Relationship between the radio-sensitivity and degree on alteration of nuclear proteins in oral cancer apoptotic cell

背景：探讨核蛋白在蛋白磷酸酶1抑制剂、冈田酸、抗癌药物和紫外线诱导人口腔癌细胞凋亡中的作用。方法：采用Western印迹分析和免疫细胞化学方法检测核仁蛋白和AgNOR蛋白在各种诱导剂诱导人口腔癌细胞凋亡和无细胞凋亡系统中的动态变化。结果：口腔癌细胞凋亡过程中核仁蛋白和AgNOR蛋白110-kD_a减少，80-kD_a和95-kD_a形成。此外，DNA梯带的诱导与核仁蛋白和AgNOR蛋白在无细胞凋亡中的交替出现是一致的。结论：核仁蛋白和AgNOR蛋白的改变与冈田酸、抗癌药物、紫外线照射诱导口腔癌细胞DNA断裂和最终的凋亡活跃期有关。

Background : To investigate the behavior of nuclear proteins in apoptosis induced by protein phosphatase 1 inhibitors, okadaic acid, anti-cancer drugs, and UV radiation in cultured human oral cancer cells.Methods : Dynamic alternations of nucleolin and AgNOR proteins in all inducer-induced apoptosis of cultured human oral cancer cells and in a cell-free apoptotic system were examined using Western blot analysis and immunocytochemical method.Results: The 110-kD_a form of nucleolin and AgNOR protein decreased and the 80-, and 95-kD_a forms appeared during apoptosis in human oral cancer cells and in a cell-free apoptotic system. In addition, the induction of DNA ladder formation coincided with the appearance of alternation of nucleolin and AgNOR proteins in a cell-free apoptosis. Nucleolin diffusely spread out into the nuclear material in the apoptotic body of human oral cancer cells.Conclusions : The present results indicate that alternations of nucleolin and AgNOR proteins are associated with the induction of DNA fragmentation and the final active phase of apoptosis induced by okadaic acid-, anti-cancer drugs, UV radioation in oral cancer cells.