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Synaptic plasticity and memory storage in the hippocampal formation of monkeys

猴子海马结构中的突触可塑性和记忆存储

基本信息

批准号：
17500273
负责人：
TAMURA Ryoi
金额：
$ 2.37万
依托单位：
University of Toyama
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17500273/
关键词：
monkeys primates hippocampal formation synaptic plasticity evoked potentials memory lone-term potentiation long-term depression

项目摘要

Long-term potentiation (LTP) and long-term depression (LTD) are both forms of synaptic plasticity which are recognized as a neural substrate for memory. LTP and LTD have been studied extensively in the hippocampus using rodent models. However, there has been no information about synaptic plasticity in the primate hippocampus. Therefore, we addressed this issue in the present study. In the first fiscal year, we exploited a model for recording evoked potentials in the hippocampus of monkeys, precisely implanting stimulation and recording electrodes in the perforant pathway and dentate gyrus, respectively, based on the coordinates determined by MRI images. In the second fiscal year, using this animal model, we tested the effects of high-frequency or low-frequency repetitive stimulation (HFS or LFS) on evoked potentials, and found that only the HFS with the highest frequency (400 Hz) induced LTP, while all LFS conditions failed to induce LTD. In the third fiscal year, we compared the induction and maintenance of LTP between monkeys and rats, and found that LTP was stably maintained more than 1 month without decay in monkeys, while it decayed rapidly and returned to the baseline level within a week in rats. Furthermore, we induced LTP in senile and young monkeys, and tested the relations between LTP induction or maintenance and memory task performance. The senile monkey tended to show worse task performance than the younger monkey. However, no clear correlations between LTP induction or maintenance and task performance were observed. These data indicate that 1) LTP can be a ubiquitous model for long-term synaptic plasticity, and 2) the difference of LTP maintenance between the 2 species is involved in the difference of hippocampal-dependent duration necessary for memory consolidation between the species (i.e., longer in primates and shorter in rodents).

长时程增强(LTP)和长时程增强(LTD)都是突触可塑性的形式，被认为是记忆的神经底物。LTP和LTD已经在海马区使用啮齿动物模型进行了广泛的研究。然而，目前还没有关于灵长类海马区突触可塑性的信息。因此，我们在本研究中解决了这一问题。在第一个财政年度，我们开发了一种记录猴子海马区诱发电位的模型，根据MRI图像确定的坐标，分别在穿孔通路和齿状回分别精确植入刺激和记录电极。在第二个财政年度，我们利用这个动物模型，测试了高频或低频重复刺激(HFS或LFS)对诱发电位的影响，发现只有频率最高的HFS(400 Hz)才能诱导LTP，而LFS条件都不能诱导LTP。在第三财年，我们比较了猴子和大鼠LTP的诱导和维持情况，发现猴子LTP稳定维持1个月以上没有腐烂，而大鼠LTP迅速腐烂并在一周内恢复到基线水平。此外，我们在老年猴和幼猴中诱导了长时程增强，并检验了长时程增强诱导或维持与记忆任务成绩的关系。年迈的猴子往往比年轻的猴子表现出更差的任务表现。然而，LTP的诱导或维持与任务绩效之间没有明显的相关性。这些数据表明：1)LTP可作为长期突触可塑性的普遍模型，2)LTP维持的差异与两种动物记忆巩固所需的海马依赖持续时间的差异有关(即灵长类动物较长，啮齿类动物较短)。