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The development of technique of decrease in nitrate accumulation in vegetables by controlling environmental conditions and nitrate reduction in leaf

通过控制环境条件和叶内硝酸盐还原来减少蔬菜中硝酸盐积累的技术的开发

基本信息

批准号：
17580026
负责人：
NADA Kazuyoshi
金额：
$ 2.46万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The purpose of this study is to establish a technique that would decrease the nitrate concentration in vegetables, especially spinach by using continuous lighting method treated just before harvest (CLM). CLM for 24 hr simultaneously caused a significant decrease in nitrate and increases in ascorbic acid and carotene contents in spinach leaf On the other hands, oxalate which is harmful component content in leaf slightly decreased by CLM Then CLM did not affect an oxalate metabolism in spinach leaf. Therefore, we examined the effects of the combination treatment of CML and carbon dioxide enrichment (CDE) to decrease in nitrate and oxalate simultaneously. Photosynthetic rate of spinach leaf in CLM+CDE condition was 1.6 times higher than in only CLM condition. While, photorespiration rate was almost zero in CLM+CDE condition. This suggests that oxalate metabolism may be seriously inhibited in CLM+CDE condition Nitrate content in spinach leaf decreased by both CLM and CLM+CDE conditions, but the decrease was more pronounced in CLM+CDE than in CLM. CLM treatment did not affect oxalate content in spinach leaf. However, CLM+CDE treatment caused a significant decrease in oxalate content in leaf. The result indicate that combination treatments of continuous lighting and carbon dioxide enrichment treated just before harvest induce simultaneously decrease in harmful components in spinach such as nitrate and oxalate.

本研究的目的是建立一种利用采前连续光照法降低蔬菜，特别是菠菜中硝酸盐浓度的技术。CLM处理24 h后，菠菜叶片中硝酸盐含量显著降低，抗坏血酸和胡萝卜素含量显著升高，叶片中有害成分草酸含量略有下降，不影响叶片中草酸代谢。因此，我们研究了CML和二氧化碳富集（CDE）联合处理同时降低硝酸盐和草酸盐的效果。CLM+CDE处理的菠菜叶片光合速率是单纯CLM处理的1.6倍。而CLM+CDE组光呼吸速率几乎为零。说明CLM+CDE条件下，草酸盐代谢可能受到严重抑制，CLM和CLM+CDE条件下菠菜叶片中硝酸盐含量均下降，但CLM+CDE条件下的下降幅度大于CLM。CLM处理对菠菜叶片中草酸盐含量没有影响。CLM+CDE处理显著降低了叶片草酸盐含量。结果表明，采前连续光照与二氧化碳富集相结合，可使菠菜中硝酸盐和草酸盐等有害成分同时降低。