Relationship between the radio-sensitivity and degree on alteration of nuclear proteins in oral cancer apoptotic cell

口腔癌凋亡细胞放射敏感性与核蛋白改变程度的关系

基本信息

  • 批准号:
    17591971
  • 负责人:
  • 金额:
    $ 2.39万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2005
  • 资助国家:
    日本
  • 起止时间:
    2005 至 2007
  • 项目状态:
    已结题

项目摘要

Background : To investigate the behavior of nuclear proteins in apoptosis induced by protein phosphatase 1 inhibitors, okadaic acid, anti-cancer drugs, and UV radiation in cultured human oral cancer cells.Methods : Dynamic alternations of nucleolin and AgNOR proteins in all inducer-induced apoptosis of cultured human oral cancer cells and in a cell-free apoptotic system were examined using Western blot analysis and immunocytochemical method.Results: The 110-kD_a form of nucleolin and AgNOR protein decreased and the 80-, and 95-kD_a forms appeared during apoptosis in human oral cancer cells and in a cell-free apoptotic system. In addition, the induction of DNA ladder formation coincided with the appearance of alternation of nucleolin and AgNOR proteins in a cell-free apoptosis. Nucleolin diffusely spread out into the nuclear material in the apoptotic body of human oral cancer cells.Conclusions : The present results indicate that alternations of nucleolin and AgNOR proteins are associated with the induction of DNA fragmentation and the final active phase of apoptosis induced by okadaic acid-, anti-cancer drugs, UV radioation in oral cancer cells.
背景资料:为探讨蛋白磷酸酶1抑制剂、冈田酸、抗癌药物和紫外线诱导人口腔癌细胞凋亡过程中核蛋白的行为,采用Western blot和免疫细胞化学方法,检测了不同诱导剂诱导人口腔癌细胞凋亡过程中核仁素和AgNOR蛋白的动态变化。在人口腔癌细胞和无细胞凋亡体系中,110 kD_a型核仁素和AgNOR蛋白减少,80、95 kD_a型核仁素和AgNOR蛋白出现。此外,在无细胞凋亡中,DNA梯状条带的诱导与核仁素和AgNOR蛋白的变化相一致。核仁素扩散到人口腔癌细胞凋亡小体的核物质中。结论:本研究结果表明,核仁素和AgNOR蛋白的变化与诱导口腔癌细胞的DNA断裂和凋亡的最后活动期的诱导冈田酸,抗癌药物,紫外线辐射。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Clinical significance and variation of the advanced calcified stylohyoid complex detected by panoramic radiographs among 80-year-old subjects
80岁老年人全景片检出晚期茎突舌骨复合体钙化的临床意义及变化
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ebisu S;et. al.;Ogawa H et al.;Okabe S et al.
  • 通讯作者:
    Okabe S et al.
Intraoperative surgical clearance conformation of tongue carcinomasusing ultrasonography
舌癌术中超声清除构象
臨床研修歯科医必修マニュアル(竹原直道, 廣藤卓雄 監修)
临床培训牙医必备手册(竹原直道和广人卓夫监督)
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ono K;Inoue H;Masuda;W;Morimoto Y;Tanaka T;Yokota M;Inenaga;K;森本 泰宏
  • 通讯作者:
    森本 泰宏
Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic during growth
下颌骨髁皮质骨形成与生长过程中磁共振信号特征变化的关系
Functional evaluation of parotid glands using dynamic MR sialography with citric acid stimulation.
使用动态 MR 唾液造影和柠檬酸刺激对腮腺进行功能评估。
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Morimoto Y;Okabe S;Yoshioka I;Ono K;Tanaka T;KitoS;Inenaga K
  • 通讯作者:
    Inenaga K
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MORIMOTO Yasuhiro其他文献

MORIMOTO Yasuhiro的其他文献

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{{ truncateString('MORIMOTO Yasuhiro', 18)}}的其他基金

Significance of functional MR images in prognostic evaluation for treatments as treatment of xerostomia
功能性 MR 图像在口干症治疗预后评估中的意义
  • 批准号:
    21592395
  • 财政年份:
    2009
  • 资助金额:
    $ 2.39万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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