur J Plast Surg 27
• 作者: S.Ichioka;S.Kudo;K.Tokioka;M.Shibata;et al.
• 通讯作者: et al.

Nonlinear regulation of capillary perfusion in relation to ambient pO2 changes in skeletal muscle

• DOI: 10.1007/s00421-005-1315-6
• 发表时间: 2005-06-01
• 期刊: EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
• 影响因子: 3
• 作者: Shibata, M;Ichioka, S;Kamiya, A
• 通讯作者: Kamiya, A

Ohura, Shibata, et al.: "Global analysis of shear stress-responsive genes in vascular endothelial cells"Journal of Atherosclerosis & Thrombosis. 10(5). 304-313 (2003)

Ohura、Shibata 等人：“血管内皮细胞中剪切应力反应基因的全局分析”动脉粥样硬化杂志

柴田, 市岡, 安藤, 神谷, 戸川: "骨格筋微小循環における酸素感知と血流制御"生体医工学. 41(特別号). 288 (2003)

Shibata、Ichioka、Ando、Kamiya、Tokawa：“骨骼肌微循环中的氧传感和血流控制”生物医学工程 41（特刊）288（2003）。

Sprouting from arteriovenous shunt vessels with increased blood flow

• DOI: 10.1007/bf02345133
• 发表时间: 2005-01-01
• 期刊: MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
• 影响因子: 3.2
• 作者: Asano, Y;Ichioka, S;Nakatsuka, T
• 通讯作者: Nakatsuka, T