Induction of differentiation of monkey embryonic stem cells into tissue-specific functional cells

1.用小鼠胚胎成纤维细胞与恒河猴胚胎干细胞共培养，维持恒河猴胚胎干细胞的特性。维持的RES细胞表达碱性磷酸酶、SSEA1和SSEA4。当RES细胞通过尾部无效移植到NOD/SCID小鼠体内时，它们在受者的肺部产生了畸胎瘤。这些结果表明Res细胞的不成熟性质。Res细胞的分化当Res细胞与来自腹主动脉-性腺-中肾(AGM)区的基质细胞在性交后10.5d胚胎共培养时，它们形成鹅卵石区域，2周后形成小圆形细胞。流式细胞仪分析显示，部分生成的细胞表达人CD34。我们正在进一步鉴定表达人CD34的细胞。

1. Characterization of rhesus embryonic stem (rES) cellsrES cells were maintained by the coculture with mouse embryonic fibroblasts. The maintained rES cells expressed alkaliphaphatase, SSEA1 and SSEA4. When the rES cells were transplanted into NOD/SCID mice through the tail vain, they produced teratoma in the recipient's lung. These results indicate the immature nature of rES cells.2. Differentiation of rES cellsWhen the rES cells were cocultured with stromal cells derived from aorta-gonad-mesonephros (AGM) region in 10.5 days postcoitum (dpc) mouse embryo, they formed cobble stone areas, which generated small round cells after 2 weeks of coculture. Flowcytometric analysis showed that a part of the generated cells expressed human CD34. We are further characterizing the cells expressing human CD34.