Energetics in arterioles during nitric oxide dependent and independent vasodilation

一氧化氮依赖性和独立血管舒张期间小动脉的能量

• 批准号: 19300154
• 负责人: SHIBATA Masahiro
• 金额: $ 11.4万
• 依托单位: Shibaura Institute of Technology (2008-2009)The University of Tokyo (2007)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300154/
• 关键词: 生体システム; フィジオーム; 生物・生体工学; 細胞・組織; 循環器・高血圧; 生理学; 微小循環; 酸素輸送; 毛細血管; 生体顕微鏡

The objective of this study was to evaluate whether the nitric oxide (NO) would decrease vessel wall oxygen consumption by decreasing the mechanical work of vascular smooth muscle. The oxygen consumption rate (QO_2) of arteriolar walls in rat cremaster muscle was determined in vivo during NO dependent and -independent vasodilation based on the intra- and perivascular oxygen tension (PO_2) measured by phosphorescence quenching technique. NO dependent vasodilation was induced by increased NO production due to increased blood flow, while NO independent vasodilation was induced by topical administration of papaverine. The energy efficiency was evaluated by the variable ratio of wall tension to QO_2 between normal and vasodilated conditions. NO dependent and -independent dilation increased arteriolar diameters by 13% and 17%, respectively. Vascular wall QO_2 decreased significantly during both dilations. There was no significant difference between the energy efficiency during NO dependent and -independent vasodilation, suggesting the decrease in vascular wall QO_2 produced by NO to be related to a decrease in the mechanical work of vascular smooth muscle.

本研究的目的是探讨一氧化氮(NO)是否通过降低血管平滑肌的机械功来降低血管壁耗氧量。用磷光猝灭法测定血管内和血管周围氧分压(PO_2)，测定NO依赖性和非依赖性血管扩张过程中大鼠提睾肌小动脉壁耗氧速率(QO_2)。局部应用罂粟碱不能引起非依赖性血管扩张，但可通过增加血流量而增加NO的产生来诱导非依赖性血管扩张。能量效率通过正常和血管扩张状态下的室壁张力与QO2的可变比率来评价。NO依赖扩张和非依赖扩张分别使小动脉直径增加13%和17%。血管壁QO2在两次扩张过程中均显著降低。NO依赖性和非依赖性血管扩张时的能量效率无显著差异，提示NO引起的血管壁QO_2降低可能与血管平滑肌的机械功减少有关。

项目成果

A technique to visualize wound bed microcirculation and the acute effect of negative pressure

• DOI: 10.1111/j.1524-475x.2008.00390.x
• 发表时间: 2008-05-01
• 期刊: WOUND REPAIR AND REGENERATION
• 影响因子: 2.9
• 作者: Ichioka, Shigeru;Watanabe, Hiromi;Nakatsuka, Takashi
• 通讯作者: Nakatsuka, Takashi

Effect of vibration on skin blood flow in an in vivo microcirculatory model.

• 发表时间: 2007-12
• 期刊: Bioscience trends
• 影响因子: 5.5
• 作者: G. Nakagami;H. Sanada;N. Matsui;A. Kitagawa;Hideki Yokogawa;Naomi Sekiya;S. Ichioka;J. Sugama;M. Shibata

Physiological role of nitric oxide in oxygen consumption by arteriolar wall.

一氧化氮在小动脉壁耗氧量中的生理作用。

• 发表时间: 2008
• 期刊: Conf Proc IEEE Eng Med Biol Soc
• 作者: Shibata M;Yamakoshi T;Yamakoshi K
• 通讯作者: Yamakoshi K

Energetics in arterioles during nitric oxide dependent and independent vasodilation.

一氧化氮依赖性和独立血管舒张期间小动脉的能量。

• 发表时间: 2008
• 作者: Shibata M;Yamakoshi T;Yamakoshi K
• 通讯作者: Yamakoshi K

Nitric oxide contribution to vascular wall oxygen consumption in arterioles.

一氧化氮对小动脉血管壁耗氧量的贡献。

• 发表时间: 2007
• 作者: Shibata M;Ohura N;Sekiya N;Ichioka S;Kamiya A
• 通讯作者: Kamiya A