Mechanism of neo-vascularization and tissue repair for regeneration of head and neck organs

本研究旨在阐明头颈部器官再生过程中新生血管形成和组织修复的机制。培养成纤维细胞、骨髓基质细胞、脂肪组织源性干细胞（ASCs），并用m-YFP标记。将这些细胞与胶原海绵结合，形成生物工程支架。将该支架植入大鼠气管缺损。组织学检查显示成纤维细胞加速了气管缺损的组织修复，而ASCs则诱导了气管缺损的新生血管形成。MSCs与β-TCP结合可获得硬腭组织再生。目前的研究表明，新生血管和组织修复在头颈部器官再生中的重要作用。

The purpose of the present study is to clarify the mechanism of neo-vascularization and tissue repair in the regenerative process of the head and neck organs. Fibroblasts, bone marrow stromal cells, adipose tissue derived stem cells (ASCs) were cultured and labeled by m-YFP. These cells were combined with collagen sponge for developing the bio-engineered scaffold. This scaffold was implanted into the tracheal defect of rats. Histological examination demonstrated that tissue repair of the tracheal defect was accelerated by fibroblasts, while neo-vascularization was induced by ASCs. Tissue regeneration of the hard palate was obtained by combination of MSCs and β-TCP. The current study presented the essential role of neo-vascularization and tissue repair for regeneration of head and neck organs.