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Generation of transplantable hematopoietic stem cells based from human embryonic stem cells

基于人类胚胎干细胞产生可移植造血干细胞

基本信息

批准号：
17390297
负责人：
TSUJI Kohichiro
金额：
$ 10.33万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390297/
关键词：
human embrvonic stem (ES) cells hematopoietic stem cells hematopoietic stem cell transplantation hematopoietic progenitor cells fetal hematoppiesis fatal liver stroma transfusion とトES細胞多能性造血前駆細胞血小板溶解液多能性造血細胞混合コロニー形成細胞肥満細胞

项目摘要

We proposed a novel method for the efficient production of hematopoietic progenitors from human embryonic stem cells (hESC) by co-culturing with stromal cells derived from murine fetal liver, in which embryonic hematopoiesis drastically expands at midgestation. In the co-culture, various hematopoietic progenitors were generated, and this hematopoietic activity was concentrated in cobblestone-like cells derived from differentiated hESC. The cobblestone-like cells mostly expressed CD34 and retained an endothelial potential. They also contained hematopoietic colony-forming cells, especially erythroid and multilineage colony-forming cells at high frequency. The multipotential hematopoietic progenitors abundant among the cobblestone-like cells produced almost all types of mature blood cells, including adult-type b globin-expressing erythrocytes and tryptase/chymase double-positive mast cells. They showed neither immature properties of ESC nor the potential to differentiate into endoderm and ectoderm at a clonal level. The developed co-culture system of hESC can provide a novel source for hematopoietic and blood cells applicable to cellular therapies and drug screenings.

我们提出了一种新的方法，用于从人胚胎干细胞（hESC）的造血祖细胞的有效生产与基质细胞来自小鼠胎肝，其中胚胎造血急剧扩大在妊娠中期共培养。在共培养中，产生了各种造血祖细胞，并且这种造血活性集中在来源于分化的hESC的鹅卵石样细胞中。鹅卵石样细胞主要表达CD 34，并保留内皮细胞的潜力。它们还含有造血集落形成细胞，特别是红系和多系集落形成细胞的高频率。鹅卵石样细胞中丰富的多能造血祖细胞产生几乎所有类型的成熟血细胞，包括成人型B球蛋白表达红细胞和类胰蛋白酶/糜蛋白酶双阳性肥大细胞。它们既没有表现出ESC的不成熟特性，也没有在克隆水平分化成内胚层和外胚层的潜力。人胚胎干细胞共培养体系的建立为造血细胞和血细胞的培养提供了新的来源，可用于细胞治疗和药物筛选。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Direct development of functionally mature tryptase/ chymase double, positive tissue-type mast cells from primate ES cells.

从灵长类 ES 细胞直接发育功能成熟的类胰蛋白酶/糜酶双阳性组织型肥大细胞。

DOI：
发表时间：
2008
期刊：
Stem Cells 26
影响因子：
0
作者：
朴明子;滝智彦;小田慈;八木啓子;小林良二;鈴木信寛;原純一;堀部敬三;林泰秀;Ma F et al.
通讯作者：
Ma F et al.

Generation of functional erythrocytes from human embryonic stem cell-derived definitive hematopoiesis

DOI：
10.1073/pnas.0802220105
发表时间：
2008-09-02
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Ma, Feng;Ebihara, Yasuhiro;Tsuji, Kohichiro
通讯作者：
Tsuji, Kohichiro

Hematopoietic origins of fibroblasts : II, invitro studies of fibrolasts, CFU-f and fibrocytes.

成纤维细胞的造血起源：II，成纤维细胞、CFU-f 和纤维细胞的体外研究。