Establishment of a method inducing embryonic stem cells to differentiate to hematopoietic stem cells (HSC) for therapeutic HSC transplantation

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We challenged to establish a method inducing human embryonic stem (ES) cells to differentiate into hematopoietic stem cells without animal cells and serum for their clinical application. In brief, we first transferred human ES cells onto gelatin-coated culture dishes and cultured with 5% human platelet lysate or serum-containing media to induce their differentiation into stromal cells. Then, human ES cells were cocultured with the estabshished stromal cells under animal serum-free condition for 2 weeks to generate multipotent hematopoietic cells.Thus, we succeeded the establishment of a novel method inducing human ES cells to differentiate into multipotent hematopoietic cells without anima cells and seru,.