Induction of differentiation of monkey embryonic stem cells into tissue-specific functional cells

猴胚胎干细胞诱导分化为组织特异性功能细胞

• 批准号: 12557067
• 负责人: TSUJI Kohichiro
• 金额: $ 8.13万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557067/
• 关键词: monkey embryonic stem cells; AGM region; stromal cells; NOD; SCID mice; 造血幹細胞; ストローマ

1. Characterization of rhesus embryonic stem (rES) cellsrES cells were maintained by the coculture with mouse embryonic fibroblasts. The maintained rES cells expressed alkaliphaphatase, SSEA1 and SSEA4. When the rES cells were transplanted into NOD/SCID mice through the tail vain, they produced teratoma in the recipient's lung. These results indicate the immature nature of rES cells.2. Differentiation of rES cellsWhen the rES cells were cocultured with stromal cells derived from aorta-gonad-mesonephros (AGM) region in 10.5 days postcoitum (dpc) mouse embryo, they formed cobble stone areas, which generated small round cells after 2 weeks of coculture. Flowcytometric analysis showed that a part of the generated cells expressed human CD34. We are further characterizing the cells expressing human CD34.

1.恒河猴胚胎干细胞（rES细胞）的特性通过与小鼠胚胎成纤维细胞共培养来维持rES细胞。维持的rES细胞表达碱性磷酸酶、SSEA 1和SSEA 4。当rES细胞经尾静脉移植到NOD/SCID小鼠体内时，它们在受体的肺中产生了畸胎瘤。这些结果表明rES细胞的不成熟性. rES细胞的分化将rES细胞与胚胎发育后10.5天（dpc）的AGM区基质细胞共培养，形成“鹅卵石区”，共培养2周后，形成小的圆形细胞。流式细胞仪分析显示部分细胞表达人CD 34。我们正在进一步表征表达人CD 34的细胞。

项目成果

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