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Study of bladder regeneration using acellular matrix graft

脱细胞基质移植膀胱再生的研究

基本信息

批准号：
17390440
负责人：
SHIINA Hiroaki
金额：
$ 6.59万
依托单位：
Shimane University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390440/
关键词：
Bladder regeneration Animal research Acellular matrix graft 大動物

项目摘要

Feasibility of bladder regeneration using bladder acellular matrix graft (BAMG) was investigated. Ina rodent model, acellular matrix as a scaffold was easily applied to induce bladder regeneration even in the diseased host bladder. The process of bladder regeneration within the acellular matrix graft was enhanced after VEGF protein injection into the host bladder, with promising correlation between histological and functional findings. Therefore, these studies demonstrated that normal as well as diseased rat bladder can be successfully augmented with the bladder acellular matrix graft (BAMG). In a porcine model, the bladder regeneration within the BAMG was possible, while the shrinkage of the BAMG appeared to be inevitable. We hypothesized that more chance of providing blood supply to the BAMG could render it possible to accelerate the process involving the bladder regeneration. Next, we investigated whether simultaneous implantation of bilateral ureters onto BAMG can enhance the proce … More ss of bladder regeneration in partial substitution with BAMG. At one week after BAMG implantation, significant inflammatory changes were observed on the host bladder in both groups. No significant endoscopic changes were observed on the luminal surface of BAMG. At two weeks after operation, the inflammatory changes were diminished and epithelization on BMAG was identified, especially close to the host bladder in both groups. Likewise, epithelization on BAMG close to the implanted ureter was observed in ureteral implantation group. At 4 weeks after BAMG implantation, epithelization on BAMG remained in progress in both groups, however; more active and expansive epithelization was found in ureteral implantation group. In a porcine model, endoscopic observation showed that simultaneous implantation of bilateral ureters onto BAMG could enhance the potential of epithelization on the acellular matrix allograft. This new approach using host ureter and bladder as potential source of bladder regeneration can provide the possibility of rapid and complete regeneration of a bladder substitute. Less

探讨膀胱脱细胞基质移植物（BAMG）再生膀胱的可行性。在啮齿动物模型中，脱细胞基质作为支架很容易应用于诱导膀胱再生，即使在患病的宿主膀胱中也是如此。VEGF蛋白注射到宿主膀胱后，脱细胞基质移植物内的膀胱再生过程得到增强，组织学和功能发现之间具有良好的相关性。因此，这些研究表明，正常以及患病大鼠膀胱可以成功地增加与膀胱脱细胞基质移植物（BAMG）。在猪模型中，BAMG内的膀胱再生是可能的，而BAMG的收缩似乎是不可避免的。我们假设，更多的机会提供血液供应的BAMG可以使它有可能加速涉及膀胱再生的过程。接下来，我们研究双侧输尿管同时植入BAMG是否能增强BAMG的治疗效果。 ...更多信息 BAMG部分替代膀胱再生的ss。BAMG植入后1周，两组宿主膀胱均观察到明显的炎症变化。在BAMG的管腔表面上未观察到显著的内窥镜变化。术后2周，两组的炎症改变均减轻，BMAG上均可见上皮形成，尤其是靠近宿主膀胱的部位。输尿管植入组BAMG上靠近植入输尿管处上皮化。BAMG植入后4周，两组BAMG上的上皮化仍在进行中，但输尿管植入组的上皮化更为活跃和扩张。在猪模型中，内窥镜观察显示，双侧输尿管同时植入BAMG可增强脱细胞基质同种异体移植物的上皮化潜力。这种利用宿主输尿管和膀胱作为膀胱再生的潜在来源的新方法可以提供快速和完全再生膀胱替代物的可能性。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Study of bladder regeneration using bladder acellular matrix.

使用膀胱脱细胞基质进行膀胱再生的研究。

DOI：
发表时间：
2006
期刊：
Urology View 4
影响因子：
0
作者：
通讯作者：

Bladder acellular matrixを用いた膀胱再生の検討

使用膀胱脱细胞基质检查膀胱再生

DOI：
发表时间：
2006
期刊：
ウロロジービュー 4巻
影响因子：
0
作者：
Keiko Hama-Tomioka;Hiroyuki Kinoshita;Katsutoshi Nakahata;Ryoko Nakata;Yoshio Hatano;浦上慎司;Shinji Urakami et al.;椎名浩昭
通讯作者：
椎名浩昭

尿路再建における再生医療の現況

再生医学在泌尿道重建中的现状