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Construction of artificial symbiotic community using Chlorella-symbiont association and it's application

小球藻-共生体人工共生群落的构建及其应用

基本信息

批准号：
17310042
负责人：
TANAKA Hideo
金额：
$ 10.02万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Chlorella sorokiniana IAM C-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. The consortium was separated and four nonalgal constituents, a fungal strain (Acremonium sp.), and three bacterial strains (Ralstonia pickettii, Sphingomonas sp. DD38, Microbacterium trichotecenolyticum) were isolated and identified. The interaction between Chlorella and its symbionts under photoautotrophic conditions involved both mutualism and commensalisms. Sphingomonas sp. And M. trichotecenolyticum were harbored on the sheath produced by Chlorella, while Acremonium sp. And R. pickettii directly adhered to the Chlorella cell surface by using lectin-like substances. The sheath is composed of sugars, amino acids, vitamins and metals. A novel artificial medium that imitates photoautotroph-heterotroph interaction was developed. With the artificial medium, it was possible to selectively isolate some novel bacterial strains. The sheath is a suitable habitat for several symbiotic microorganisms because it ensures close proximity between the C. sorokiniana and symbionts. The sheath was increased by addition of calcium chloride. The sheath resulted in coflocculation of C. sorokiniana and the associated symbiotic bacteria, thus strengthening the bacterial-Chlorella symbiotic association. An application of this technique was demonstrated by constructing a complex of C. sorokiniana and a propionate-degrading bacterium (PDS1). Although propionate inhibited the growth of axenic C. sorokiniana, the C. sorokiniana-PDS1 complex (CPC) showed good growth in a medium containing a high concentration of propionate. The CPC was also used for simultaneous removal of organic acids, nitrate, ammonia and phosphate.

小球藻（Chlorella sorokiniana）IAM C-212作为一种混合菌株长期以来一直保持在斜面培养物中，代表了一种相关的天然微生物聚生体。分离了该聚生体，并分离出四种非藻类成分，一种真菌菌株（枝顶孢属），分离鉴定出3株细菌（皮氏罗尔斯通氏菌、鞘氨醇单胞菌DD 38、溶戊烯微杆菌）。在光合自养条件下，小球藻与其共生体之间的相互作用既有互惠关系，也有共生关系。鞘氨醇单胞菌（Sphingomonasp.）解烯枝顶孢霉（Acremonium sp.）和R. pickettii通过使用凝集素样物质直接粘附在小球藻细胞表面。鞘由糖、氨基酸、维生素和金属组成。开发了一种模拟光合自养-异养生物相互作用的新型人工培养基。利用该人工培养基，有可能选择性地分离出一些新的细菌菌株。鞘是几种共生微生物的合适栖息地，因为它确保了C。sorokiniana和共生体。通过添加氯化钙来增加鞘。鞘的存在导致了C. sorokiniana和相关的共生细菌，从而加强细菌-小球藻共生协会。通过构建C. sorokiniana和丙酸盐降解菌（PDS 1）。丙酸盐对无菌C. sorokiniana、C. sorokiniana-PDS 1复合体（CPC）在含有高浓度丙酸盐的培养基中显示良好的生长。CPC还用于同时去除有机酸、硝酸盐、氨和磷酸盐。