Relationship between the radio-sensitivity and degree on alteration of nuclear proteins in oral cancer apoptotic cell
口腔癌凋亡细胞放射敏感性与核蛋白改变程度的关系
基本信息
- 批准号:17591971
- 负责人:
- 金额:$ 2.39万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Background : To investigate the behavior of nuclear proteins in apoptosis induced by protein phosphatase 1 inhibitors, okadaic acid, anti-cancer drugs, and UV radiation in cultured human oral cancer cells.Methods : Dynamic alternations of nucleolin and AgNOR proteins in all inducer-induced apoptosis of cultured human oral cancer cells and in a cell-free apoptotic system were examined using Western blot analysis and immunocytochemical method.Results: The 110-kD_a form of nucleolin and AgNOR protein decreased and the 80-, and 95-kD_a forms appeared during apoptosis in human oral cancer cells and in a cell-free apoptotic system. In addition, the induction of DNA ladder formation coincided with the appearance of alternation of nucleolin and AgNOR proteins in a cell-free apoptosis. Nucleolin diffusely spread out into the nuclear material in the apoptotic body of human oral cancer cells.Conclusions : The present results indicate that alternations of nucleolin and AgNOR proteins are associated with the induction of DNA fragmentation and the final active phase of apoptosis induced by okadaic acid-, anti-cancer drugs, UV radioation in oral cancer cells.
背景资料:为探讨蛋白磷酸酶1抑制剂、冈田酸、抗癌药物和紫外线诱导人口腔癌细胞凋亡过程中核蛋白的行为,采用Western blot和免疫细胞化学方法,检测了不同诱导剂诱导人口腔癌细胞凋亡过程中核仁素和AgNOR蛋白的动态变化。在人口腔癌细胞和无细胞凋亡体系中,110 kD_a型核仁素和AgNOR蛋白减少,80、95 kD_a型核仁素和AgNOR蛋白出现。此外,在无细胞凋亡中,DNA梯状条带的诱导与核仁素和AgNOR蛋白的变化相一致。核仁素扩散到人口腔癌细胞凋亡小体的核物质中。结论:本研究结果表明,核仁素和AgNOR蛋白的变化与诱导口腔癌细胞的DNA断裂和凋亡的最后活动期的诱导冈田酸,抗癌药物,紫外线辐射。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Clinical significance and variation of the advanced calcified stylohyoid complex detected by panoramic radiographs among 80-year-old subjects
80岁老年人全景片检出晚期茎突舌骨复合体钙化的临床意义及变化
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Ebisu S;et. al.;Ogawa H et al.;Okabe S et al.
- 通讯作者:Okabe S et al.
Intraoperative surgical clearance conformation of tongue carcinomasusing ultrasonography
舌癌术中超声清除构象
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Tominaga K;et. al.
- 通讯作者:et. al.
臨床研修歯科医必修マニュアル(竹原直道, 廣藤卓雄 監修)
临床培训牙医必备手册(竹原直道和广人卓夫监督)
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Ono K;Inoue H;Masuda;W;Morimoto Y;Tanaka T;Yokota M;Inenaga;K;森本 泰宏
- 通讯作者:森本 泰宏
Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic during growth
下颌骨髁皮质骨形成与生长过程中磁共振信号特征变化的关系
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Morimoto;Y.
- 通讯作者:Y.
Functional evaluation of parotid glands using dynamic MR sialography with citric acid stimulation.
使用动态 MR 唾液造影和柠檬酸刺激对腮腺进行功能评估。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Morimoto Y;Okabe S;Yoshioka I;Ono K;Tanaka T;KitoS;Inenaga K
- 通讯作者:Inenaga K
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MORIMOTO Yasuhiro其他文献
MORIMOTO Yasuhiro的其他文献
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{{ truncateString('MORIMOTO Yasuhiro', 18)}}的其他基金
Significance of functional MR images in prognostic evaluation for treatments as treatment of xerostomia
功能性 MR 图像在口干症治疗预后评估中的意义
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21592395 - 财政年份:2009
- 资助金额:
$ 2.39万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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